ID   2NPD_WILMR              Reviewed;         374 AA.
AC   Q12723;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-DEC-2008, entry version 35.
DE   RecName: Full=2-nitropropane dioxygenase;
DE            Short=2-NPD;
DE            EC=1.13.11.32;
DE   AltName: Full=Nitroalkane oxidase;
OS   Williopsis mrakii (Yeast) (Hansenula mrakii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Williopsis.
OX   NCBI_TaxID=4963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IFO 0895;
RX   MEDLINE=95112848; PubMed=7813473;
RX   DOI=10.1111/j.1432-1033.1994.00841.x;
RA   Tchorzewski M., Kurihara T., Esaki N., Soda K.;
RT   "Unique primary structure of 2-nitropropane dioxygenase from Hansenula
RT   mrakii.";
RL   Eur. J. Biochem. 226:841-846(1994).
CC   -!- FUNCTION: Catalyzes the oxygenative denitrification of various
CC       anionic nitroalkanes.
CC   -!- CATALYTIC ACTIVITY: 2 2-nitropropane + O(2) = 2 acetone + 2
CC       nitrite.
CC   -!- COFACTOR: FAD.
CC   -!- SIMILARITY: To FMN-dependent 2-nitropropane dioxygenase.
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DR   EMBL; U13900; AAA64484.1; -; Genomic_DNA.
DR   PIR; S50891; S50891.
DR   BioCyc; MetaCyc:MON-12527; -.
DR   GO; GO:0018580; F:2-nitropropane dioxygenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR004136; 2Npropane_dOase.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF03060; NPD; 1.
PE   4: Predicted;
KW   Dioxygenase; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN         1    374       2-nitropropane dioxygenase.
FT                                /FTId=PRO_0000064361.
SQ   SEQUENCE   374 AA;  41467 MW;  63F82BA7453E43F0 CRC64;
     MRSQIQSFLK TFEVRYPIIQ APMAGASTLE LAATVTRLGG IGSIPMGSLS EKCDAIETQL
     ENFDELVGDS GRIVNLNFFA HKEPRSGRAD VNEEWLKKYD KIYGKAGIEF DKKELKLLYP
     SFRSIVDPQH PTVRLLKNLK PKIVSFHFGL PHEAVIESLQ ASDIKIFVTV TNLQEFQQAY
     ESKLDGVVLQ GWEAGGHRGN FKANDVEDGQ LKTLDLVSTI VDYIDSASIS NPPFIIAAGG
     IHDDESIKEL LQFNIAAVQL GTVWLPSSQA TISPEHLKMF QSPKSDTMMT AAISGRNLRT
     ISTPFLRDLH QSSPLASIPD YPLPYDSFKS LANDAKQSGK GPQYSAFLAG SNYHKSWKDT
     RSTEEIFSIL VQDL
//