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UniProtKB/Swiss-Prot entry Q01454


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CTF4_YEAST
Primary accession number Q01454
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1993
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 80)
Name and origin of the protein
Protein name DNA polymerase alpha-binding protein
Synonyms Chromosome transmission fidelity protein 4
Protein POB1
Chromosome replication protein CHL15
Gene name
Name: CTF4
Synonyms: CHL15, POB1
OrderedLocusNames: YPR135W
ORFNames: P9659.7
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-21.
STRAIN=7208-12;
PubMed=1448101 [NCBI, ExPASy, EBI, Israel, Japan]
Miles J., Formosa T.;
"Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo.";
Mol. Cell. Biol. 12:5724-5735(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1341195 [NCBI, ExPASy, EBI, Israel, Japan]
Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V., Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M., Hieter P., Spencer F., Larionov V.;
"CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae.";
Mol. Cell. Biol. 12:5736-5747(1992).
[3]
ERRATUM.
Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V., Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M., Hieter P., Spencer F., Larionov V.;
Mol. Cell. Biol. 13:7202-7202(1993).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8316240 [NCBI, ExPASy, EBI, Israel, Japan]
Kouprina N.Y., Kroll E.S., Koryabin M.Y., Shestopalov B.V., Bliskovsky V.V., Bannikov V.M., Gizatullin R.Z., Kirillov A.V., Kravtsov V.Y., Zakharyev V.M.;
"CHL15 -- a new gene controlling the replication of chromosomes in Saccharomycetes yeast: cloning, physical mapping, sequencing, and sequence analysis.";
Mol. Biol. (Mosk.) 27:569-588(1993).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169875 [NCBI, ExPASy, EBI, Israel, Japan]
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
Nature 387:103-105(1997).
[6]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377 AND SER-379, AND MASS SPECTROMETRY.
DOI=10.1021/pr060559j; PubMed=17330950 [NCBI, ExPASy, EBI, Israel, Japan]
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.;
"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.";
J. Proteome Res. 6:1190-1197(2007).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-379 AND SER-463, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0701622104; PubMed=17563356 [NCBI, ExPASy, EBI, Israel, Japan]
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.";
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
[9]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-379; THR-401; THR-411 AND SER-463, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M94769; AAA34887.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S63246; AAB27308.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U40829; AAB68276.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A45039; A45039.
RefSeq NP_015461.1; -.
3D structure databases
ModBase Q01454.
Protein-protein interaction databases
DIP DIP:4640N; -.
IntAct Q01454; -.
Organism-specific databases
CYGD YPR135w; -.
SGD S000006339; CTF4.
Yeast-GFP YPR135W.
Gene expression databases
ArrayExpress Q01454; -.
GermOnline YPR135W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0043596; Cellular component: nuclear replication fork (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006281; Biological process: DNA repair (inferred from genetic interaction from SGD).
GO:0006261; Biological process: DNA-dependent DNA replication (inferred from mutant phenotype from SGD).
GO:0007064; Biological process: mitotic sister chromatid cohesion (inferred from mutant phenotype from SGD).
GO:0001302; Biological process: replicative cell aging (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR015943; WD40/YVTN_repeat-like.
IPR001680; WD40_repeat.
Graphical view of domain structure.
Gene3D G3DSA:2.130.10.10; WD40/YVTN_repeat-like; 1.
SMART SM00320; WD40; 3.
SMART graphical view of domain structure.
PROSITE PS00678; WD_REPEATS_1; FALSE_NEG.
PS50082; WD_REPEATS_2; FALSE_NEG.
PS50294; WD_REPEATS_REGION; FALSE_NEG.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q01454.
ProtoNet Q01454.
Proteomic databases
PeptideAtlas Q01454; -.
Genome annotation databases
Ensembl YPR135W; Saccharomyces cerevisiae. [Contig view]
GeneID 856254; -.
GenomeReviews U00094_GR; YPR135W.
KEGG sce:YPR135W; -.
NMPDR fig|4932.3.peg.6598; -.
Phylogenomic databases
HOGENOM Q01454; -.
Other
LinkHub Q01454; -.
NextBio 981537; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; DNA replication; Nucleus; Phosphoprotein; Repeat; WD repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   927  926     DNA polymerase alpha-binding protein. PRO_0000050948
REPEAT   10    49  40     WD 1. 
REPEAT   134   173  40     WD 2. 
REPEAT   227   266  40     WD 3. 
REPEAT   273   313  41     WD 4. 
REPEAT   699   739  41     WD 5. 
MOD_RES   377   377        Phosphoserine. 
MOD_RES   379   379        Phosphoserine. 
MOD_RES   401   401        Phosphothreonine. 
MOD_RES   411   411        Phosphothreonine. 
MOD_RES   463   463        Phosphoserine. 
CONFLICT   112   112        G -> E (in Ref. 2 and 4). 
CONFLICT   588   588        V -> F (in Ref. 2). 
CONFLICT   590   590        V -> F (in Ref. 2). 
CONFLICT   601   601        N -> I (in Ref. 2). 
CONFLICT   835   835        E -> K (in Ref. 2). 
Sequence information
Length: 927 AA [This is the length of the unprocessed precursor] Molecular weight: 104425 Da [This is the MW of the unprocessed precursor] CRC64: E2ABBB244B5F8B4D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVSVIDKLVF DFGGKTLVSL APDNNTLCVA NKNGLTKILK TNNPEEEPET LDSSKLVSSI 

        70         80         90        100        110        120 
KCYSNSHFLM TTMQGDALRY NIDSSQEELL ARFALPLRDC CVIHSGKMAV FGGDDLELIL 

       130        140        150        160        170        180 
LELDDETHKK HAIKIDEQVS QISYNSQMNI LAVSMINGKV QIFSLTSTIP NKVHELNDYI 

       190        200        210        220        230        240 
VANSYDDTHR DKILSNMMDD IDKDNDNDLS ETADPDENNV ADPEFCAANR ICTRVAWHPK 

       250        260        270        280        290        300 
GLHFALPCAD DTVKIFSIKG YSLQKTLSTN LSSTKAHFID LQFDPLRGTY IAAVDLNNKL 

       310        320        330        340        350        360 
TVWNWETSEI HYTREFKRKI TNIAWKIQAD SKTLDLVLGT WSGSIAIVQN LAESVVSNIP 

       370        380        390        400        410        420 
DQSVAESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI TQEANAEDVF TQTHDGPSGL 

       430        440        450        460        470        480 
SEKRKYNFED EEDFIDDDDG AGYISGKKPH NEHSYSRVHK THSFPISLAN TGKFRYMPFS 

       490        500        510        520        530        540 
PAGTPFGFTD RRYLTMNEVG YVSTVKNSEQ YSITVSFFDV GRFREYHFED LFGYDLCFLN 

       550        560        570        580        590        600 
EKGTLFGQSK TGQIQYRPHD SIHSNWTKII PLQAGERITS VAATPVRVIV GTSLGYFRSF 

       610        620        630        640        650        660 
NQFGVPFAVE KTSPIVALTA QNYRVFSVHY SQFHGLSYSL SELGTSSKRY YKRECPLPMS 

       670        680        690        700        710        720 
LPNINSDMKK DANLDYYNFN PMGIKSLFFS SYGDPCIFGS DNTLLLLSKW RSPEESKWLP 

       730        740        750        760        770        780 
ILDSNMEIWK MSGGKETTDI HVWPLALAYD TLNCILVKGK HIWPEFPLPL PSEMEIRMPV 

       790        800        810        820        830        840 
FVKSKLLEEN KAILNKKNEI GADTEAEEGE EDKEIQIPVS MAAEEEYLRS KVLSELLTDT 

       850        860        870        880        890        900 
LENDGEMYGN ENEVLAALNG AYDKALLRLF ASACSDQNVE KALSLAHELK QDRALTAAVK 

       910        920 
ISERAELPSL VKKINNIREA RYEQQLK 

Q01454 in FASTA format

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