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UniProtKB/Swiss-Prot entry Q12493


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NKP1_YEAST
Primary accession number Q12493
Secondary accession numbers None
Integrated into Swiss-Prot on September 13, 2005
Sequence was last modified on September 13, 2005 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 51)
Name and origin of the protein
Protein name Central kinetochore subunit NKP1
Synonym Non-essential kinetochore protein 1
Gene name
Name: NKP1
OrderedLocusNames: YDR383C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169867 [NCBI, ExPASy, EBI, Israel, Japan]
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
Nature 387:75-78(1997).
[2]
IDENTIFICATION OF PROBABLE INITIATION SITE.
DOI=10.1038/nature01644; PubMed=12748633 [NCBI, ExPASy, EBI, Israel, Japan]
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
"Sequencing and comparison of yeast species to identify genes and regulatory elements.";
Nature 423:241-254(2003).
[3]
IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CENTRAL KINETOCHORE COMPLEX, AND SUBCELLULAR LOCATION.
DOI=10.1016/S0092-8674(02)00973-X; PubMed=12408861 [NCBI, ExPASy, EBI, Israel, Japan]
Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.;
"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p.";
Cell 111:163-172(2002).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-222, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
  • FUNCTION: Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.
  • SUBUNIT: Component of the central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.
  • INTERACTION:
    Q06162:NKP2; NbExp=1; IntAct=EBI-35840, EBI-34256;
    Q02821:SRP1; NbExp=1; IntAct=EBI-35840, EBI-1797;
  • SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with kinetochores.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U28373; AAB64819.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U32274; AAB64825.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S61178; S61178.
RefSeq NP_010671.2; -.
3D structure databases
ModBase Q12493.
Protein-protein interaction databases
DIP DIP:1940N; -.
IntAct Q12493; 16.
Organism-specific databases
CYGD YDR383c; -.
SGD S000002791; NKP1.
Yeast-GFP YDR383C.
Gene expression databases
GermOnline YDR383C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0000777; Cellular component: condensed chromosome kinetochore (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0051301; Biological process: cell division (inferred from electronic annotation from UniProtKB-KW).
GO:0007126; Biological process: meiosis (inferred from electronic annotation from UniProtKB-KW).
GO:0007067; Biological process: mitosis (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Genome annotation databases
Ensembl YDR383C; Saccharomyces cerevisiae. [Contig view]
GeneID 851991; -.
GenomeReviews Z71256_GR; YDR383C.
KEGG sce:YDR383C; -.
NMPDR fig|4932.3.peg.1443; -.
Phylogenomic databases
HOGENOM Q12493; -.
Other
LinkHub Q12493; -.
NextBio 970157; -.
ProtoNet Q12493.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell cycle; Cell division; Complete proteome; Kinetochore; Meiosis; Mitosis; Nucleus; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   238  238     Central kinetochore subunit NKP1. PRO_0000096867
MOD_RES   222   222        Phosphoserine. 
Sequence information
Length: 238 AA [This is the length of the unprocessed precursor] Molecular weight: 26976 Da [This is the MW of the unprocessed precursor] CRC64: A2BE651F72E76B54 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTDTYNSISN FIENELTALL SSDDYLMDDL AGELPNEVCR LLKAQVIEKR KDAMSRGKQD 

        70         80         90        100        110        120 
LLSKEIYDNE SELRASQSQQ IMELVGDIPK YSLGSELRNR VEGEPQSTSI ERLIEDVLKL 

       130        140        150        160        170        180 
PQMEVADEEE VEVENDLKVL SEYSNLRKDL ILKCQALQIG ESKLSDILSQ TNSINSLTTS 

       190        200        210        220        230 
IKEASEDDDI SEYFATYNGK LVVALEEMKL LLEEAVKTFG NSPEKREKIK KILSELKK 

Q12493 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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