FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis (light-independent).
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000393; ABG50160.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_720633.1; -.

3D structure databases

ModBase

Q118B4.

Enzyme and pathway databases

BioCyc

TERY203124:TERY_0728-MON; -.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

Q118B4.

Genome annotation databases

GeneID

4243176; -.

GenomeReviews

CP000393_GR; Tery_0728.

KEGG

ter:Tery_0728; -.

NMPDR

fig|203124.1.peg.687; -.

Phylogenomic databases

HOGENOM

Q118B4; -.

Genome annotation databases

CMR

Q118B4; Tery_0728.

Other

ProtoNet

Q118B4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 358`	`358`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_1000070557`

Sequence information

Length: 358 AA [This is the length of the unprocessed precursor]

Molecular weight: 42736 Da [This is the MW of the unprocessed precursor]

CRC64: BB0CF4E843525765 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVNSLKKQTF TEMRPGVKVP AKETILTPRF YTTDFDEMAK MDISVNEDEL MAILEEFRAD 

        70         80         90        100        110        120 
YNRHHFVRNE EFAQSWDHID GETRRLFVEF LERSCTAEFS GFLLYKELGR RLKNKSPILA 

       130        140        150        160        170        180 
ECFTLMSRDE ARHAGFLNKA MSDFNLSLDL GFLTKSRKYT FFKPKFIFYA TYLSEKIGYW 

       190        200        210        220        230        240 
RYITIYRHLE AHPEDRIYPI FRFFENWCQD ENRHGDFFDA IMRARPEFLN DWQAKLWCRF 

       250        260        270        280        290        300 
FLLSVFATMY LNDIQRADFY ASIGLNACDY DKYVIEKTNE TSGRVFPVIL DVENPEFYAR 

       310        320        330        340        350 
LEFCIKNNEK LTKIANSNNP GFVKFFQKFP LYLSNGWQFL KLYLMKPIET ATMQSSVR

Q118B4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools