NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=W5;
DOI=10.1007/s004380050126; PubMed=8628233 [NCBI, ExPASy, EBI, Israel, Japan]
van Nistelrooy J.G.M., van den Brink J.M., van Kan J.A.L., van Gorcom R.F.M., de Waard M.A.;
"Isolation and molecular characterisation of the gene encoding eburicol 14 alpha-demethylase (cYP51) from Penicillium italicum.";
Mol. Gen. Genet. 250:725-733(1996).

Comments

CATALYTIC ACTIVITY: Obtusifoliol + 3 O₂ + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24²⁸-trien-3-beta-ol + formate + 3 NADP⁺ + 4 H₂O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane (Potential). Microsome membrane (Potential).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z49750; CAA89824.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S65578; S65578.

3D structure databases

ModBase

Q12664.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00465; EP450IV.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Heme; Iron; Lipid synthesis; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 515`	`515`	`Cytochrome P450 51.`	`PRO_0000052008`
`TRANSMEM`	`11 31`	`21`	`Potential.`
`METAL`	`459 459`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 515 AA [This is the length of the unprocessed precursor]

Molecular weight: 58137 Da [This is the MW of the unprocessed precursor]

CRC64: 13679B56F8769255 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDLVPLVTGQ ILGIAYYTTG LFLVSIVLNV IKQLIFYNRK EPPVVFHWIP FIGSTIAYGM 

        70         80         90        100        110        120 
DPYQFFFASR AKYGDIFTFI LLGKKTTVYL GVEGNEFILN GKLKDVNAEE VYGKLTTPVF 

       130        140        150        160        170        180 
GSDVVYDCPN SKLMEQKKFI KYGLSQEALE SYVPLIADET NAYIKSSPNF KGQSGTIDLA 

       190        200        210        220        230        240 
AAMAEITIFT AARTLQGEEV RSKLTSEFAD LFHDLDLGFS PINFMLPWAP LPHNASAIKH 

       250        260        270        280        290        300 
TTYARDLSGN YPSATGSWRR RQRRRQDKSK GTDMISNLMR CVYRDGTPIP DKEIAHMMIT 

       310        320        330        340        350        360 
LLMAGQHSSS AISCWILLRL ASQPEMAEKL HAEQIKNLGA DLPPLQYKDM DKLPLLRNVI 

       370        380        390        400        410        420 
KETLRLHSSI HTLMRKVKNP MPVPGTDFVV PPSHTLLSSP GVTARDERHF RDPLRWDPHR 

       430        440        450        460        470        480 
WESRVEVEDS SDTVDYGYGA VSKGTRSPYL PFGAGRHRCI GEKFAYLNLE VIVATLVREF 

       490        500        510 
RFFNPEGMEG VPDTDYSSLF SRPVQPATVR WEVRS

Q12664 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools