NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=cv. Blue Titan;
TISSUE=Flower bud;
PubMed=1544919 [NCBI, ExPASy, EBI, Israel, Japan]
Britsch L., Ruhnau-Brich B., Forkmann G.;
"Molecular cloning, sequence analysis, and in vitro expression of flavanone 3 beta-hydroxylase from Petunia hybrida.";
J. Biol. Chem. 267:5380-5387(1992).

Comments

FUNCTION: Catalyzes the 3-beta-hydroxylation of 2S-flavonones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O₂ = a dihydroflavonol + succinate + CO₂.
COFACTOR: Iron.
COFACTOR: Ascorbate.
PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis .
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X60512; CAA43027.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A42110; A42110.
S16780; S16780.

3D structure databases

HSSP

Q96323; 1GP6. [HSSP ENTRY / PDB]

ModBase

Q07353.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-11838; -.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Direct protein sequencing; Flavonoid biosynthesis; Iron; Metal-binding; Oxidoreductase; Vitamin C.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 369`	`>369`	`Naringenin,2-oxoglutarate 3-dioxygenase.`	`PRO_0000067289`
`METAL`	`78 78`		`Iron (Potential).`
`METAL`	`220 220`		`Iron (Potential).`
`METAL`	`222 222`		`Iron (Potential).`
`METAL`	`278 278`		`Iron (Potential).`
`NON_TER`	`1 1`

Sequence information

Length: 369 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 41466 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: C34034E23B4D5A3F [This is a checksum on the sequence]

        10         20         30         40         50         60 
IPRVTPSTLT ALAEEKTLQT SFIRDEDERP KVAYNQFSNE IPIISLEGID DETGKRAEIC 

        70         80         90        100        110        120 
DKIVKACEDW GVFQVVDHGV DAEVISQMTT FAKEFFALPP EEKLRFDMSG GKKGGFIVSS 

       130        140        150        160        170        180 
HLQGEVVQDW REIVTYFSYP TRARDYSRWP DKPEGWIAVT QKYSEKLMEL ACKLLDVLSE 

       190        200        210        220        230        240 
AMGLEKEALT KACVDMDQKV VVNFYPKCPE PDLTLGLKRH TDPGTITLLL QDQVGGLQAT 

       250        260        270        280        290        300 
KDNGKTWITV QPVEGAFVVN LGDHGHFLSN GRFKNADHQA VVNSNSSRLS IATFQNPAPE 

       310        320        330        340        350        360 
AIVYPLKIRE GEKSIMDEPI TFAEMYRRKM SKDLELARLK KQAKEQQLQA EVAAEKAKLE 


SKPIEEILA

Q07353 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools