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UniProtKB/Swiss-Prot entry Q05047


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name C72A1_CATRO
Primary accession number Q05047
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1994
Sequence was last modified on February 1, 1994 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 64)
Name and origin of the protein
Protein name Secologanin synthase
Synonyms SLS
EC 1.3.3.9
Cytochrome P450 72A1
CYPLXXII
Gene name
Name: CYP72A1
Synonyms: CYP72, P450CR3
From
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea) [TaxID: 4058] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae; Catharanthus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. CP3A;
PubMed=16653087 [NCBI, ExPASy, EBI, Israel, Japan]
Vetter H.-P., Mangold U., Schroeder G., Marner F.-J., Werck-Reichhart D., Schroeder J.;
"Molecular analysis and heterologous expression of an inducible cytochrome P-450 protein from periwinkle (Catharanthus roseus L.).";
Plant Physiol. 100:998-1007(1992).
[2]
NUCLEOTIDE SEQUENCE [MRNA] OF 469-524.
STRAIN=cv. G. Don;
DOI=10.1007/BF00014944; PubMed=8507838 [NCBI, ExPASy, EBI, Israel, Japan]
Meijer A.H., Souer E., Verpoorte R., Hoge J.H.C.;
"Isolation of cytochrome P-450 cDNA clones from the higher plant Catharanthus roseus by a PCR strategy.";
Plant Mol. Biol. 22:379-383(1993).
[3]
FUNCTION.
STRAIN=cv. CP3A;
PubMed=11135113 [NCBI, ExPASy, EBI, Israel, Japan]
Irmler S., Schroeder G., St Pierre B., Crouch N.P., Hotze M., Schmidt J., Strack D., Matern U., Schroeder J.;
"Indole alkaloid biosynthesis in Catharanthus roseus: new enzyme activities and identification of cytochrome P450 CYP72A1 as secologanin synthase.";
Plant J. 24:797-804(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L10081; AAA33106.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X69775; CAA49430.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S35168; S35168.
T09944; T09944.
3D structure databases
HSSP P14779; 1JPZ. [HSSP ENTRY / PDB]
ModBase Q05047.
Ontologies
GO
GO:0005774; Cellular component: vacuolar membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00463; EP450I.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS Q05047.
Other
ProtoNet Q05047.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alkaloid metabolism; Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Transmembrane; Vacuole.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   524  524     Secologanin synthase. PRO_0000052124
TRANSMEM   12    32  21     Potential. 
METAL   470   470        Iron (heme axial ligand) (By similarity). 
VARIANT   190   190  1     I -> L. 
VARIANT   194   194  1     Q -> E. 
VARIANT   223   223  1     E -> D. 
VARIANT   312   312  1     K -> R. 
VARIANT   318   318  1     S -> T. 
VARIANT   403   403  1     V -> I. 
VARIANT   405   405  1     K -> E. 
VARIANT   411   411  1     S -> P. 
Sequence information
Length: 524 AA [This is the length of the unprocessed precursor] Molecular weight: 60558 Da [This is the MW of the unprocessed precursor] CRC64: EF5D864E43C751E8 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEMDMDTIRK AIAATIFALV MAWAWRVLDW AWFTPKRIEK RLRQQGFRGN PYRFLVGDVK 

        70         80         90        100        110        120 
ESGKMHQEAL SKPMEFNNDI VPRLMPHINH TINTYGRNSF TWMGRIPRIH VMEPELIKEV 

       130        140        150        160        170        180 
LTHSSKYQKN FDVHNPLVKF LLTGVGSFEG AKWSKHRRII SPAFTLEKLK SMLPAFAICY 

       190        200        210        220        230        240 
HDMLTKWEKI AEKQGSHEVD IFPTFDVLTS DVISKVAFGS TYEEGGKIFR LLKELMDLTI 

       250        260        270        280        290        300 
DCMRDVYIPG WSYLPTKRNK RMKEINKEIT DMLRFIINKR MKALKAGEPG EDDLLGVLLE 

       310        320        330        340        350        360 
SNIQEIQKQG NKKDGGMSIN DVIEECKLFY FAGQETTGVL LTWTTILLSK HPEWQERARE 

       370        380        390        400        410        420 
EVLQAFGKNK PEFERLNHLK YVSMILYEVL RLYPPVIDLT KIVHKDTKLG SYTIPAGTQV 

       430        440        450        460        470        480 
MLPTVMLHRE KSIWGEDAME FNPMRFVDGV ANATKNNVTY LPFSWGPRVC LGQNFALLQA 

       490        500        510        520 
KLGLAMILQR FKFDVAPSYV HAPFTILTVQ PQFGSHVIYK KLES 

Q05047 in FASTA format

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