NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=IFO 0895;
DOI=10.1111/j.1432-1033.1994.00841.x; PubMed=7813473 [NCBI, ExPASy, EBI, Israel, Japan]
Tchorzewski M., Kurihara T., Esaki N., Soda K.;
"Unique primary structure of 2-nitropropane dioxygenase from Hansenula mrakii.";
Eur. J. Biochem. 226:841-846(1994).

Comments

FUNCTION: Catalyzes the oxygenative denitrification of various anionic nitroalkanes.
CATALYTIC ACTIVITY: 2 2-nitropropane + O₂ = 2 acetone + 2 nitrite.
COFACTOR: FAD.
SIMILARITY: To FMN-dependent 2-nitropropane dioxygenase.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U13900; AAA64484.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S50891; S50891.

3D structure databases

ModBase

Q12723.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-12527; -.

Ontologies

GO:0018580;	Molecular function: 2-nitropropane dioxygenase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR004136; 2Npropane_dOase.
IPR013785; Aldolase_TIM.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF03060; NPD; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; FAD; Flavoprotein; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 374`	`374`		`2-nitropropane dioxygenase.`	`PRO_0000064361`

Sequence information

Length: 374 AA [This is the length of the unprocessed precursor]

Molecular weight: 41467 Da [This is the MW of the unprocessed precursor]

CRC64: 63F82BA7453E43F0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRSQIQSFLK TFEVRYPIIQ APMAGASTLE LAATVTRLGG IGSIPMGSLS EKCDAIETQL 

        70         80         90        100        110        120 
ENFDELVGDS GRIVNLNFFA HKEPRSGRAD VNEEWLKKYD KIYGKAGIEF DKKELKLLYP 

       130        140        150        160        170        180 
SFRSIVDPQH PTVRLLKNLK PKIVSFHFGL PHEAVIESLQ ASDIKIFVTV TNLQEFQQAY 

       190        200        210        220        230        240 
ESKLDGVVLQ GWEAGGHRGN FKANDVEDGQ LKTLDLVSTI VDYIDSASIS NPPFIIAAGG 

       250        260        270        280        290        300 
IHDDESIKEL LQFNIAAVQL GTVWLPSSQA TISPEHLKMF QSPKSDTMMT AAISGRNLRT 

       310        320        330        340        350        360 
ISTPFLRDLH QSSPLASIPD YPLPYDSFKS LANDAKQSGK GPQYSAFLAG SNYHKSWKDT 

       370 
RSTEEIFSIL VQDL

Q12723 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools