ID   CP51_PENIT              Reviewed;         515 AA.
AC   Q12664;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-NOV-2008, entry version 49.
DE   RecName: Full=Cytochrome P450 51;
DE            EC=1.14.13.70;
DE   AltName: Full=CYPLI;
DE   AltName: Full=P450-LIA1;
DE   AltName: Full=Sterol 14-alpha demethylase;
DE   AltName: Full=Eburicol 14-alpha-demethylase;
DE   AltName: Full=P450-14DM;
GN   Name=CYP51;
OS   Penicillium italicum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae;
OC   mitosporic Trichocomaceae; Penicillium.
OX   NCBI_TaxID=40296;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W5;
RX   MEDLINE=96204513; PubMed=8628233; DOI=10.1007/s004380050126;
RA   van Nistelrooy J.G.M., van den Brink J.M., van Kan J.A.L.,
RA   van Gorcom R.F.M., de Waard M.A.;
RT   "Isolation and molecular characterisation of the gene encoding
RT   eburicol 14 alpha-demethylase (cYP51) from Penicillium italicum.";
RL   Mol. Gen. Genet. 250:725-733(1996).
CC   -!- CATALYTIC ACTIVITY: Obtusifoliol + 3 O(2) + 3 NADPH = 4-alpha-
CC       methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3
CC       NADP(+) + 4 H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane (Potential).
CC       Microsome membrane (Potential).
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; Z49750; CAA89824.1; -; Genomic_DNA.
DR   PIR; S65578; S65578.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005792; C:microsome; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0008398; F:sterol 14-demethylase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00465; EP450IV.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Heme; Iron; Lipid synthesis; Membrane;
KW   Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase;
KW   Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
FT   CHAIN         1    515       Cytochrome P450 51.
FT                                /FTId=PRO_0000052008.
FT   TRANSMEM     11     31       Potential.
FT   METAL       459    459       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   515 AA;  58137 MW;  13679B56F8769255 CRC64;
     MDLVPLVTGQ ILGIAYYTTG LFLVSIVLNV IKQLIFYNRK EPPVVFHWIP FIGSTIAYGM
     DPYQFFFASR AKYGDIFTFI LLGKKTTVYL GVEGNEFILN GKLKDVNAEE VYGKLTTPVF
     GSDVVYDCPN SKLMEQKKFI KYGLSQEALE SYVPLIADET NAYIKSSPNF KGQSGTIDLA
     AAMAEITIFT AARTLQGEEV RSKLTSEFAD LFHDLDLGFS PINFMLPWAP LPHNASAIKH
     TTYARDLSGN YPSATGSWRR RQRRRQDKSK GTDMISNLMR CVYRDGTPIP DKEIAHMMIT
     LLMAGQHSSS AISCWILLRL ASQPEMAEKL HAEQIKNLGA DLPPLQYKDM DKLPLLRNVI
     KETLRLHSSI HTLMRKVKNP MPVPGTDFVV PPSHTLLSSP GVTARDERHF RDPLRWDPHR
     WESRVEVEDS SDTVDYGYGA VSKGTRSPYL PFGAGRHRCI GEKFAYLNLE VIVATLVREF
     RFFNPEGMEG VPDTDYSSLF SRPVQPATVR WEVRS
//