ID   HMDH_CATRO              Reviewed;         601 AA.
AC   Q03163;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-NOV-2008, entry version 64.
DE   RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase;
DE            Short=HMG-CoA reductase;
DE            EC=1.1.1.34;
GN   Name=HMGR;
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Little Delicata; TISSUE=Seedling;
RX   PubMed=16653173;
RA   Maldenado-Mendoza I.E., Burnett R.J., Nessler C.L.;
RT   "Nucleotide sequence of a cDNA encoding 3-hydroxy-3-methylglutaryl-CoA
RT   reductase from Catharanthus roseus.";
RL   Plant Physiol. 100:1613-1614(1992).
CC   -!- FUNCTION: Catalyzes the synthesis of mevalonate. The specific
CC       precursor of all isoprenoid compounds present in plants.
CC   -!- CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-
CC       hydroxy-3-methylglutaryl-CoA + 2 NADPH.
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid
CC       biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the HMG-CoA reductase family.
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DR   EMBL; M96068; AAA33108.1; -; mRNA.
DR   PIR; T09967; T09967.
DR   HSSP; P04035; 1HWI.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR   GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH)...; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002202; HMG_CoA_Rdtase_cat.
DR   InterPro; IPR004554; HMG_CoA_Rdtase_I_cat.
DR   Gene3D; G3DSA:3.90.770.10; HMG-CoA_red; 1.
DR   PANTHER; PTHR10572; HMG-CoA_red; 1.
DR   Pfam; PF00368; HMG-CoA_red; 1.
DR   PRINTS; PR00071; HMGCOARDTASE.
DR   TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR   PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR   PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR   PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR   PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane;
KW   NADP; Oxidoreductase; Transmembrane.
FT   CHAIN         1    601       3-hydroxy-3-methylglutaryl-coenzyme A
FT                                reductase.
FT                                /FTId=PRO_0000114437.
FT   TRANSMEM     36     58       Potential.
FT   TRANSMEM     86    106       Potential.
FT   REGION      107    179       Linker (By similarity).
FT   REGION      180    601       Catalytic (By similarity).
FT   ACT_SITE    273    273       Charge relay system (By similarity).
FT   ACT_SITE    405    405       Charge relay system (By similarity).
FT   ACT_SITE    481    481       Charge relay system (By similarity).
FT   ACT_SITE    579    579       Proton donor (By similarity).
FT   CARBOHYD    337    337       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    583    583       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   601 AA;  64107 MW;  D49D00D4F5DB9113 CRC64;
     MDSRRRSPTV TAKAAAGELP LAPHEGQNQQ PSIPRSSDVL PLPLYLANGV FFTLFFSVMY
     FLLTRWREKI RNATPLHVVT LSELAALASL IASVIYLVSF FGLDFVQSLI YKPNNEGWEI
     EEEILMVEDS RNGTNCTTLG CAVPPPSVPK IAPVVPQQPS KMVIIEKPAP LITPQNSEED
     EDIIKAVVAG KIPSYSLESK LGDCKRAAGI RREALQRITG KSLEGLPLEG FDYASILGQC
     CEMPVGYVQL PVGIAGPLLL DGREYMLPMA TTEGCLVAST NRGCKAILAS GGANSVLLRD
     GMTRAPVVRF GTAKRAAELK FYMEDTQNFE TISVVFNKSS RFAKLQSVQC AIAGKNLYIR
     FSCSTGDAMG MNMVSKGVQN VLEFLQTDYP DMDVLGISGN FCADKKPAAV NWIEGRGKSV
     VCEAIIKEEI VKTVLKTEVA ALIELNMVKN LAGSAIAGAL GGFNAHASNI VSAIFIATGQ
     DPAQNVESSQ CITMMEAVND GKDLHISVTM PSIEVGTVGG GTQLASQSAC LNLLGVKGAS
     KDSPGANSRL LATIVAGSVL AGELSLMSAI SAGQLVRSHM KYNRSSKDIT NIASSQLESD
     S
//