NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=9063468 [NCBI, ExPASy, EBI, Israel, Japan]
Kuenkel A., Vaupel M., Heim S., Thauer R.K., Hedderich R.;
"Heterodisulfide reductase from methanol-grown cells of Methanosarcina barkeri is not a flavoenzyme.";
Eur. J. Biochem. 244:226-234(1997).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
DOI=10.1073/pnas.032671499; PubMed=11930014 [NCBI, ExPASy, EBI, Israel, Japan]
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.;
"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.";
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).

Comments

FUNCTION: Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). May act as the catalytic subunit (By similarity).
CATALYTIC ACTIVITY: Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O³-phospho-L-threonine + dihydromethanophenazine.
COFACTOR: Binds 4 4Fe-4S clusters per subunit (By similarity).
COFACTOR: FAD (By similarity).
PATHWAY: Cofactor metabolism; coenzyme B/coenzyme M regeneration; coenzyme B and coenzyme M from CoB-CoM heterodisulfide: step 1/1 .
SUBUNIT: The heterodisulfide reductase is composed of three subunits; hdrA, hdrB and hdrC (By similarity).
SIMILARITY: Belongs to the hdrA family.
SIMILARITY: Contains 4 4Fe-4S ferredoxin-type domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Y09871; CAA70999.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AE010324; AAM01482.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_613552.1; -.

3D structure databases

HSSP

P00195; 1CLF. [HSSP ENTRY / PDB]

ModBase

P96801.

Enzyme and pathway databases

BioCyc

MKAN190192:MK0265-MON; -.

Family and domain databases

InterPro

IPR001450; 4Fe4S_Fe_S_bd.
IPR006076; FAD-dep_OxRdtase.
IPR016040; NAD(P)-bd.
IPR001100; Pyr_nuc-diS_OxRdtase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF01266; DAO; 1.
PF00037; Fer4; 4.
Pfam graphical view of domain structure.

PRINTS

PR00353; 4FE4SFRDOXIN.
PR00411; PNDRDTASEI.

PROSITE

PS00198; 4FE4S_FER_1; 3.
PS51379; 4FE4S_FER_2; 4.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P96801.

Genome annotation databases

1477568; -.

AE009439_GR; MK0265.

mka:MK0265; -.

fig|190192.1.peg.265; -.

Phylogenomic databases

HOGENOM

P96801; -.

Genome annotation databases

CMR

P96801; MK0265.

Other

ProtoNet

P96801.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; FAD; Flavoprotein; Iron; Iron-sulfur; Metal-binding; Methanogenesis; Oxidoreductase; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 656`	`656`	`CoB--CoM heterodisulfide reductase iron-sulfur subunit A 2.`	`PRO_0000150059`
`DOMAIN`	`238 269`	`32`	`4Fe-4S ferredoxin-type 1.`
`DOMAIN`	`286 315`	`30`	`4Fe-4S ferredoxin-type 2.`
`DOMAIN`	`577 606`	`30`	`4Fe-4S ferredoxin-type 3.`
`DOMAIN`	`610 639`	`30`	`4Fe-4S ferredoxin-type 4.`
`NP_BIND`	`152 175`	`24`	`FAD (Potential).`
`METAL`	`248 248`		`Iron-sulfur 1 (4Fe-4S) (Potential).`
`METAL`	`251 251`		`Iron-sulfur 1 (4Fe-4S) (Potential).`
`METAL`	`254 254`		`Iron-sulfur 1 (4Fe-4S) (Potential).`
`METAL`	`258 258`		`Iron-sulfur 2 (4Fe-4S) (Potential).`
`METAL`	`295 295`		`Iron-sulfur 2 (4Fe-4S) (Potential).`
`METAL`	`298 298`		`Iron-sulfur 2 (4Fe-4S) (Potential).`
`METAL`	`301 301`		`Iron-sulfur 2 (4Fe-4S) (Potential).`
`METAL`	`305 305`		`Iron-sulfur 1 (4Fe-4S) (Potential).`
`METAL`	`586 586`		`Iron-sulfur 3 (4Fe-4S) (Potential).`
`METAL`	`589 589`		`Iron-sulfur 3 (4Fe-4S) (Potential).`
`METAL`	`592 592`		`Iron-sulfur 3 (4Fe-4S) (Potential).`
`METAL`	`596 596`		`Iron-sulfur 4 (4Fe-4S) (Potential).`
`METAL`	`619 619`		`Iron-sulfur 4 (4Fe-4S) (Potential).`
`METAL`	`622 622`		`Iron-sulfur 4 (4Fe-4S) (Potential).`
`METAL`	`625 625`		`Iron-sulfur 4 (4Fe-4S) (Potential).`
`METAL`	`629 629`		`Iron-sulfur 3 (4Fe-4S) (Potential).`

Sequence information

Length: 656 AA [This is the length of the unprocessed precursor]

Molecular weight: 71925 Da [This is the MW of the unprocessed precursor]

CRC64: C7CF218545ACEA7C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAEEEPRIGV YVCHCGVNIA GVVDVKEVAE FAKTLKNVVV ARDYKYVCSD PGQEIIQRDI 

        70         80         90        100        110        120 
EKYDLNRVVV AACSPRLHEP TFRRCVEEAG LNPYCFEMAN IREHCSWVHM DDPARATEKA 

       130        140        150        160        170        180 
KDLVRMAVAK ARLLESLETI KVDVTDRALV IGGGVSGIQA ALDLADMGFE VILVEKEPSI 

       190        200        210        220        230        240 
GGRMAQLDKT FPTNDCSICI LAPKMVDVSK HPNIKMYTYA EVVEVDGYVG NFTVKIEKKP 

       250        260        270        280        290        300 
RYVDEDACTG CGACAEVCPI EVPNEFDEGL GMRKAIYKPF PQAVPSVFTI DEEHCIRCGL 

       310        320        330        340        350        360 
CEEVCDADAI DFDQEPEIVE EEVGAIICAI GYDTCDPTER EEYGYGVYDN VITSIELERL 

       370        380        390        400        410        420 
INASGPTGGK VVRPSDGKKP KRIAFIQCVG SRDPHRTNPY CSNVCCMYAM KLAQLIREKY 

       430        440        450        460        470        480 
PETQIDIYYM DVRAFGKGYE EYYERSQKQY GIRFIRGRPA EIVEDPETKN LIVRAEDTLL 

       490        500        510        520        530        540 
GDVVEREYDL VVLSVGMVPR DSADVIQEVL SISRSPDGFF MEAHPKLRPV DTAIDGIFLA 

       550        560        570        580        590        600 
GACQGPKDIP SSVAQGSAAA ARAATALAAG EVAVEPIVSE VDEEICGGCG TCVELCPYGA 

       610        620        630        640        650 
IELVEKDGKL VAEVTAALCK GCGTCAAACP SGAMEQNHFK TEQLYKQIEG AFRDPA

P96801 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools