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UniProtKB/Swiss-Prot entry Q01462


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LDH1_LACLA
Primary accession number Q01462
Secondary accession numbers None
Integrated into Swiss-Prot on April 1, 1993
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 77)
Name and origin of the protein
Protein name L-lactate dehydrogenase 1
Synonyms L-LDH 1
EC 1.1.1.27
Gene name
Name: ldhA
Synonyms: ldh
OrderedLocusNames: LL1331
ORFNames: L0017
From
Lactococcus lactis subsp. lactis (Streptococcus lactis) [TaxID: 1360] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=LM0230;
PubMed=1400245 [NCBI, ExPASy, EBI, Israel, Japan]
Llanos R.M., Hillier A.J., Davidson B.E.;
"Cloning, nucleotide sequence, expression, and chromosomal location of ldh, the gene encoding L-(+)-lactate dehydrogenase, from Lactococcus lactis.";
J. Bacteriol. 174:6956-6964(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(92)90049-U; PubMed=1452029 [NCBI, ExPASy, EBI, Israel, Japan]
Griffin H.G., Swindell S.R., Gasson M.G.;
"Cloning and sequence analysis of the gene encoding L-lactate dehydrogenase from Lactococcus lactis: evolutionary relationships between 21 different LDH enzymes.";
Gene 122:193-197(1992).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=BU2-60, and IL1403;
PubMed=8081494 [NCBI, ExPASy, EBI, Israel, Japan]
Swindell S.R., Griffin H.G., Gasson M.J.;
"Cloning, sequencing and comparison of three lactococcal L-lactate dehydrogenase genes.";
Microbiology 140:1301-1305(1994).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=1117M, 112, BEN121, and BO34;
PubMed=9023947 [NCBI, ExPASy, EBI, Israel, Japan]
Urbach E., Daniels B., Salama M.S., Sandine W.E., Giovannoni S.J.;
"The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes.";
Appl. Environ. Microbiol. 63:694-702(1997).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=IL1403;
DOI=10.1101/gr.GR-1697R; PubMed=11337471 [NCBI, ExPASy, EBI, Israel, Japan]
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.;
"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403.";
Genome Res. 11:731-753(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M95919; AAA25172.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M88490; AAA25187.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L07920; AAA99896.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U04315; AAA61963.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U02386; AAA61962.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U78632; AAB51676.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U78636; AAB51680.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U78637; AAB51681.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U78638; AAB51682.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U78634; AAB51678.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE006365; AAK05429.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A45246; A45246.
C86791; C86791.
JN0449; JN0449.
RefSeq NP_267487.1; -.
3D structure databases
HSSP P00343; 1LLC. [HSSP ENTRY / PDB]
ModBase Q01462.
Enzyme and pathway databases
BioCyc LLAC272623:L0017-MON; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0004459; Molecular function: L-lactate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0019642; Biological process: anaerobic glycolysis (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00488; -; 1.
PBIL [Tree]
InterPro IPR001557; L-lactate/malate_DHase.
IPR011304; L-lactate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
TIGRFAMs TIGR01771; L-LDH-NAD; 1.
PROSITE PS00064; L_LDH; 1.
BLOCKS Q01462.
Genome annotation databases
GeneID 1114981; -.
GenomeReviews AE005176_GR; LL1331.
KEGG lla:L0017; -.
NMPDR fig|272623.1.peg.1366; -.
Phylogenomic databases
HOGENOM Q01462; -.
Genome annotation databases
CMR Q01462; LL1331.
Other
ProtoNet Q01462.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   325  324     L-lactate dehydrogenase 1. PRO_0000168351
NP_BIND   15    43  29     NAD (By similarity). 
ACT_SITE   178   178        Proton acceptor (By similarity). 
BINDING   91    91        Substrate (By similarity). 
BINDING   123   123        NAD or substrate (By similarity). 
BINDING   154   154        Substrate (By similarity). 
BINDING   232   232        Substrate (By similarity). 
MOD_RES   223   223        Phosphotyrosine (By similarity). 
CONFLICT   270   270        C -> F (in Ref. 1; AAA25172/AAA99896 and 4; AAB51678). 
Sequence information
Length: 325 AA [This is the length of the unprocessed precursor] Molecular weight: 35051 Da [This is the MW of the unprocessed precursor] CRC64: 8BFA78343AF13003 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MADKQRKKVI LVGDGAVGSS YAFALVNQGI AQELGIVDLF KEKTQGDAED LSHALAFTSP 

        70         80         90        100        110        120 
KKIYSADYSD ASDADLVVLT SGAPQKPGET RLDLVEKNLR ITKDVVTKIV ASGFKGIFLV 

       130        140        150        160        170        180 
AANPVDILTY ATWKFSGFPK NRVVGSGTSL DTARFRQALA EKVDVDARSI HAYIMGEHGD 

       190        200        210        220        230        240 
SEFAVWSHAN VAGVKLEQWF QENDYLNEAE IVELFESVRD AAYSIIAKKG ATFYGVAVAL 

       250        260        270        280        290        300 
ARITKAILDD EHAVLPVSVF QDGQYGVSDC YLGQPAVVGA EGVVNPIHIP LNDAEMQKME 

       310        320 
ASGAQLKAII DEAFAKEEFA SAVKN 

Q01462 in FASTA format

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