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UniProtKB/Swiss-Prot entry Q12634


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name T4HR_MAGGR
Primary accession number Q12634
Secondary accession numbers A4R3G6 Q5I7G0
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 68)
Name and origin of the protein
Protein name Tetrahydroxynaphthalene reductase
Synonyms EC 1.1.1.252
T4HN reductase
THNR
Gene name
ORFNames: MGG_02252
From
Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [TaxID: 148305] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Sordariomycetes incertae sedis; Magnaporthaceae; Magnaporthe.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
STRAIN=Guyane 11;
PubMed=8112349 [NCBI, ExPASy, EBI, Israel, Japan]
Vidal-Cros A., Viviani F., Labesse G., Boccara M., Gaudry M.;
"Polyhydroxynaphthalene reductase involved in melanin biosynthesis in Magnaporthe grisea. Purification, cDNA cloning and sequencing.";
Eur. J. Biochem. 219:985-992(1994).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1111/j.1439-0434.2006.01111.x; AGRICOLA=IND43821857
Zhang C.-Q., Zhu G.N., Ma Z.H., Zhou M.-G.;
"Isolation, characterization and preliminary genetic analysis of laboratory tricyclazole-resistant mutants of the rice blast fungus, Magnaporthe grisea.";
J. Phytopathol. 154:392-397(2006).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=70-15 / FGSC 8958;
DOI=10.1038/nature03449; PubMed=15846337 [NCBI, ExPASy, EBI, Israel, Japan]
Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
"The genome sequence of the rice blast fungus Magnaporthe grisea.";
Nature 434:980-986(2005).
[4]
X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NADP AND INHIBITOR.
STRAIN=4091-5-8;
DOI=10.1016/S0969-2126(96)00124-4; PubMed=8939741 [NCBI, ExPASy, EBI, Israel, Japan]
Andersson A., Jordan D.B., Schneider G., Lindqvist Y.;
"Crystal structure of the ternary complex of 1,3,8-trihydroxynaphthalene reductase from Magnaporthe grisea with NADPH and an active-site inhibitor.";
Structure 4:1161-1170(1996).
[5]
X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH NADP AND FUNGICIDE.
DOI=10.1016/S0969-2126(00)00548-7; PubMed=11342131 [NCBI, ExPASy, EBI, Israel, Japan]
Liao D.-I., Basarab G.S., Gatenby A.A., Valent B., Jordan D.B.;
"Structures of trihydroxynaphthalene reductase-fungicide complexes: implications for structure-based design and catalysis.";
Structure 9:19-27(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L22309; AAA19514.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY846878; AAW55623.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CH476830; EDJ98831.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S41412; S41412.
RefSeq XP_365550.1; -.
UniGene Mgr.5801
3D structure databases
PDB
1DOH; X-ray; 2.10 A; A/B=1-283.[ExPASy / RCSB / EBI]
1G0N; X-ray; 2.00 A; A/B=1-283.[ExPASy / RCSB / EBI]
1G0O; X-ray; 1.70 A; A/B/C/D=1-283.[ExPASy / RCSB / EBI]
1YBV; X-ray; 2.80 A; A/B=3-283.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1DOH; -.
1G0N; -.
1G0O; -.
1YBV; -.
ModBase Q12634.
Ontologies
GO
GO:0005488; Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0047039; Molecular function: tetrahydroxynaphthalene reductase activity (inferred from electronic annotation from EC).
GO:0006583; Biological process: melanin biosynthetic process from tyrosine (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR19410; ADH_short_C2; 1.
Pfam PF00106; adh_short; 1.
Pfam graphical view of domain structure.
PRINTS PR00081; GDHRDH.
PR00080; SDRFAMILY.
PROSITE PS00061; ADH_SHORT; 1.
Genome annotation databases
GeneID 2681349; -.
KEGG mgr:MGG_02252; -.
NMPDR fig|242507.1.peg.7289; -.
Other
LinkHub Q12634; -.
ProtoNet Q12634.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Direct protein sequencing; Melanin biosynthesis; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   283  282     Tetrahydroxynaphthalene reductase. PRO_0000054782
NP_BIND   39    63  25     NADP. 
ACT_SITE   178   178        Proton acceptor. 
BINDING   164   164        Substrate. 
HELIX   12    14  3      
HELIX   21    25  5      
STRAND   31    34  4      
TURN   35    38  4      
HELIX   40    51  12      
STRAND   55    62  8      
HELIX   64    76  13      
STRAND   81    85  5      
HELIX   91   105  15      
STRAND   108   113  6      
HELIX   123   125  3      
HELIX   128   138  11      
HELIX   140   152  13      
STRAND   153   162  10      
HELIX   165   167  3      
HELIX   176   195  20      
HELIX   196   199  4      
STRAND   202   208  7      
HELIX   214   219  6      
HELIX   220   223  4      
HELIX   232   242  11      
HELIX   252   263  12      
HELIX   265   267  3      
STRAND   274   280  7      
Sequence information
Length: 283 AA [This is the length of the unprocessed precursor] Molecular weight: 30054 Da [This is the MW of the unprocessed precursor] CRC64: E079638A96DF19CA [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPAVTQPRGE SKYDAIPGPL GPQSASLEGK VALVTGAGRG IGREMAMELG RRGCKVIVNY 

        70         80         90        100        110        120 
ANSTESAEEV VAAIKKNGSD AACVKANVGV VEDIVRMFEE AVKIFGKLDI VCSNSGVVSF 

       130        140        150        160        170        180 
GHVKDVTPEE FDRVFTINTR GQFFVAREAY KHLEIGGRLI LMGSITGQAK AVPKHAVYSG 

       190        200        210        220        230        240 
SKGAIETFAR CMAIDMADKK ITVNVVAPGG IKTDMYHAVC REYIPNGENL SNEEVDEYAA 

       250        260        270        280 
SAWSPLHRVG LPIDIARVVC FLASNDGGWV TGKVIGIDGG ACM 

Q12634 in FASTA format

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View entry in raw text format (no links)
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