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UniProtKB/Swiss-Prot entry P49597


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Entry information
Entry name PP2C1_ARATH
Primary accession number P49597
Secondary accession numbers Q0WW30 Q43717 Q94C87
Integrated into Swiss-Prot on February 1, 1996
Sequence was last modified on June 6, 2002 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 86)
Name and origin of the protein
Protein name Protein phosphatase 2C ABI1
Synonyms PP2C ABI1
EC 3.1.3.16
Protein ABSCISIC ACID-INSENSITIVE 1
Gene name
Name: ABI1
OrderedLocusNames: At4g26080
ORFNames: F20B18.190
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
TISSUE=Leaf;
DOI=10.1016/0092-8674(94)90282-8; PubMed=7923358 [NCBI, ExPASy, EBI, Israel, Japan]
Mindrinos M., Katagiri F., Yu G.-L., Ausubel F.M.;
"The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats.";
Cell 78:1089-1099(1994).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
TISSUE=Leaf;
PubMed=7910981 [NCBI, ExPASy, EBI, Israel, Japan]
Leung J., Bouvier-Durand M., Morris P.C., Guerrier D., Chefdor F., Giraudat J.;
"Arabidopsis ABA response gene ABI1: features of a calcium-modulated protein phosphatase.";
Science 264:1448-1452(1994).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF GLY-180.
STRAIN=cv. Landsberg erecta;
PubMed=8197457 [NCBI, ExPASy, EBI, Israel, Japan]
Meyer K., Leube M.P., Grill E.;
"A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana.";
Science 264:1452-1455(1994).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/47134; PubMed=10617198 [NCBI, ExPASy, EBI, Israel, Japan]
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.;
"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
Nature 402:769-777(1999).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K., Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.;
"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
[7]
FUNCTION.
DOI=10.1111/j.1399-3054.1984.tb06343.x
Kornneef M., Reuling G., Karssen C.M.;
"The isolation and characterization of abscisic-acid insensitive mutants of Arabidopsis thaliana.";
Physiol. Plantarum 61:377-383(1984).
[8]
FUNCTION.
DOI=10.1007/BF00028738; PubMed=1834244 [NCBI, ExPASy, EBI, Israel, Japan]
Gilmour S.J., Thomashow M.F.;
"Cold acclimation and cold-regulated gene expression in ABA mutants of Arabidopsis thaliana.";
Plant Mol. Biol. 17:1233-1240(1991).
[9]
FUNCTION.
PubMed=16652949 [NCBI, ExPASy, EBI, Israel, Japan]
Schnall J.A., Quatrano R.S.;
"Abscisic acid elicits the water-stress response in root hairs of Arabidopsis thaliana.";
Plant Physiol. 100:216-218(1992).
[10]
FUNCTION.
DOI=10.1007/BF00291999; PubMed=8492808 [NCBI, ExPASy, EBI, Israel, Japan]
Finkelstein R.R.;
"Abscisic acid-insensitive mutations provide evidence for stage-specific signal pathways regulating expression of an Arabidopsis late embryogenesis-abundant (lea) gene.";
Mol. Gen. Genet. 238:401-408(1993).
[11]
FUNCTION.
PubMed=12232276 [NCBI, ExPASy, EBI, Israel, Japan]
Finkelstein R.R.;
"Maternal effects govern variable dominance of two abscisic acid response mutations in Arabidopsis thaliana.";
Plant Physiol. 105:1203-1208(1994).
[12]
FUNCTION.
PubMed=12232124 [NCBI, ExPASy, EBI, Israel, Japan]
Vartanian N., Marcotte L., Giraudat J.;
"Drought rhizogenesis in Arabidopsis thaliana (differential responses of hormonal mutants).";
Plant Physiol. 104:761-767(1994).
[13]
FUNCTION.
PubMed=7568166 [NCBI, ExPASy, EBI, Israel, Japan]
Armstrong F., Leung J., Grabov A., Brearley J., Giraudat J., Blatt M.R.;
"Sensitivity to abscisic acid of guard-cell K+ channels is suppressed by abi1-1, a mutant Arabidopsis gene encoding a putative protein phosphatase.";
Proc. Natl. Acad. Sci. U.S.A. 92:9520-9524(1995).
[14]
FUNCTION.
PubMed=12228349 [NCBI, ExPASy, EBI, Israel, Japan]
Maentylae E., Laang V., Palva E.T.;
"Role of abscisic acid in drought-induced freezing tolerance, cold acclimation, and accumulation of lt178 and rab18 proteins in Arabidopsis thaliana.";
Plant Physiol. 107:141-148(1995).
[15]
FUNCTION, MUTAGENESIS OF GLY-180, CATALYTIC ACTIVITY, AND COFACTORS.
DOI=10.1111/j.1432-1033.1996.0193t.x; PubMed=8898906 [NCBI, ExPASy, EBI, Israel, Japan]
Bertauche N., Leung J., Giraudat J.;
"Protein phosphatase activity of abscisic acid insensitive 1 (ABI1) protein from Arabidopsis thaliana.";
Eur. J. Biochem. 241:193-200(1996).
[16]
FUNCTION.
DOI=10.1046/j.1365-313X.1996.10020375.x; PubMed=8771791 [NCBI, ExPASy, EBI, Israel, Japan]
Soederman E., Mattsson J., Engstroem P.;
"The Arabidopsis homeobox gene ATHB-7 is induced by water deficit and by abscisic acid.";
Plant J. 10:375-381(1996).
[17]
FUNCTION.
DOI=10.1007/s004380050397; PubMed=9108297 [NCBI, ExPASy, EBI, Israel, Japan]
Savoure A., Hua X.-J., Bertauche N., Van Montagu M., Verbruggen N.;
"Abscisic acid-independent and abscisic acid-dependent regulation of proline biosynthesis following cold and osmotic stresses in Arabidopsis thaliana.";
Mol. Gen. Genet. 254:104-109(1997).
[18]
FUNCTION.
DOI=10.1105/tpc.9.3.409; PubMed=9090884 [NCBI, ExPASy, EBI, Israel, Japan]
Pei Z.-M., Kuchitsu K., Ward J.M., Schwarz M., Schroeder J.I.;
"Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants.";
Plant Cell 9:409-423(1997).
[19]
FUNCTION.
DOI=10.1105/tpc.9.5.759; PubMed=9165752 [NCBI, ExPASy, EBI, Israel, Japan]
Leung J., Merlot S., Giraudat J.;
"The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.";
Plant Cell 9:759-771(1997).
[20]
FUNCTION.
DOI=10.1046/j.1365-313X.1997.11040693.x; PubMed=9161030 [NCBI, ExPASy, EBI, Israel, Japan]
Parcy F., Giraudat J.;
"Interactions between the ABI1 and the ectopically expressed ABI3 genes in controlling abscisic acid responses in Arabidopsis vegetative tissues.";
Plant J. 11:693-702(1997).
[21]
FUNCTION.
DOI=10.1046/j.1365-313X.1997.12010203.x; PubMed=9263461 [NCBI, ExPASy, EBI, Israel, Japan]
Grabov A., Leung J., Giraudat J., Blatt M.R.;
"Alteration of anion channel kinetics in wild-type and abi1-1 transgenic Nicotiana benthamiana guard cells by abscisic acid.";
Plant J. 12:203-213(1997).
[22]
FUNCTION.
DOI=10.1046/j.1365-313X.1997.00557.x; PubMed=9351242 [NCBI, ExPASy, EBI, Israel, Japan]
Strizhov N., Abraham E., Oekresz L., Blickling S., Zilberstein A., Schell J., Koncz C., Szabados L.;
"Differential expression of two P5CS genes controlling proline accumulation during salt-stress requires ABA and is regulated by ABA1, ABI1 and AXR2 in Arabidopsis.";
Plant J. 12:557-569(1997).
[23]
FUNCTION.
DOI=10.1104/pp.114.4.1557; PubMed=9276963 [NCBI, ExPASy, EBI, Israel, Japan]
Webb A.A.R., Hetherington A.M.;
"Convergence of the abscisic acid, CO2, and extracellular calcium signal transduction pathways in stomatal guard cells.";
Plant Physiol. 114:1557-1560(1997).
[24]
CATALYTIC ACTIVITY, MUTAGENESIS OF GLY-180, BIOPHYSICOCHEMICAL PROPERTIES, AND COFACTOR.
DOI=10.1016/S0014-5793(98)00149-5; PubMed=9537523 [NCBI, ExPASy, EBI, Israel, Japan]
Leube M.P., Grill E., Amrhein N.;
"ABI1 of Arabidopsis is a protein serine/threonine phosphatase highly regulated by the proton and magnesium ion concentration.";
FEBS Lett. 424:100-104(1998).
[25]
FUNCTION, AND MUTAGENESIS OF ASP-93; 141-MET--ASP-143; GLY-174; 177-ASP--HIS-179 AND GLY-180.
DOI=10.1073/pnas.95.3.975; PubMed=9448270 [NCBI, ExPASy, EBI, Israel, Japan]
Sheen J.;
"Mutational analysis of protein phosphatase 2C involved in abscisic acid signal transduction in higher plants.";
Proc. Natl. Acad. Sci. U.S.A. 95:975-980(1998).
[26]
FUNCTION, AND CATALYTIC ACTIVITY.
PubMed=10645425 [NCBI, ExPASy, EBI, Israel, Japan]
Leung J., Merlot S., Gosti F., Bertauche N., Blatt M.R., Giraudat J.;
"The role of ABI1 in abscisic acid signal transduction: from gene to cell.";
Symp. Soc. Exp. Biol. 51:65-71(1998).
[27]
FUNCTION.
DOI=10.1105/tpc.11.9.1785; PubMed=10488243 [NCBI, ExPASy, EBI, Israel, Japan]
Allen G.J., Kuchitsu K., Chu S.P., Murata Y., Schroeder J.I.;
"Arabidopsis abi1-1 and abi2-1 phosphatase mutations reduce abscisic acid-induced cytoplasmic calcium rises in guard cells.";
Plant Cell 11:1785-1798(1999).
[28]
FUNCTION, AND MUTAGENESIS OF GLY-180; ALA-185; CYS-259; ARG-304; GLY-307; SER-314; PRO-328 AND SER-416.
DOI=10.1105/tpc.11.10.1897; PubMed=10521520 [NCBI, ExPASy, EBI, Israel, Japan]
Gosti F., Beaudoin N., Serizet C., Webb A.A.R., Vartanian N., Giraudat J.;
"ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling.";
Plant Cell 11:1897-1909(1999).
[29]
FUNCTION.
PubMed=10950871 [NCBI, ExPASy, EBI, Israel, Japan]
Arenas-Huertero F., Arroyo A., Zhou L., Sheen J., Leon P.;
"Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar.";
Genes Dev. 14:2085-2096(2000).
[30]
FUNCTION.
DOI=10.1007/s004250050692; PubMed=10872217 [NCBI, ExPASy, EBI, Israel, Japan]
Chak R.K.F., Thomas T.L., Quatrano R.S., Rock C.D.;
"The genes ABI1 and ABI2 are involved in abscisic acid- and drought-inducible expression of the Daucus carota L. Dc3 promoter in guard cells of transgenic Arabidopsis thaliana (L.) Heynh.";
Planta 210:875-883(2000).
[31]
FUNCTION.
DOI=10.1073/pnas.231471998; PubMed=11707572 [NCBI, ExPASy, EBI, Israel, Japan]
Morillon R., Chrispeels M.J.;
"The role of ABA and the transpiration stream in the regulation of the osmotic water permeability of leaf cells.";
Proc. Natl. Acad. Sci. U.S.A. 98:14138-14143(2001).
[32]
FUNCTION.
DOI=10.1046/j.1365-313x.2001.00965.x; PubMed=11208021 [NCBI, ExPASy, EBI, Israel, Japan]
Merlot S., Gosti F., Guerrier D., Vavasseur A., Giraudat J.;
"The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway.";
Plant J. 25:295-303(2001).
[33]
FUNCTION.
DOI=10.1023/A:1011667312754; PubMed=11587514 [NCBI, ExPASy, EBI, Israel, Japan]
Shen Q., Gomez-Cadenas A., Zhang P., Walker-Simmons M.K., Sheen J., Ho T.-H.D.;
"Dissection of abscisic acid signal transduction pathways in barley aleurone layers.";
Plant Mol. Biol. 47:437-448(2001).
[34]
FUNCTION.
DOI=10.1007/s004250000489; PubMed=11289613 [NCBI, ExPASy, EBI, Israel, Japan]
Eun S.-O., Bae S.-H., Lee Y.;
"Cortical actin filaments in guard cells respond differently to abscisic acid in wild-type and abi1-1 mutant Arabidopsis.";
Planta 212:466-469(2001).
[35]
INDUCTION.
DOI=10.1046/j.1365-313X.2001.2641048.x; PubMed=11439132 [NCBI, ExPASy, EBI, Israel, Japan]
Taehtiharju S., Palva T.;
"Antisense inhibition of protein phosphatase 2C accelerates cold acclimation in Arabidopsis thaliana.";
Plant J. 26:461-470(2001).
[36]
FUNCTION.
DOI=10.1105/tpc.13.11.2513; PubMed=11701885 [NCBI, ExPASy, EBI, Israel, Japan]
Murata Y., Pei Z.-M., Mori I.C., Schroeder J.;
"Abscisic acid activation of plasma membrane Ca(2+) channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1-1 and abi2-1 protein phosphatase 2C mutants.";
Plant Cell 13:2513-2523(2001).
[37]
FUNCTION, AND INTERACTION WITH CIPK15/PKS3.
DOI=10.1016/S1534-5807(02)00229-0; PubMed=12194854 [NCBI, ExPASy, EBI, Israel, Japan]
Guo Y., Xiong L., Song C.-P., Gong D., Halfter U., Zhu J.-K.;
"A calcium sensor and its interacting protein kinase are global regulators of abscisic acid signaling in Arabidopsis.";
Dev. Cell 3:233-244(2002).
[38]
FUNCTION, MUTAGENESIS OF ASP-177 AND GLY-180, AND INTERACTION WITH ATHB-6.
DOI=10.1093/emboj/cdf316; PubMed=12065416 [NCBI, ExPASy, EBI, Israel, Japan]
Himmelbach A., Hoffmann T., Leube M., Hoehener B., Grill E.;
"Homeodomain protein ATHB6 is a target of the protein phosphatase ABI1 and regulates hormone responses in Arabidopsis.";
EMBO J. 21:3029-3038(2002).
[39]
FUNCTION.
DOI=10.1242/jcs.00175; PubMed=12432076 [NCBI, ExPASy, EBI, Israel, Japan]
Hoth S., Morgante M., Sanchez J.-P., Hanafey M.K., Tingey S.V., Chua N.-H.;
"Genome-wide gene expression profiling in Arabidopsis thaliana reveals new targets of abscisic acid and largely impaired gene regulation in the abi1-1 mutant.";
J. Cell Sci. 115:4891-4900(2002).
[40]
FUNCTION.
DOI=10.1046/j.1365-313X.2002.01322.x; PubMed=12047634 [NCBI, ExPASy, EBI, Israel, Japan]
Merlot S., Mustilli A.-C., Genty B., North H., Lefebvre V., Sotta B., Vavasseur A., Giraudat J.;
"Use of infrared thermal imaging to isolate Arabidopsis mutants defective in stomatal regulation.";
Plant J. 30:601-609(2002).
[41]
FUNCTION.
DOI=10.1046/j.1365-313X.2003.01721.x; PubMed=12713537 [NCBI, ExPASy, EBI, Israel, Japan]
Wu Y., Sanchez J.P., Lopez-Molina L., Himmelbach A., Grill E., Chua N.-H.;
"The abi1-1 mutation blocks ABA signaling downstream of cADPR action.";
Plant J. 34:307-315(2003).
[42]
FUNCTION.
DOI=10.1104/pp.103.026294; PubMed=14576281 [NCBI, ExPASy, EBI, Israel, Japan]
Stepansky A., Galili G.;
"Synthesis of the Arabidopsis bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase enzyme of lysine catabolism is concertedly regulated by metabolic and stress-associated signals.";
Plant Physiol. 133:1407-1415(2003).
[43]
FUNCTION.
DOI=10.1016/S0014-5793(03)01118-9; PubMed=14596925 [NCBI, ExPASy, EBI, Israel, Japan]
Becker D., Hoth S., Ache P., Wenkel S., Roelfsema M.R.G., Meyerhoff O., Hartung W., Hedrich R.;
"Regulation of the ABA-sensitive Arabidopsis potassium channel gene GORK in response to water stress.";
FEBS Lett. 554:119-126(2003).
[44]
FUNCTION.
DOI=10.1046/j.1365-313X.2003.01656.x; PubMed=12609042 [NCBI, ExPASy, EBI, Israel, Japan]
Fryer M.J., Ball L., Oxborough K., Karpinski S., Mullineaux P.M., Baker N.R.;
"Control of Ascorbate Peroxidase 2 expression by hydrogen peroxide and leaf water status during excess light stress reveals a functional organisation of Arabidopsis leaves.";
Plant J. 33:691-705(2003).
[45]
INDUCTION BY ABA.
DOI=10.1046/j.1365-313X.2003.01966.x; PubMed=14731256 [NCBI, ExPASy, EBI, Israel, Japan]
Saez A., Apostolova N., Gonzalez-Guzman M., Gonzalez-Garcia M.P., Nicolas C., Lorenzo O., Rodriguez P.L.;
"Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling.";
Plant J. 37:354-369(2004).
[46]
FUNCTION.
DOI=10.1111/j.1365-313X.2004.02086.x; PubMed=15144382 [NCBI, ExPASy, EBI, Israel, Japan]
Chini A., Grant J.J., Seki M., Shinozaki K., Loake G.J.;
"Drought tolerance established by enhanced expression of the CC-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1.";
Plant J. 38:810-822(2004).
[47]
FUNCTION, MUTAGENESIS OF 67-ARG-LYS-68 AND ARG-73, SUBCELLULAR LOCATION, ENZYME REGULATION, AND INTERACTION WITH PA.
DOI=10.1073/pnas.0402112101; PubMed=15197253 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang W., Qin C., Zhao J., Wang X.;
"Phospholipase D alpha 1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling.";
Proc. Natl. Acad. Sci. U.S.A. 101:9508-9513(2004).
[48]
GENE FAMILY, AND NOMENCLATURE.
DOI=10.1016/j.tplants.2004.03.007; PubMed=15130549 [NCBI, ExPASy, EBI, Israel, Japan]
Schweighofer A., Hirt H., Meskiene I.;
"Plant PP2C phosphatases: emerging functions in stress signaling.";
Trends Plant Sci. 9:236-243(2004).
[49]
FUNCTION.
DOI=10.1104/pp.104.053082; PubMed=15618419 [NCBI, ExPASy, EBI, Israel, Japan]
Christmann A., Hoffmann T., Teplova I., Grill E., Mueller A.;
"Generation of active pools of abscisic acid revealed by in vivo imaging of water-stressed Arabidopsis.";
Plant Physiol. 137:209-219(2005).
[50]
FUNCTION.
DOI=10.1104/pp.105.062257; PubMed=15923322 [NCBI, ExPASy, EBI, Israel, Japan]
Larkindale J., Hall J.D., Knight M.R., Vierling E.;
"Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance.";
Plant Physiol. 138:882-897(2005).
[51]
FUNCTION, AND INTERACTION WITH SRK2E.
DOI=10.1074/jbc.M509820200; PubMed=16365038 [NCBI, ExPASy, EBI, Israel, Japan]
Yoshida R., Umezawa T., Mizoguchi T., Takahashi S., Takahashi F., Shinozaki K.;
"The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis.";
J. Biol. Chem. 281:5310-5318(2006).
[52]
FUNCTION.
DOI=10.1093/jxb/erj026; PubMed=16339784 [NCBI, ExPASy, EBI, Israel, Japan]
Verslues P.E., Bray E.A.;
"Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potential-induced ABA and proline accumulation.";
J. Exp. Bot. 57:201-212(2006).
[53]
INTERACTION WITH GPX3, AND REPRESSION BY OXIDIZED GPX3.
DOI=10.1105/tpc.106.044230; PubMed=16998070 [NCBI, ExPASy, EBI, Israel, Japan]
Miao Y., Lv D., Wang P., Wang X.-C., Chen J., Miao C., Song C.-P.;
"An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses.";
Plant Cell 18:2749-2766(2006).
[54]
FUNCTION, AND MUTAGENESIS OF ASP-177.
DOI=10.1073/pnas.0501720103; PubMed=16571665 [NCBI, ExPASy, EBI, Israel, Japan]
Yang Y., Sulpice R., Himmelbach A., Meinhard M., Christmann A., Grill E.;
"Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection.";
Proc. Natl. Acad. Sci. U.S.A. 103:6061-6066(2006).
[55]
INDUCTION BY ABA, AND TISSUE SPECIFICITY.
DOI=10.1104/pp.105.070128; PubMed=16339800 [NCBI, ExPASy, EBI, Israel, Japan]
Yoshida T., Nishimura N., Kitahata N., Kuromori T., Ito T., Asami T., Shinozaki K., Hirayama T.;
"ABA-hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs.";
Plant Physiol. 140:115-126(2006).
[56]
FUNCTION.
DOI=10.1104/pp.106.081018; PubMed=16798945 [NCBI, ExPASy, EBI, Israel, Japan]
Saez A., Robert N., Maktabi M.H., Schroeder J.I., Serrano R., Rodriguez P.L.;
"Enhancement of abscisic acid sensitivity and reduction of water consumption in Arabidopsis by combined inactivation of the protein phosphatases type 2C ABI1 and HAB1.";
Plant Physiol. 141:1389-1399(2006).
[57]
FUNCTION, INTERACTION WITH PA, ENZYME REGULATION, AND MUTAGENESIS OF ARG-73.
DOI=10.1126/science.1123769; PubMed=16614222 [NCBI, ExPASy, EBI, Israel, Japan]
Mishra G., Zhang W., Deng F., Zhao J., Wang X.;
"A bifurcating pathway directs abscisic acid effects on stomatal closure and opening in Arabidopsis.";
Science 312:264-266(2006).
[58]
INTERACTION WITH SCAR1; SCAR2; SCAR3 AND SCARL.
DOI=10.1242/dev.02792; PubMed=17267444 [NCBI, ExPASy, EBI, Israel, Japan]
Uhrig J.F., Mutondo M., Zimmermann I., Deeks M.J., Machesky L.M., Thomas P., Uhrig S., Rambke C., Hussey P.J., Huelskamp M.;
"The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis.";
Development 134:967-977(2007).
[59]
FUNCTION.
DOI=10.1038/sj.emboj.7601575; PubMed=17304219 [NCBI, ExPASy, EBI, Israel, Japan]
de Torres-Zabala M., Truman W., Bennett M.H., Lafforgue G., Mansfield J.W., Rodriguez Egea P., Bogre L., Grant M.;
"Pseudomonas syringae pv. tomato hijacks the Arabidopsis abscisic acid signalling pathway to cause disease.";
EMBO J. 26:1434-1443(2007).
[60]
FUNCTION, AND INDUCTION BY ETHYLENE.
DOI=10.1104/pp.106.092700; PubMed=17158582 [NCBI, ExPASy, EBI, Israel, Japan]
Benschop J.J., Millenaar F.F., Smeets M.E., van Zanten M., Voesenek L.A.C.J., Peeters A.J.M.;
"Abscisic acid antagonizes ethylene-induced hyponastic growth in Arabidopsis.";
Plant Physiol. 143:1013-1023(2007).
[61]
FUNCTION, MUTAGENESIS OF ASP-177; GLY-180 AND 425-ARG--LYS-427, NLS, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
DOI=10.1111/j.1365-313X.2008.03454.x; PubMed=18298671 [NCBI, ExPASy, EBI, Israel, Japan]
Moes D., Himmelbach A., Korte A., Haberer G., Grill E.;
"Nuclear localization of the mutant protein phosphatase abi1 is required for insensitivity towards ABA responses in Arabidopsis.";
Plant J. 54:806-819(2008).
[62]
INDUCTION BY MYB44 AND SALT.
DOI=10.1104/pp.107.110981; PubMed=18162593 [NCBI, ExPASy, EBI, Israel, Japan]
Jung C., Seo J.S., Han S.W., Koo Y.J., Kim C.H., Song S.I., Nahm B.H., Choi Y.D., Cheong J.-J.;
"Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis.";
Plant Physiol. 146:623-635(2008).
Comments
  • FUNCTION: Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as the actin reorganization in guard cells in response to ABA. Involved in the resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. Controls negatively fibrillin expression that is involved in mediating ABA-induced photoprotection. May be involved in ABA content regulation. Plays a role in the Pro accumulation in response to reduced water availability (low water potential). Required for the ABA negative regulation of the ethylene-induced hyponastic growth. Involved in acquired thermotolerance of root growth and seedling survival. Activates SRK2E/OST1 in response to ABA-dependent stimuli.
  • CATALYTIC ACTIVITY: A phosphoprotein + H2O = a protein + phosphate.
  • COFACTOR: Binds 2 magnesium or manganese ions per subunit.
  • ENZYME REGULATION: Phosphatase activity repressed by oxidized GPX3 and phosphatidic acid (PA). PA is produced by PLD alpha 1 in response to ABA.
  • BIOPHYSICOCHEMICAL PROPERTIES:
    pH dependence:   Optimum pH is 8;
  • SUBUNIT: Interacts with ATHB-6, CIPK15/PKS3, GPX3, SRK2E/OST1, SCAR1, SCAR2, SCAR3 and SCARL. Binds to the PA released by the phospholipase D alpha 1 (PLDALPHA1) in response to ABA during the stomatal closure regulation.
  • INTERACTION:
    Q940H6:SRK2E; NbExp=2; IntAct=EBI-782526, EBI-782514;
  • SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Associated to the plasma membrane when in complex with PA, subsequently to ABA signaling.
  • TISSUE SPECIFICITY: Expressed in seeds and seedlings. In roots, confined to lateral root caps and columella cells.
  • INDUCTION: Repressed by MYB44. Induced by low temperature, drought, high salt, ABA and ethylene.
  • MISCELLANEOUS: Enhanced ABA signaling repressor activity by the proteosomal inhibitor MG132 accompanied by a cytoplasmic localization.
  • MISCELLANEOUS: Plants insensitive to ABA (abi1-1) are more resitant to P.syringae.
  • SIMILARITY: Belongs to the PP2C family.
  • SIMILARITY: Contains 1 PP2C-like domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U12856; AAA50237.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X77116; CAA54383.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X78886; CAA55484.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL049483; CAB39673.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL161564; CAB79463.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY035073; AAK59578.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY142623; AAN13081.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK226529; BAE98668.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T04263; T04263.
RefSeq NP_194338.1; -.
UniGene At.21332
3D structure databases
HSSP P35813; 1A6Q. [HSSP ENTRY / PDB]
ModBase P49597.
Protein-protein interaction databases
IntAct P49597; -.
Organism-specific databases
TAIR At4g26080; -.
Gene expression databases
ArrayExpress P49597; -.
GermOnline AT4G26080; Arabidopsis thaliana.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR015655; PP2C.
IPR001932; PP2C-related.
IPR000222; PP2C_Mn2_Asp60_BS.
IPR014045; PP2C_N.
Graphical view of domain structure.
Gene3D G3DSA:3.60.40.10; PP2C-related; 1.
PANTHER PTHR13832; PP2C; 1.
Pfam PF00481; PP2C; 1.
Pfam graphical view of domain structure.
SMART SM00331; PP2C_SIG; 1.
SM00332; PP2Cc; 1.
SMART graphical view of domain structure.
PROSITE PS01032; PP2C; 1.
BLOCKS P49597.
ProtoNet P49597.
Genome annotation databases
GeneID 828714; -.
GenomeReviews CT486007_GR; AT4G26080.
KEGG ath:AT4G26080; -.