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UniProtKB/Swiss-Prot entry Q06215

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PPO_VICFA
Primary accession number Q06215
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1995
Sequence was last modified on November 1, 1995 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 48)
Name and origin of the protein
Protein name Polyphenol oxidase A1, chloroplastic [Precursor]
Synonyms PPO
EC 1.10.3.1
Catechol oxidase
Gene name None
From
Vicia faba (Broad bean) [TaxID: 3906] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Vicieae; Vicia.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
TISSUE=Leaf;
DOI=10.1007/BF00014492; PubMed=1391768 [NCBI, ExPASy, EBI, Israel, Japan]
Cary J.W., Lax A.R., Flurkey W.H.;
"Cloning and characterization of cDNAs coding for Vicia faba polyphenol oxidase.";
Plant Mol. Biol. 20:245-253(1992).
[2]
 PRELIMINARY PROTEIN SEQUENCE OF 93-119.
TISSUE=Leaf;
PubMed=16667057 [NCBI, ExPASy, EBI, Israel, Japan]
Flurkey W.H.;
"Polypeptide composition and amino-terminal sequence of broad bean polyphenol oxidase.";
Plant Physiol. 91:481-483(1989).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z11702; CAA77764.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S24758; S24758.
3D structure databases
ModBase Q06215.
Ontologies
GO
GO:0004097; Molecular function: catechol oxidase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR008922; Di-copper_centre.
IPR016213; Polyphenol_Oxase_pln.
IPR002227; Tyrosinase.
Graphical view of domain structure.
Gene3D G3DSA:1.10.1280.10; Di-copper_centre; 1.
Pfam PF00264; Tyrosinase; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000290; PPO_plant; 1.
PRINTS PR00092; TYROSINASE.
PROSITE PS00497; TYROSINASE_1; 1.
PS00498; TYROSINASE_2; 1.
BLOCKS Q06215.
Other
ProtoNet Q06215.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Chloroplast; Copper; Direct protein sequencing; Metal-binding; Oxidoreductase; Plastid; Thioether bond; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    92  92     Chloroplast. 
CHAIN   93   606  514     Polyphenol oxidase A1, chloroplastic. PRO_0000035919
METAL   181   181        Copper A (By similarity). 
METAL   202   202        Copper A (By similarity). 
METAL   211   211        Copper A (By similarity). 
METAL   333   333        Copper B (By similarity). 
METAL   337   337        Copper B (By similarity). 
METAL   367   367        Copper B (By similarity). 
DISULFID   103   121        By similarity. 
DISULFID   120   182        By similarity. 
CROSSLNK   185   202        2'-(S-cysteinyl)-histidine (Cys-His) (By similarity). 
Sequence information
Length: 606 AA [This is the length of the unprocessed precursor] Molecular weight: 68541 Da [This is the MW of the unprocessed precursor] CRC64: FC539F9F041B503C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTSISALSFI STINVSSNSK ISHSSVYPFL QKQHQSSKLR KPKRQVTCSS NNNQNNPKEE 

        70         80         90        100        110        120 
QELSNIVGHR RNVLIGLGGI YGTLATNPSA LASPISPPDL SKCVPPSDLP SGTTPPNINC 

       130        140        150        160        170        180 
CPPYSTKITD FKFPSNQPLR VRQAAHLVDN EFLEKYKKAT ELMKALPSND PRNFTQQANI 

       190        200        210        220        230        240 
HCAYCDGAYS QIGFPDLKLQ VHGSWLFFPF HRWYLYFYER ILGSLINDPT FALPFWNYDA 

       250        260        270        280        290        300 
PDGMQLPTIY ADKASPLYDE LRNASHQPPT LIDLNFCDIG SDIDRNELIK TNLSIMYRQV 

       310        320        330        340        350        360 
YSNGKTSRLF LGNPYRAGDA EPQGAGSIEN VPHAPVHTWT GDNTQTNIED MGIFYSAARD 

       370        380        390        400        410        420 
PIFYSHHSNV DRLWYIWKTL GGKKHDFTDK DWLESGFLFY DENKNLVRVN VKDSLDIDKL 

       430        440        450        460        470        480 
GYAYQDVPIP WEKAKPVPRR TKVQKLVEVE VNDGNLRKSP TIFLVRQQSP RKYVTFPLVL 

       490        500        510        520        530        540 
NNKVSAIVKR PKKLRSKKEK EEEEEVLVIE GIEFYMNIAI KFDVYINDED DKVGAGNTEF 

       550        560        570        580        590        600 
AGSFVNIPHS AHGHKNKKII TSLRLGITDL LEDLHVEGDD NIVVTLVPKC GSGQVKINNV 


EIVFED
Q06215 in FASTA format

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