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UniProtKB/Swiss-Prot entry Q00960


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NMDE2_RAT
Primary accession number Q00960
Secondary accession number Q62684
Integrated into Swiss-Prot on June 1, 1994
Sequence was last modified on June 1, 1994 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 81)
Name and origin of the protein
Protein name Glutamate [NMDA] receptor subunit epsilon-2 [Precursor]
Synonyms N-methyl D-aspartate receptor subtype 2B
NMDAR2B
NR2B
Gene name
Name: Grin2b
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Brain;
PubMed=1350383 [NCBI, ExPASy, EBI, Israel, Japan]
Monyer H., Sprengel R., Schoepfer R., Herb A., Higuchi M., Lomeli H., Burnashev N., Sakmann B., Seeburg P.H.;
"Heteromeric NMDA receptors: molecular and functional distinction of subtypes.";
Science 256:1217-1221(1992).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/0896-6273(94)90258-5; PubMed=7524561 [NCBI, ExPASy, EBI, Israel, Japan]
Sullivan J.M., Traynelis S.F., Chen H.S., Escobar W., Heinemann S.F., Lipton S.A.;
"Identification of two cysteine residues that are required for redox modulation of the NMDA subtype of glutamate receptor.";
Neuron 13:929-936(1994).
[3]
INTERACTION WITH DLG4.
PubMed=7569905 [NCBI, ExPASy, EBI, Israel, Japan]
Kornau H.C., Schenker L.T., Kennedy M.B., Seeburg P.H.;
"Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.";
Science 269:1737-1740(1995).
[4]
INTERACTION WITH INADL.
DOI=10.1006/mcne.1998.0679; PubMed=9647694 [NCBI, ExPASy, EBI, Israel, Japan]
Kurschner C., Mermelstein P.G., Holden W.T., Surmeier D.J.;
"CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins.";
Mol. Cell. Neurosci. 11:161-172(1998).
[5]
IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN3A AND PPP2CB.
PubMed=11588171 [NCBI, ExPASy, EBI, Israel, Japan]
Chan S.F., Sucher N.J.;
"An NMDA receptor signaling complex with protein phosphatase 2A.";
J. Neurosci. 21:7985-7992(2001).
[6]
IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A.
PubMed=11160393 [NCBI, ExPASy, EBI, Israel, Japan]
Perez-Otano I., Schulteis C.T., Contractor A., Lipton S.A., Trimmer J.S., Sucher N.J., Heinemann S.F.;
"Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors.";
J. Neurosci. 21:1228-1237(2001).
[7]
IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A.
DOI=10.1124/mol.62.5.1119; PubMed=12391275 [NCBI, ExPASy, EBI, Israel, Japan]
Al-Hallaq R.A., Jarabek B.R., Fu Z., Vicini S., Wolfe B.B., Yasuda R.P.;
"Association of NR3A with the N-methyl-D-aspartate receptor NR1 and NR2 subunits.";
Mol. Pharmacol. 62:1119-1127(2002).
[8]
IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A.
DOI=10.1152/jn.00531.2001; PubMed=11929923 [NCBI, ExPASy, EBI, Israel, Japan]
Sasaki Y.F., Rothe T., Premkumar L.S., Das S., Cui J., Talantova M.V., Wong H.-K., Gong X., Chan S.F., Zhang D., Nakanishi N., Sucher N.J., Lipton S.A.;
"Characterization and comparison of the NR3A subunit of the NMDA receptor in recombinant systems and primary cortical neurons.";
J. Neurophysiol. 87:2052-2063(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M91562; AAA41714.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U11419; AAA50554.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B43274; B43274.
RefSeq NP_036706.1; -.
UniGene Rn.9711
3D structure databases
HSSP P19491; 1FTK. [HSSP ENTRY / PDB]
SMR Q00960; 404-540, 662-802.
ModBase Q00960.
Protein-protein interaction databases
IntAct Q00960; -.
PTM databases
PhosphoSite Q00960; -.
Organism-specific databases
RGD 2738; Grin2b.
Gene expression databases
ArrayExpress Q00960; -.
GermOnline ENSRNOG00000008766; Rattus norvegicus.
Ontologies
GO
GO:0017146; Cellular component: N-methyl-D-aspartate selective glutamate receptor complex (inferred from direct assay from UniProtKB).
GO:0043005; Cellular component: neuron projection (inferred from direct assay from UniProtKB).
GO:0042734; Cellular component: presynaptic membrane (inferred from direct assay from UniProtKB).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from UniProtKB).
GO:0007613; Biological process: memory (inferred from direct assay from UniProtKB).
GO:0014049; Biological process: positive regulation of glutamate secretion (inferred from direct assay from UniProtKB).
GO:0043408; Biological process: regulation of MAPKKK cascade (inferred from mutant phenotype from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR015683; Glutamate_receptor-rel.
IPR001320; Iontro_glu_rcpt.
IPR001508; NMDA_rcpt.
Graphical view of domain structure.
PANTHER PTHR18966; Glut_Rec_Related; 1.
Pfam PF00060; Lig_chan; 1.
Pfam graphical view of domain structure.
PRINTS PR00177; NMDARECEPTOR.
SMART SM00079; PBPe; 1.
SMART graphical view of domain structure.
BLOCKS Q00960.
Genome annotation databases
Ensembl ENSRNOG00000008766; Rattus norvegicus. [Contig view]
GeneID 24410; -.
KEGG rno:24410; -.
Phylogenomic databases
HOVERGEN Q00960; -.
Other
ProtoNet Q00960.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Cell junction; Cell membrane; Glycoprotein; Ion transport; Ionic channel; Magnesium; Membrane; Phosphoprotein; Postsynaptic cell membrane; Receptor; Signal; Synapse; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
SIGNAL   1     26  26     Potential. 
CHAIN   27   1482  1456     Glutamate [NMDA] receptor subunit epsilon-2. PRO_0000011579
TOPO_DOM   27    557  531     Extracellular (Potential). 
TRANSMEM   558    578  21     Potential. 
TOPO_DOM   579    634  56     Cytoplasmic (Potential). 
TRANSMEM   635    655  21     Potential. 
TOPO_DOM   656    817  162     Extracellular (Potential). 
TRANSMEM   818    838  21     Potential. 
TOPO_DOM   839   1482  644     Cytoplasmic (Potential). 
MOTIF   1480   1482  3     PDZ-binding. 
COMPBIAS   984    989  6     Poly-His. 
SITE   615    615  1     Functional determinant of NMDA receptors (By similarity). 
MOD_RES   917    917        Phosphoserine (By similarity). 
MOD_RES   930    930        Phosphoserine (By similarity). 
MOD_RES   932    932        Phosphotyrosine (By similarity). 
MOD_RES   949    949        Phosphotyrosine (By similarity). 
MOD_RES   962    962        Phosphotyrosine (By similarity). 
MOD_RES   1039   1039        Phosphotyrosine (By similarity). 
MOD_RES   1049   1049        Phosphotyrosine (By similarity). 
MOD_RES   1061   1061        Phosphoserine (By similarity). 
MOD_RES   1065   1065        Phosphothreonine (By similarity). 
MOD_RES   1070   1070        Phosphotyrosine (By similarity). 
MOD_RES   1109   1109        Phosphotyrosine (By similarity). 
MOD_RES   1155   1155        Phosphotyrosine (By similarity). 
MOD_RES   1166   1166        Phosphoserine (By similarity). 
MOD_RES   1252   1252        Phosphotyrosine (By similarity). 
MOD_RES   1303   1303        Phosphoserine (By similarity). 
MOD_RES   1304   1304        Phosphotyrosine (By similarity). 
MOD_RES   1472   1472        Phosphotyrosine (By similarity). 
CARBOHYD   74     74        N-linked (GlcNAc...) (Potential). 
CARBOHYD   341    341        N-linked (GlcNAc...) (Potential). 
CARBOHYD   348    348        N-linked (GlcNAc...) (Potential). 
CARBOHYD   444    444        N-linked (GlcNAc...) (Potential). 
CARBOHYD   491    491        N-linked (GlcNAc...) (Potential). 
CARBOHYD   542    542        N-linked (GlcNAc...) (Potential). 
CARBOHYD   688    688        N-linked (GlcNAc...) (Potential). 
CONFLICT   1256   1256        E -> K (in Ref. 2; AAA50554). 
CONFLICT   1430   1431        VT -> SA (in Ref. 2; AAA50554). 
Sequence information
Length: 1482 AA [This is the length of the unprocessed precursor] Molecular weight: 166071 Da [This is the MW of the unprocessed precursor] CRC64: AEF8B9DF3C1B0D5D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKPSAECCSP KFWLVLAVLA VSGSKARSQK SPPSIGIAVI LVGTSDEVAI KDAHEKDDFH 

        70         80         90        100        110        120 
HLSVVPRVEL VAMNETDPKS IITRICDLMS DRKIQGVVFA DDTDQEAIAQ ILDFISAQTL 

       130        140        150        160        170        180 
TPILGIHGGS SMIMADKDES SMFFQFGPSI EQQASVMLNI MEEYDWYIFS IVTTYFPGYQ 

       190        200        210        220        230        240 
DFVNKIRSTI ENSFVGWELE EVLLLDMSLD DGDSKIQNQL KKLQSPIILL YCTKEEATYI 

       250        260        270        280        290        300 
FEVANSVGLT GYGYTWIVPS LVAGDTDTVP SEFPTGLISV SYDEWDYGLP ARVRDGIAII 

       310        320        330        340        350        360 
TTAASDMLSE HSFIPEPKSS CYNTHEKRIY QSNMLNRYLI NVTFEGRNLS FSEDGYQMHP 

       370        380        390        400        410        420 
KLVIILLNKE RKWERVGKWK DKSLQMKYYV WPRMCPETEE QEDDHLSIVT LEEAPFVIVE 

       430        440        450        460        470        480 
SVDPLSGTCM RNTVPCQKRI ISENKTDEEP GYIKKCCKGF CIDILKKISK SVKFTYDLYL 

       490        500        510        520        530        540 
VTNGKHGKKI NGTWNGMIGE VVMKRAYMAV GSLTINEERS EVVDFSVPFI ETGISVMVSR 

       550        560        570        580        590        600 
SNGTVSPSAF LEPFSADVWV MMFVMLLIVS AVAVFVFEYF SPVGYNRCLA DGREPGGPSF 

       610        620        630        640        650        660 
TIGKAIWLLW GLVFNNSVPV QNPKGTTSKI MVSVWAFFAV IFLASYTANL AAFMIQEEYV 

       670        680        690        700        710        720 
DQVSGLSDKK FQRPNDFSPP FRFGTVPNGS TERNIRNNYA EMHAYMGKFN QRGVDDALLS 

       730        740        750        760        770        780 
LKTGKLDAFI YDAAVLNYMA GRDEGCKLVT IGSGKVFAST GYGIAIQKDS GWKRQVDLAI 

       790        800        810        820        830        840 
LQLFGDGEME ELEALWLTGI CHNEKNEVMS SQLDIDNMAG VFYMLGAAMA LSLITFICEH 

       850        860        870        880        890        900 
LFYWQFRHCF MGVCSGKPGM VFSISRGIYS CIHGVAIEER QSVMNSPTAT MNNTHSNILR 

       910        920        930        940        950        960 
LLRTAKNMAN LSGVNGSPQS ALDFIRRESS VYDISEHRRS FTHSDCKSYN NPPCEENLFS 

       970        980        990       1000       1010       1020 
DYISEVERTF GNLQLKDSNV YQDHYHHHHR PHSIGSTSSI DGLYDCDNPP FTTQPRSISK 

      1030       1040       1050       1060       1070       1080 
KPLDIGLPSS KHSQLSDLYG KFSFKSDRYS GHDDLIRSDV SDISTHTVTY GNIEGNAAKR 

      1090       1100       1110       1120       1130       1140 
RKQQYKDSLK KRPASAKSRR EFDEIELAYR RRPPRSPDHK RYFRDKEGLR DFYLDQFRTK 

      1150       1160       1170       1180       1190       1200 
ENSPHWEHVD LTDIYKERSD DFKRDSVSGG GPCTNRSHLK HGTGEKHGVV GGVPAPWEKN 

      1210       1220       1230       1240       1250       1260 
LTNVDWEDRS GGNFCRSCPS KLHNYSSTVA GQNSGRQACI RCEACKKAGN LYDISEDNSL 

      1270       1280       1290       1300       1310       1320 
QELDQPAAPV AVTSNASSTK YPQSPTNSKA QKKNRNKLRR QHSYDTFVDL QKEEAALAPR 

      1330       1340       1350       1360       1370       1380 
SVSLKDKGRF MDGSPYAHMF EMPAGESSFA NKSSVPTAGH HHNNPGSGYM LSKSLYPDRV 

      1390       1400       1410       1420       1430       1440 
TQNPFIPTFG DDQCLLHGSK SYFFRQPTVA GASKTRPDFR ALVTNKPVVV TLHGAVPGRF 

      1450       1460       1470       1480 
QKDICIGNQS NPCVPNNKNP RAFNGSSNGH VYEKLSSIES DV 

Q00960 in FASTA format

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