ID   MAO1_SHEFN              Reviewed;         562 AA.
AC   Q086X9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   25-NOV-2008, entry version 24.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=Sfri_0833;
OS   Shewanella frigidimarina (strain NCIMB 400).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=318167;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T.,
RA   Nealson K.H., Newman D., Tiedje J.M., Zhou J., Romine M.F.,
RA   Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C.,
RA   Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Mikhailova N., Richardson P.;
RT   "Complete sequence of Shewanella frigidimarina NCIMB 400.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000447; ABI70686.1; -; Genomic_DNA.
DR   RefSeq; YP_749524.1; -.
DR   GeneID; 4276930; -.
DR   GenomeReviews; CP000447_GR; Sfri_0833.
DR   KEGG; sfr:Sfri_0833; -.
DR   NMPDR; fig|318167.10.peg.792; -.
DR   HOGENOM; Q086X9; -.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    562       NAD-dependent malic enzyme.
FT                                /FTId=PRO_1000069542.
FT   ACT_SITE    101    101       Proton donor (By similarity).
FT   ACT_SITE    172    172       Proton acceptor (By similarity).
FT   METAL       243    243       Divalent metal cation (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       267    267       Divalent metal cation (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     415    415       NAD (By similarity).
FT   SITE        267    267       Important for activity (By similarity).
SQ   SEQUENCE   562 AA;  62258 MW;  B19107A9328466BA CRC64;
     MDDNKRPLYL PFAGPAILES PLLNKGSAFS EEERIYFNLE GLIPWVIETI DEQAARAYAQ
     FKNFTNDLDK HIYLRNIQDT NETLFYRLVR NNISEMMPII YTPTVGLACE RFSKNYRRNR
     GLFISYPNKD RIDDILNNST RHKVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG
     GISPAYCLAV TLDVGTDNPQ LLEDPMYMGW RHQRIGGDEY AEFVEEFMQA VSRRWPDALI
     QFEDFAQKNA MPLLERYKDQ YCSFNDDIQG TAAVTVGSLL AACKAANSKL SEQRITFLGA
     GSAGCGIAEA IVATMVSEGI SDQQARSQVF MVDRWGLLLD NMPNLLPFQQ KLAQPCATIE
     AWDNYSDNIS LLDVVNNAKP TVLIGVSGSP GLFTEEIIKA MHSHCKRPIV FPLSNPTSRV
     EATPKDILNW TSGQALVATG SPFEPVIVNG ETFEIAQCNN SFIFPGIGLG VLSCGARRVS
     DEMLMASSRA LAECSPLGKD GVGSLLPRLE DIQTVSKYIA FAVAKAAIDQ GLALPCTDEL
     LQQSIEANFW EPEYRRYKRT SF
//