NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Flower bud;
PubMed=8223617 [NCBI, ExPASy, EBI, Israel, Japan]
Britsch L., Dedio J., Saedler H., Forkmann G.;
"Molecular characterization of flavanone 3 beta-hydroxylases. Consensus sequence, comparison with related enzymes and the role of conserved histidine residues.";
Eur. J. Biochem. 217:745-754(1993).

Comments

FUNCTION: Catalyzes the 3-beta-hydroxylation of 2S-flavonones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O₂ = a dihydroflavonol + succinate + CO₂.
COFACTOR: Iron.
COFACTOR: Ascorbate.
PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis .
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X72593; CAA51191.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S38336; S32147.

3D structure databases

HSSP

Q96323; 1GP4. [HSSP ENTRY / PDB]

ModBase

Q05963.

Ontologies

GO:0045486;	Molecular function: naringenin 3-dioxygenase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding; Oxidoreductase; Vitamin C.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 356`	`356`	`Naringenin,2-oxoglutarate 3-dioxygenase.`	`PRO_0000067284`
`METAL`	`73 73`		`Iron (Potential).`
`METAL`	`215 215`		`Iron (Potential).`
`METAL`	`217 217`		`Iron (Potential).`
`METAL`	`273 273`		`Iron (Potential).`

Sequence information

Length: 356 AA [This is the length of the unprocessed precursor]

Molecular weight: 40210 Da [This is the MW of the unprocessed precursor]

CRC64: BE622A4A8C39BA0B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAPISLKWE EHSLHENKFV RDEDERPKVP YNTFSNEIPV ISLAGIDGCR RAEICDEIVK 

        70         80         90        100        110        120 
ACEDWGIFQV VDHGVDTKLL SDMTGLARDF FHLPTQEKLR FDMTGGKKGG FIVSSHLQGE 

       130        140        150        160        170        180 
AVQDWREIVT YFSYPIKARD YSRWPDKPNE WRAVTEEYSK VLMGLACKLL EVLSEAMGLE 

       190        200        210        220        230        240 
KEALTKACVD MDQKVVVNYY PKCPQPDLTL GLKRHTDPGT ITLLLQDQVG GLQATRDGGE 

       250        260        270        280        290        300 
SWITVKPVEG AFVVNLGDHG HYLSNGRFKN ADHQAVVNSS TSRLSIATFQ NPAPEAIVYP 

       310        320        330        340        350 
LKINEGEKSI MEEPMTFMEM YKKKMSTDLE LARLKKLAKD KQQDLEVVKP IQNIFA

Q05963 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools