[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=FVB/N; TISSUE=Mammary tumor; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[2]	NUCLEOTIDE SEQUENCE [MRNA] OF 664-887. PubMed=2258621 [NCBI, ExPASy, EBI, Israel, Japan] Helmberg A., Faessler R., Geley S., Joehrer K., Kroemer G., Boeck G., Kofler R.; "Glucocorticoid-regulated gene expression in the immune system. Analysis of glucocorticoid-regulated transcripts from the mouse macrophage-like cell line P388D1."; J. Immunol. 145:4332-4337(1990).

Comments

FUNCTION: This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP⁺ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3 .
PATHWAY: Context: Cholesterol biosynthesis .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Peroxisome membrane; Multi-pass membrane protein (By similarity).
SIMILARITY: Belongs to the HMG-CoA reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC085083; AAH85083.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M62766; AAA37819.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A43533; A43533.

UniGene

Mm.316652

3D structure databases

HSSP

P04035; 1DQA. [HSSP ENTRY / PDB]

Q01237; 461-869.

PTM databases

Q01237; -.

Organism-specific databases

MGI

MGI:96159; Hmgcr.

Gene expression databases

ArrayExpress

Q01237; -.

CleanEx

MM_HMGCR; -.

GermOnline

ENSMUSG00000021670; Mus musculus.

Ontologies

GO:0005792;	Cellular component: microsome (inferred from direct assay from MGI).
GO:0004420;	Molecular function: hydroxymethylglutaryl-CoA reductase (NADPH) activity (inferred from direct assay from MGI).
GO:0006695;	Biological process: cholesterol biosynthetic process (inferred from direct assay from MGI).
GO:0009790;	Biological process: embryonic development (inferred from mutant phenotype from MGI).
GO:0043407;	Biological process: negative regulation of MAP kinase activity (inferred from genetic interaction from MGI).
GO:0008542;	Biological process: visual learning (inferred from genetic interaction from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR002202; HMG_CoA_Rdtase_cat.
IPR004554; HMG_CoA_Rdtase_I_cat.
IPR004816; HMG_CoA_Rdtase_I_metazoan.
IPR000731; SSD_5TM.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.770.10; HMG-CoA_red; 1.

PANTHER

PTHR10572; HMG-CoA_red; 1.

Pfam

PF00368; HMG-CoA_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00071; HMGCOARDTASE.

TIGRFAMs

TIGR00920; 2A060605; 1.
TIGR00533; HMG_CoA_R_NADP; 1.

PROSITE

PS00066; HMG_COA_REDUCTASE_1; 1.
PS00318; HMG_COA_REDUCTASE_2; 1.
PS01192; HMG_COA_REDUCTASE_3; 1.
PS50065; HMG_COA_REDUCTASE_4; 1.
PS50156; SSD; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q01237.

Genome annotation databases

Ensembl

ENSMUSG00000021670; Mus musculus. [Contig view]

Phylogenomic databases

HOGENOM

Q01237; -.

HOVERGEN

Q01237; -.

Other

Hmgcr; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol biosynthesis; Endoplasmic reticulum; Glycoprotein; Lipid synthesis; Membrane; NADP; Oxidoreductase; Peroxisome; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 887`	`887`	`3-hydroxy-3-methylglutaryl-coenzyme A reductase.`	`PRO_0000114421`
`TRANSMEM`	`10 39`	`30`	`Potential.`
`TRANSMEM`	`57 78`	`22`	`Potential.`
`TRANSMEM`	`90 114`	`25`	`Potential.`
`TRANSMEM`	`124 149`	`26`	`Potential.`
`TRANSMEM`	`160 187`	`28`	`Potential.`
`TRANSMEM`	`192 220`	`29`	`Potential.`
`TRANSMEM`	`315 339`	`25`	`Potential.`
`REGION`	`340 449`	`110`	`Linker.`
`REGION`	`450 887`	`438`	`Catalytic.`
`ACT_SITE`	`558 558`		`Charge relay system (By similarity).`
`ACT_SITE`	`690 690`		`Charge relay system (By similarity).`
`ACT_SITE`	`766 766`		`Charge relay system (By similarity).`
`ACT_SITE`	`865 865`		`Proton donor (By similarity).`
`CARBOHYD`	`281 281`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`517 517`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`869 869`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 887 AA [This is the length of the unprocessed precursor]

Molecular weight: 97022 Da [This is the MW of the unprocessed precursor]

CRC64: 30F3B40D0BEE37A6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLSRLFRMHG LFVASHPWEV IVGTVTLTIC MMSMNMFTGN NKICGWNYEC PKFEEDVLSS 

        70         80         90        100        110        120 
DIIILTITRC IAILYIYFQF QNLRQLGSKY ILGIAGLFTI FSSFVFSTVV IHFLDKELTG 

       130        140        150        160        170        180 
LNEALPFFLL LIDLSRASAL AKFALSSNSQ DEVRENIARG MAILGPTFTL DALVECLVIG 

       190        200        210        220        230        240 
VGTMSGVRQL EIMCCFGCMS VLANYFVFMT FFPACVSLVL ELSRESREGR PIWQLSHFAR 

       250        260        270        280        290        300 
VLEEEENKPN PVTQRVKIIM SLGLVLVHAH SRWIADPSPQ NSTAEQAKVS LGLDEDVSKR 

       310        320        330        340        350        360 
IEPSVSLWQF YLSKMISMDI EQVITLSLAF LLAVKYIFFE QAETESTLSL KNPITSPVVT 

       370        380        390        400        410        420 
SKKAQDNCCR REPLLVRRNQ KLSSVEEDPG ANQERKVEVI KPLVVEAETT SRATFVLGAS 

       430        440        450        460        470        480 
VASPPSALGT QEPGIELPIE PRPNEECLQI LENAEKGAKF LSDAEIIQLV NAKHIPAYKL 

       490        500        510        520        530        540 
ETLMETHERG VSIRRQLLST KLAEPSSLQY LPYRDYNYSL VMGACCENVI GYMPIPVGVA 

       550        560        570        580        590        600 
GPLCLDGKEY QVPMATTEGC LVASTNRGCR AISLGGGASS RVLADGMTRG PVVRLPRACD 

       610        620        630        640        650        660 
SAEVKTWLET PEGFAVIKEA FDSTSRFARL QKLHVTMAGR NLYIRFQSRT GDAMGMNMIS 

       670        680        690        700        710        720 
KGTEKALLKL QEFFPDMQIL AVSGNYCTDK KPAAINWIEG RGKTVVCEAV IPAKVVREVL 

       730        740        750        760        770        780 
KTTTEAMVDV NINKNLVGSA MAGSIGGYNA HAANIVTAIY IACGQDAAQN VGSSNCITLM 

       790        800        810        820        830        840 
EASGPTNEDL YISCTMPSIE IGTVGGGTNL LPQQACLQML GVQGACKDNP GENARQLARI 

       850        860        870        880 
VCGTVMAGEL SLMAALAAGH LVRSHMVHNR SKINLQDLQG TCTKKAA

Q01237 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools