NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 6258 / CBS 573 / DSM 6128 / JCM 1609 / NBRC 1064;
PubMed=7989540 [NCBI, ExPASy, EBI, Israel, Japan]
Burgener-Kairuz P., Zuber J.P., Jaunin P., Buchman T.G., Bille J., Rossier M.;
"Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment.";
J. Clin. Microbiol. 32:1902-1907(1994).

Comments

FUNCTION: Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.
CATALYTIC ACTIVITY: Obtusifoliol + 3 O₂ + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24²⁸-trien-3-beta-ol + formate + 3 NADP⁺ + 4 H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Steroid metabolism; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6 .
SUBCELLULAR LOCATION: Membrane (Potential). Membrane; Single-pass membrane protein.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S75391; AAB32680.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q02315.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00465; EP450IV.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; PARTIAL.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Heme; Iron; Lipid synthesis; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 >414`	`>414`	`Cytochrome P450 51.`	`PRO_0000052007`
`NON_TER`	`414 414`

Sequence information

Length: 414 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 46981 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: A23CCD0EE1F3A72D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPWVGSAVVY GMQPYEFFEN CRKQHGDVFS FLLLGKVMTV YLGPKGHEFV LNAKLSDVSA 

        70         80         90        100        110        120 
EDAYTHLTTP VFGKGVIYDC PNWKLMEQKK FAKVALTKES FIRYVPLIKD EMLKYFNANF 

       130        140        150        160        170        180 
RGDSGKTDVL KSQSEMTLFT ASRSLFGDAL RNRLDASYAE MYSDLDKGFT PLNFVFSYLP 

       190        200        210        220        230        240 
LPNYWKRDAA HKNISNTYLD LINTKRAGGE IKNEDLVDAL LKNSVYKDGT RMTDEELAHL 

       250        260        270        280        290        300 
MIGVLMGGQH TSSATSAWFL LHLGEKPQLQ EEIYREIQSV LGENFERELT YDDLQKLDLV 

       310        320        330        340        350        360 
NATIKETLRL HMPLHSIFRK VTRDLPVPNT SYIVPKGHYV LISPGYTMLS ERYFPNASEF 

       370        380        390        400        410 
QPHRWDEIKS IDGGISFGAE GENAKETVDY GFGKISKGVA SPYLPFGGGR HRCT

Q02315 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools