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UniProtKB/Swiss-Prot entry P96417


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GABD2_MYCTU
Primary accession number P96417
Secondary accession number Q7D824
Integrated into Swiss-Prot on November 13, 2007
Sequence was last modified on July 5, 2004 (Sequence version 3)
Annotations were last modified on    September 2, 2008 (Entry version 55)
Name and origin of the protein
Protein name Putative succinate-semialdehyde dehydrogenase [NADP+] 2
Synonyms SSADH 2
SSDH 2
EC 1.2.1.16
Gene name
Name: gabD2
OrderedLocusNames: Rv1731, MT1772
From
Mycobacterium tuberculosis [TaxID: 1773] [HAMAP proteome]
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Corynebacterineae; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 25618 / H37Rv;
DOI=10.1038/31159; PubMed=9634230 [NCBI, ExPASy, EBI, Israel, Japan]
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.;
"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.";
Nature 393:537-544(1998).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=CDC 1551 / Oshkosh;
DOI=10.1128/JB.184.19.5479-5490.2002; PubMed=12218036 [NCBI, ExPASy, EBI, Israel, Japan]
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains.";
J. Bacteriol. 184:5479-5490(2002).
[3]
FUNCTION, AND CATALYTIC ACTIVITY.
STRAIN=ATCC 25618 / H37Rv;
DOI=10.1073/pnas.0501605102; PubMed=16027371 [NCBI, ExPASy, EBI, Israel, Japan]
Tian J., Bryk R., Itoh M., Suematsu M., Nathan C.;
"Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: identification of alpha-ketoglutarate decarboxylase.";
Proc. Natl. Acad. Sci. U.S.A. 102:10670-10675(2005).
Comments
  • FUNCTION: Catalyzes the NADP(+)-dependent oxidation of succinate semialdehyde to succinate. Although it has succinate semialdehyde dehydrogenase activity, is likely to act physiologically on a different aldehyde(s). NAD(+) can substitute for NADP(+), but enzymatic activity is three times reduced.
  • CATALYTIC ACTIVITY: Succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H.
  • SIMILARITY: Belongs to the aldehyde dehydrogenase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
BX842577; CAB07016.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000516; AAK46045.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR H70962; H70962.
RefSeq NP_214748.2; -.
NP_336231.1; -.
3D structure databases
HSSP P20000; 1AG8. [HSSP ENTRY / PDB]
ModBase P96417.
Organism-specific databases
TubercuList Rv1731; -.
Family and domain databases
InterPro IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
PANTHER PTHR11699; Aldehyde_dehyd; 1.
Pfam PF00171; Aldedh; 1.
Pfam graphical view of domain structure.
PROSITE PS00070; ALDEHYDE_DEHYDR_CYS; FALSE_NEG.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.
BLOCKS P96417.
Genome annotation databases
GeneID 885204; -.
923923; -.
GenomeReviews AE000516_GR; MT1772.
AL123456_GR; Rv1731.
KEGG mtc:MT1772; -.
mtu:Rv1731; -.
TIGR MT1772; -.
Phylogenomic databases
HOGENOM P96417; -.
Other
ProtoNet P96417.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   518  518     Putative succinate-semialdehyde dehydrogenase [NADP+] 2. PRO_0000310711
NP_BIND   232   237  6     NAD or NADP (By similarity). 
ACT_SITE   254   254        By similarity. 
ACT_SITE   288   288        By similarity. 
Sequence information
Length: 518 AA [This is the length of the unprocessed precursor] Molecular weight: 55324 Da [This is the MW of the unprocessed precursor] CRC64: E3AC9E895EB46270 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPAPSAEVFD RLRNLAAIKD VAARPTRTID EVFTGKPLTT IPVGTAADVE AAFAEARAAQ 

        70         80         90        100        110        120 
TDWAKRPVIE RAAVIRRYRD LVIENREFLM DLLQAEAGKA RWAAQEEIVD LIANANYYAR 

       130        140        150        160        170        180 
VCVDLLKPRK AQPLLPGIGK TTVCYQPKGV VGVISPWNYP MTLTVSDSVP ALVAGNAVVL 

       190        200        210        220        230        240 
KPDSQTPYCA LACAELLYRA GLPRALYAIV PGPGSVVGTA ITDNCDYLMF TGSSATGSRL 

       250        260        270        280        290        300 
AEHAGRRLIG FSAELGGKNP MIVARGANLD KVAKAATRAC FSNAGQLCIS IERIYVEKDI 

       310        320        330        340        350        360 
AEEFTRKFGD AVRNMKLGTA YDFSVDMGSL ISEAQLKTVS GHVDDATAKG AKVIAGGKAR 

       370        380        390        400        410        420 
PDIGPLFYEP TVLTNVAPEM ECAANETFGP VVSIYPVADV DEAVEKANDT DYGLNASVWA 

       430        440        450        460        470        480 
GSTAEGQRIA ARLRSGTVNV DEGYAFAWGS LSAPMGGMGL SGVGRRHGPE GLLKYTESQT 

       490        500        510 
IATARVFNLD PPFGIPATVW QKSLLPIVRT VMKLPGRR 

P96417 in FASTA format

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