NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-22.
STRAIN=PKO1;
PubMed=1400204 [NCBI, ExPASy, EBI, Israel, Japan]
Kukor J.J., Olsen R.H.;
"Complete nucleotide sequence of tbuD, the gene encoding phenol/cresol hydroxylase from Pseudomonas pickettii PKO1, and functional analysis of the encoded enzyme.";
J. Bacteriol. 174:6518-6526(1992).

Comments

FUNCTION: Hydroxylates phenol to catechol. Also acts on cresols.
CATALYTIC ACTIVITY: Phenol + NADPH + O₂ = catechol + NADP⁺ + H₂O.
COFACTOR: FAD.
PATHWAY: Aromatic compound metabolism; phenol degradation .
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the pheA/tfdB FAD monooxygenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M98806; AAA25992.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A45730; A45730.

3D structure databases

ModBase

Q01551.

Ontologies

GO:0018662;	Molecular function: phenol 2-monooxygenase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR002938; mOase_FAD_bd.
IPR003042; Rng_hydrolase.
Graphical view of domain structure.

Pfam

PF01494; FAD_binding_3; 2.
Pfam graphical view of domain structure.

PRINTS

PR00368; FADPNR.
PR00420; RNGMNOXGNASE.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Aromatic hydrocarbons catabolism; Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 671`	`670`	`Phenol 2-monooxygenase.`	`PRO_0000214047`
`NP_BIND`	`10 43`	`34`	`FAD (Potential).`
`NP_BIND`	`295 305`	`11`	`FAD (Potential).`

Sequence information

Length: 671 AA [This is the length of the unprocessed precursor]

Molecular weight: 72999 Da [This is the MW of the unprocessed precursor]

CRC64: A7ADFB9286640195 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTKYNEAYCD VLIVGAGPAG VMAAAHLLSY GTTARPHRVR IFDATKEVNG SDESTESLST 

        70         80         90        100        110        120 
DVIADALNSG ASGPEKDAAS TTEDLPMLVT TLQVSDVLHD TGDDTKIAYR ETATEQQVLL 

       130        140        150        160        170        180 
LADTTANTSS TMNPRSMCEA GCRFHQIYQG HCFPEYELDS ERLRSVDGRA QVLEDEHETG 

       190        200        210        220        230        240 
QLRLERLGRP EELLELDEEN SMSVVTNLKA APYKFLMKDV DENFPGELST SGGKTTSISA 

       250        260        270        280        290        300 
DESAIDAALH AVWDADDLGA AWHLDEASGL RAVDWNAAQW FKSGQPWTPD AAKSLQEGRV 

       310        320        330        340        350        360 
FLAGDARHRH PPLTGIGKNT SIADCYNLTW KLLGVLLGVA RADPARTYVA ERVYIRMRAA 

       370        380        390        400        410        420 
TDIAVDAEME SLAAKWITVQ LTLSRSWISS AKEAERWDAV LRDSAMSASK PMWTTSDMRA 

       430        440        450        460        470        480 
SFDAGLMGHG HAHDHVTPTI KEFASSSISR SISELASTSW WESRGWGNGG PFESLMEDAR 

       490        500        510        520        530        540 
WTGAVESNCR YAAYDRDAPV LHEHVAWVTR FTSRARTAVL EAAVGQAHVV DCWDVGLVEP 

       550        560        570        580        590        600 
ALDDLDSAGA GLHVAHHADQ WPAQLDEAVW PRESLSDWRI VTDTSATGEG YQTSPREAPG 

       610        620        630        640        650        660 
DYADLNADNA KAHFNGQFAG HKAYGDAAAA DGGGCHGRIL VGPAVRGRHL HREIPLGEEC 

       670 
QRAAQPLFKE V

Q01551 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools