ID   CTF4_YEAST              Reviewed;         927 AA.
AC   Q01454;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-DEC-2008, entry version 81.
DE   RecName: Full=DNA polymerase alpha-binding protein;
DE   AltName: Full=Chromosome transmission fidelity protein 4;
DE   AltName: Full=Protein POB1;
DE   AltName: Full=Chromosome replication protein CHL15;
GN   Name=CTF4; Synonyms=CHL15, POB1; OrderedLocusNames=YPR135W;
GN   ORFNames=P9659.7;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-21.
RC   STRAIN=7208-12;
RX   MEDLINE=93078774; PubMed=1448101;
RA   Miles J., Formosa T.;
RT   "Evidence that POB1, a Saccharomyces cerevisiae protein that binds to
RT   DNA polymerase alpha, acts in DNA metabolism in vivo.";
RL   Mol. Cell. Biol. 12:5724-5735(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93078775; PubMed=1341195;
RA   Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V.,
RA   Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M.,
RA   Hieter P., Spencer F., Larionov V.;
RT   "CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast
RT   Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 12:5736-5747(1992).
RN   [3]
RP   ERRATUM.
RA   Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V.,
RA   Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M.,
RA   Hieter P., Spencer F., Larionov V.;
RL   Mol. Cell. Biol. 13:7202-7202(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93302761; PubMed=8316240;
RA   Kouprina N.Y., Kroll E.S., Koryabin M.Y., Shestopalov B.V.,
RA   Bliskovsky V.V., Bannikov V.M., Gizatullin R.Z., Kirillov A.V.,
RA   Kravtsov V.Y., Zakharyev V.M.;
RT   "CHL15 -- a new gene controlling the replication of chromosomes in
RT   Saccharomycetes yeast: cloning, physical mapping, sequencing, and
RT   sequence analysis.";
RL   Mol. Biol. (Mosk.) 27:569-588(1993).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=97313271; PubMed=9169875;
RA   Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA   Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V.,
RA   Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M.,
RA   Chung E., Churcher C.M., Coster F., Davis K., Davis R.W.,
RA   Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A.,
RA   Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A.,
RA   Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W.,
RA   Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K.,
RA   Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J.,
RA   Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D.,
RA   Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA   Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA   Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA   Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA   Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V.,
RA   Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W.,
RA   Zollner A., Vo D.H., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL   Nature 387:103-105(1997).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377 AND SER-379, AND
RP   MASS SPECTROMETRY.
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-379 AND
RP   SER-463, AND MASS SPECTROMETRY.
RX   PubMed=17563356; DOI=10.1073/pnas.0701622104;
RA   Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
RT   "Proteome-wide identification of in vivo targets of DNA damage
RT   checkpoint kinases.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-379; THR-401;
RP   THR-411 AND SER-463, AND MASS SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Accessory factor for DNA replication. It plays a role in
CC       accurately duplicating the genome in vivo.
CC   -!- INTERACTION:
CC       P33203:PRP40; NbExp=1; IntAct=EBI-5209, EBI-701;
CC       P39940:RSP5; NbExp=1; IntAct=EBI-5209, EBI-16219;
CC       P40318:SSM4; NbExp=1; IntAct=EBI-5209, EBI-18208;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Present with 3280 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Contains 5 WD repeats.
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DR   EMBL; M94769; AAA34887.1; -; Genomic_DNA.
DR   EMBL; S63246; AAB27308.1; -; Genomic_DNA.
DR   EMBL; U40829; AAB68276.1; -; Genomic_DNA.
DR   PIR; A45039; A45039.
DR   RefSeq; NP_015461.1; -.
DR   DIP; DIP:4640N; -.
DR   IntAct; Q01454; 10.
DR   PeptideAtlas; Q01454; -.
DR   Ensembl; YPR135W; Saccharomyces cerevisiae.
DR   GeneID; 856254; -.
DR   GenomeReviews; U00094_GR; YPR135W.
DR   KEGG; sce:YPR135W; -.
DR   NMPDR; fig|4932.3.peg.6598; -.
DR   CYGD; YPR135w; -.
DR   SGD; S000006339; CTF4.
DR   HOGENOM; Q01454; -.
DR   LinkHub; Q01454; -.
DR   NextBio; 981537; -.
DR   ArrayExpress; Q01454; -.
DR   GermOnline; YPR135W; Saccharomyces cerevisiae.
DR   GO; GO:0043596; C:nuclear replication fork; IDA:SGD.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0006281; P:DNA repair; IGI:SGD.
DR   GO; GO:0006261; P:DNA-dependent DNA replication; IMP:SGD.
DR   GO; GO:0007064; P:mitotic sister chromatid cohesion; IMP:SGD.
DR   GO; GO:0001302; P:replicative cell aging; IMP:SGD.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like.
DR   InterPro; IPR001680; WD40_repeat.
DR   Gene3D; G3DSA:2.130.10.10; WD40/YVTN_repeat-like; 1.
DR   SMART; SM00320; WD40; 3.
DR   PROSITE; PS00678; WD_REPEATS_1; FALSE_NEG.
DR   PROSITE; PS50082; WD_REPEATS_2; FALSE_NEG.
DR   PROSITE; PS50294; WD_REPEATS_REGION; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Direct protein sequencing; DNA replication;
KW   Nucleus; Phosphoprotein; Repeat; WD repeat.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    927       DNA polymerase alpha-binding protein.
FT                                /FTId=PRO_0000050948.
FT   REPEAT       10     49       WD 1.
FT   REPEAT      134    173       WD 2.
FT   REPEAT      227    266       WD 3.
FT   REPEAT      273    313       WD 4.
FT   REPEAT      699    739       WD 5.
FT   MOD_RES     377    377       Phosphoserine.
FT   MOD_RES     379    379       Phosphoserine.
FT   MOD_RES     401    401       Phosphothreonine.
FT   MOD_RES     411    411       Phosphothreonine.
FT   MOD_RES     463    463       Phosphoserine.
FT   CONFLICT    112    112       G -> E (in Ref. 2 and 4).
FT   CONFLICT    588    588       V -> F (in Ref. 2).
FT   CONFLICT    590    590       V -> F (in Ref. 2).
FT   CONFLICT    601    601       N -> I (in Ref. 2).
FT   CONFLICT    835    835       E -> K (in Ref. 2).
SQ   SEQUENCE   927 AA;  104425 MW;  E2ABBB244B5F8B4D CRC64;
     MVSVIDKLVF DFGGKTLVSL APDNNTLCVA NKNGLTKILK TNNPEEEPET LDSSKLVSSI
     KCYSNSHFLM TTMQGDALRY NIDSSQEELL ARFALPLRDC CVIHSGKMAV FGGDDLELIL
     LELDDETHKK HAIKIDEQVS QISYNSQMNI LAVSMINGKV QIFSLTSTIP NKVHELNDYI
     VANSYDDTHR DKILSNMMDD IDKDNDNDLS ETADPDENNV ADPEFCAANR ICTRVAWHPK
     GLHFALPCAD DTVKIFSIKG YSLQKTLSTN LSSTKAHFID LQFDPLRGTY IAAVDLNNKL
     TVWNWETSEI HYTREFKRKI TNIAWKIQAD SKTLDLVLGT WSGSIAIVQN LAESVVSNIP
     DQSVAESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI TQEANAEDVF TQTHDGPSGL
     SEKRKYNFED EEDFIDDDDG AGYISGKKPH NEHSYSRVHK THSFPISLAN TGKFRYMPFS
     PAGTPFGFTD RRYLTMNEVG YVSTVKNSEQ YSITVSFFDV GRFREYHFED LFGYDLCFLN
     EKGTLFGQSK TGQIQYRPHD SIHSNWTKII PLQAGERITS VAATPVRVIV GTSLGYFRSF
     NQFGVPFAVE KTSPIVALTA QNYRVFSVHY SQFHGLSYSL SELGTSSKRY YKRECPLPMS
     LPNINSDMKK DANLDYYNFN PMGIKSLFFS SYGDPCIFGS DNTLLLLSKW RSPEESKWLP
     ILDSNMEIWK MSGGKETTDI HVWPLALAYD TLNCILVKGK HIWPEFPLPL PSEMEIRMPV
     FVKSKLLEEN KAILNKKNEI GADTEAEEGE EDKEIQIPVS MAAEEEYLRS KVLSELLTDT
     LENDGEMYGN ENEVLAALNG AYDKALLRLF ASACSDQNVE KALSLAHELK QDRALTAAVK
     ISERAELPSL VKKINNIREA RYEQQLK
//