[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Leaf; DOI=10.1104/pp.103.1.291; PubMed=8208851 [NCBI, ExPASy, EBI, Israel, Japan] Davies K.M.; "A cDNA clone for flavanone 3-hydroxylase from Malus."; Plant Physiol. 103:291-291(1993).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. Fuji; TISSUE=Peelings; Lee J.-R., Hong S.-T., Yoo Y.G., Kim S.-R.; "Molecular cloning and expression of anthocyanin biosynthesis genes from 'Fuji apple'."; Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the 3-beta-hydroxylation of 2S-flavonones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O₂ = a dihydroflavonol + succinate + CO₂.
COFACTOR: Iron.
COFACTOR: Ascorbate.
PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis .
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X69664; CAA49353.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF117270; AAD26206.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S31458; S31458.

3D structure databases

ModBase

Q06942.

Ontologies

GO:0045486;	Molecular function: naringenin 3-dioxygenase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding; Oxidoreductase; Vitamin C.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 364`	`364`	`Naringenin,2-oxoglutarate 3-dioxygenase.`	`PRO_0000067287`
`METAL`	`76 76`		`Iron (Potential).`
`METAL`	`218 218`		`Iron (Potential).`
`METAL`	`220 220`		`Iron (Potential).`
`METAL`	`276 276`		`Iron (Potential).`

Sequence information

Length: 364 AA [This is the length of the unprocessed precursor]

Molecular weight: 40771 Da [This is the MW of the unprocessed precursor]

CRC64: B84793FCF831CE44 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAPATTLTSI AHEKTLQQKF VRDEDERPKV AYNDFSNEIP IISLAGIDEV EGRRGEICKK 

        70         80         90        100        110        120 
IVAACEDWGI FQIVDHGVDA ELISEMTGLA REFFALPSEE KLRFDMSGGK KGGFIVSSHL 

       130        140        150        160        170        180 
QGEAVQDWRE IVTYFSYPIR HRDYSRWPDK PEAWREVTKK YSDELMGLAC KLLGVLSEAM 

       190        200        210        220        230        240 
GLDTEALTKA CVDMDQKVVV NFYPKCPQPD LTLGLKRHTD PGTITLLLQD QVGGLQATRD 

       250        260        270        280        290        300 
DGKTWITVQP VEGAFVVNLG DHGHLLSNGR FKNADHQAVV NSNSSRLSIA TFQNPAQEAI 

       310        320        330        340        350        360 
VYPLSVREGE KPILEAPITY TEMYKKKMSK DLELARLKKL AKEQQSQDLE KAKVDTKPVD 


DIFA

Q06942 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools