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UniProtKB/Swiss-Prot entry Q14914


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PTGR1_HUMAN
Primary accession number Q14914
Secondary accession numbers Q8IYQ0 Q9H1X6
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on September 19, 2002 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 80)
Name and origin of the protein
Protein name Prostaglandin reductase 1
Synonyms PRG-1
EC 1.3.1.-
NADP-dependent leukotriene B4 12-hydroxydehydrogenase
EC 1.3.1.74
15-oxoprostaglandin 13-reductase
EC 1.3.1.48
Gene name
Name: PTGR1
Synonyms: LTB4DH
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature02465; PubMed=15164053 [NCBI, ExPASy, EBI, Israel, Japan]
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.;
"DNA sequence and analysis of human chromosome 9.";
Nature 429:369-374(2004).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT SER-27.
TISSUE=Cervix;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
NUCLEOTIDE SEQUENCE [MRNA] OF 1-311, AND TISSUE SPECIFICITY.
TISSUE=Kidney;
DOI=10.1074/jbc.271.5.2844; PubMed=8576264 [NCBI, ExPASy, EBI, Israel, Japan]
Yokomizo T., Ogawa Y., Uozumi N., Kume K., Izumi T., Shimizu T.;
"cDNA cloning, expression, and mutagenesis study of leukotriene B4 12-hydroxydehydrogenase.";
J. Biol. Chem. 271:2844-2850(1996).
[4]
X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 4-239.
Structural genomics consortium (SGC);
"Crystal structure of human NADP-dependent leukotriene B4 12-hydroxydehydrogenase.";
Submitted (MAY-2005) to the PDB data bank.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AL135787; CAC22151.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC035228; AAH35228.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D49387; BAA08382.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_036344.1; -.
UniGene Hs.584864
3D structure databases
PDB
1ZSV; X-ray; 2.30 A; A/B/C/D=4-329.[ExPASy / RCSB / EBI]
PDBsum 1ZSV; -.
ModBase Q14914.
PTM databases
PhosphoSite Q14914; -.
Organism-specific databases
GeneCards GC09M113365; -.
HGNC HGNC:18429; PTGR1.
GenAtlas PTGR1.
MIM 601274; gene. [NCBI / EBI]
PharmGKB PA134971979; -.
GeneCards Q14914.
Gene expression databases
ArrayExpress Q14914; -.
CleanEx HS_PTGR1; -.
GermOnline ENSG00000106853; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (non-traceable author statement from UniProtKB).
GO:0047522; Molecular function: 15-oxoprostaglandin 13-oxidase activity (inferred from electronic annotation from InterPro).
GO:0032440; Molecular function: 2-alkenal reductase activity (inferred from electronic annotation from InterPro).
GO:0004022; Molecular function: alcohol dehydrogenase activity (non-traceable author statement from UniProtKB).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from InterPro).
GO:0006691; Biological process: leukotriene metabolic process (non-traceable author statement from UniProtKB).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR002085; AlcDHase_SF_Zn.
IPR013149; AlcDHase_Zn-bd.
IPR014190; B4_12hDHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11695; ADH_Sf_Zn; 1.
Pfam PF00107; ADH_zinc_N; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR02825; B4_12hDH; 1.
Proteomics databases
PRIDE Q14914; -.
Genome annotation databases
Ensembl ENSG00000106853; Homo sapiens. [Contig view]
GeneID 22949; -.
KEGG hsa:22949; -.
Phylogenomic databases
HOGENOM Q14914; -.
HOVERGEN Q14914; -.
Other
LinkHub Q14914; -.
NextBio 43715; -.
SOURCE PTGR1; Homo sapiens.
ProtoNet Q14914.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cytoplasm; NADP; Oxidoreductase; Polymorphism.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   329  329     Prostaglandin reductase 1. PRO_0000218065
NP_BIND   149   166  18     NADP (Potential). 
COMPBIAS   250   257  8     Pro-rich. 
BINDING   178   178        NADP (By similarity). 
BINDING   193   193        NADP (By similarity). 
BINDING   217   217        NADP (By similarity). 
BINDING   245   245        NADP (By similarity). 
BINDING   321   321        NADP (By similarity). 
VARIANT   27    27  1     A -> S (in dbSNP:rs1053959 [NCBI]). VAR_023111 [3D]
CONFLICT   311   311        A -> R (in Ref. 3; BAA08382). 
STRAND   4    11  8      
STRAND   14    16  3      
HELIX   19    21  3      
STRAND   22    28  7      
STRAND   37    45  9      
HELIX   49    53  5      
HELIX   54    56  3      
STRAND   67    75  9      
STRAND   84    87  4      
STRAND   91    97  7      
HELIX   115   119  5      
TURN   120   122  3      
HELIX   124   135  12      
STRAND   144   149  6      
HELIX   155   163  9      
TURN   164   166  3      
STRAND   168   175  8      
HELIX   176   184  9      
STRAND   188   192  5      
TURN   193   195  3      
HELIX   199   206  8      
STRAND   211   218  8      
HELIX   220   227  8      
STRAND   230   238  9      
HELIX   242   244  3      
HELIX   257   262  6      
STRAND   266   269  4      
HELIX   272   274  3      
HELIX   277   292  16      
STRAND   300   304  5      
HELIX   306   308  3      
HELIX   309   317  9      
Sequence information
Length: 329 AA [This is the length of the unprocessed precursor] Molecular weight: 35870 Da [This is the MW of the unprocessed precursor] CRC64: E121ADB7C5BD9CF8 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVRTKTWTLK KHFVGYPTNS DFELKTAELP PLKNGEVLLE ALFLTVDPYM RVAAKRLKEG 

        70         80         90        100        110        120 
DTMMGQQVAK VVESKNVALP KGTIVLASPG WTTHSISDGK DLEKLLTEWP DTIPLSLALG 

       130        140        150        160        170        180 
TVGMPGLTAY FGLLEICGVK GGETVMVNAA AGAVGSVVGQ IAKLKGCKVV GAVGSDEKVA 

       190        200        210        220        230        240 
YLQKLGFDVV FNYKTVESLE ETLKKASPDG YDCYFDNVGG EFSNTVIGQM KKFGRIAICG 

       250        260        270        280        290        300 
AISTYNRTGP LPPGPPPEIV IYQELRMEAF VVYRWQGDAR QKALKDLLKW VLEGKIQYKE 

       310        320 
YIIEGFENMP AAFMGMLKGD NLGKTIVKA 

Q14914 in FASTA format

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