ID   HCAF_SHIF8              Reviewed;         172 AA.
AC   Q0T1Y0;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   04-NOV-2008, entry version 20.
DE   RecName: Full=3-phenylpropionate/cinnamic acid dioxygenase subunit beta;
DE            EC=1.14.12.19;
DE   AltName: Full=Digoxigenin subunit beta;
GN   Name=hcaF; OrderedLocusNames=SFV_2587;
OS   Shigella flexneri serotype 5b (strain 8401).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=373384;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16822325; DOI=10.1186/1471-2164-7-173;
RA   Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z.,
RA   Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y.,
RA   Jin Q.;
RT   "Complete genome sequence of Shigella flexneri 5b and comparison with
RT   Shigella flexneri 2a.";
RL   BMC Genomics 7:173-173(2006).
CC   -!- FUNCTION: Part of the multicomponent 3-phenylpropionate
CC       dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic
CC       acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC       cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: 3-phenylpropanoic acid + NADH + O(2) = cis-3-
CC       (2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD(+).
CC   -!- CATALYTIC ACTIVITY: Cinnamic acid + H(+) + NADH + O(2) = cis-3-(2-
CC       carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD(+).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid
CC       degradation.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the
CC       two subunits of the hydroxylase component (hcaE and hcaF), a
CC       ferredoxin (hcaC) and a ferredoxin reductase (hcaD) (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase beta subunit family.
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DR   EMBL; CP000266; ABF04685.1; -; Genomic_DNA.
DR   RefSeq; YP_689990.1; -.
DR   GeneID; 4207322; -.
DR   GenomeReviews; CP000266_GR; SFV_2587.
DR   KEGG; sfv:SFV_2587; -.
DR   HOGENOM; Q0T1Y0; -.
DR   BioCyc; SFLE373384:SFV_2587-MON; -.
DR   GO; GO:0008695; F:3-phenylpropionate dioxygenase activity; IEA:HAMAP.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01649; -; 1.
DR   InterPro; IPR000391; Rng_hydr_dOase-B.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase; NAD;
KW   Oxidoreductase.
FT   CHAIN         1    172       3-phenylpropionate/cinnamic acid
FT                                dioxygenase subunit beta.
FT                                /FTId=PRO_0000333716.
SQ   SEQUENCE   172 AA;  20613 MW;  582027F378D97554 CRC64;
     MSAQVSLELH HRISQFLFHE ASLLDDWKFR DWLAQLDEEI RYTMRTTVNA QTRDRRKGVQ
     PPTTWIFNDT KDQLERRIAR LETGMAWAEE PPSRTRHLIS NCQVSETDIP NVFAVRVNYL
     LYRAQKERDE TFYVGTRFDK VRRLEYDNWR LLERDIVLDQ AVITSHNLSV LF
//