[1]	NUCLEOTIDE SEQUENCE. STRAIN=NCIB 8401; PubMed=9294422 [NCBI, ExPASy, EBI, Israel, Japan] Pieulle L., Magro V., Hatchikian E.C.; "Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability."; J. Bacteriol. 179:5684-5692(1997).
[2]	NUCLEOTIDE SEQUENCE. STRAIN=NCIB 8401; Claude H.; Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
[3]	X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 2-1232. DOI=10.1038/5870; PubMed=10048931 [NCBI, ExPASy, EBI, Israel, Japan] Chabriere E., Charon M.H., Volbeda A., Pieulle L., Hatchikian E.C., Fontecilla-Camps J.C.; "Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate."; Nat. Struct. Biol. 6:182-190(1999).
[4]	X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 2-1232. DOI=10.1126/science.1066198; PubMed=11752578 [NCBI, ExPASy, EBI, Israel, Japan] Chabriere E., Vernede X., Guigliarelli B., Charon M.H., Hatchikian E.C., Fontecilla-Camps J.C.; "Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase."; Science 294:2559-2563(2001).

Comments

Copyright

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Cross-references

Sequence databases

EMBL

Y09702; CAA70873.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

PDB

1B0P; X-ray; 2.31 A; A/B=2-1232.	[ExPASy / RCSB / EBI]
1KEK; X-ray; 1.90 A; A/B=2-1232.	[ExPASy / RCSB / EBI]
2C3M; X-ray; 1.84 A; A/B=2-1232.	[ExPASy / RCSB / EBI]
2C3O; X-ray; 2.70 A; A/B=2-1232.	[ExPASy / RCSB / EBI]
2C3P; X-ray; 2.33 A; A/B=2-1232.	[ExPASy / RCSB / EBI]
2C3U; X-ray; 2.32 A; A/B=2-1232.	[ExPASy / RCSB / EBI]
2C3Y; X-ray; 1.93 A; A/B=2-1232.	[ExPASy / RCSB / EBI]
2C42; X-ray; 1.78 A; A/B=2-1232.	[ExPASy / RCSB / EBI]
2PDA; X-ray; 3.00 A; A/B=2-1232.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1B0P; -.
1KEK; -.
2C3M; -.
2C3O; -.
2C3P; -.
2C3U; -.
2C3Y; -.
2C42; -.
2PDA; -.

ModBase

P94692.

Protein-protein interaction databases

DIP

DIP:29036N; -.

Ontologies

GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0016903;	Molecular function: oxidoreductase activity, acting on the aldehyde or oxo group of donors (inferred from electronic annotation from InterPro).
GO:0030976;	Molecular function: thiamin pyrophosphate binding (inferred from electronic annotation from InterPro).
GO:0008152;	Biological process: metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR017896; 4Fe4S_Fe-S-bd.
IPR001450; 4Fe4S_Fe_S_bd_subgr.
IPR017900; 4Fe4S_Fe_S_CS.
IPR011895; Pyruv_OxRdtase_PFOR.
IPR002869; Pyrv_Fd/Flavodoxin_OxRdtase.
IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR011766; TPP_enzyme_bd_C.
IPR015941; Transketolase_C-like.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.920.10; Pyrv_Fd/Flavodoxin_OxRdtase; 1.
G3DSA:3.40.50.920; Transketo_C_like; 1.

Pfam

PF00037; Fer4; 2.
PF01558; POR; 1.
PF01855; POR_N; 1.
PF02775; TPP_enzyme_C; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000159; NifJ; 1.

TIGRFAMs

TIGR02176; pyruv_ox_red; 1.

PROSITE

PS00198; 4FE4S_FER_1; 2.
PS51379; 4FE4S_FER_2; 2.
PROSITE graphical view of domain structure (profiles).

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Iron; Iron-sulfur; Metal-binding; Pyruvate.

Features

None

Sequence information

Length: 1232 AA

Molecular weight: 133680 Da

CRC64: 55D84F536CCFFDBF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGKKMMTTDG NTATAHVAYA MSEVAAIYPI TPSSTMGEEA DDWAAQGRKN IFGQTLTIRE 

        70         80         90        100        110        120 
MQSEAGAAGA VHGALAAGAL TTTFTASQGL LLMIPNMYKI SGELLPGVFH VTARAIAAHA 

       130        140        150        160        170        180 
LSIFGDHQDI YAARQTGFAM LASSSVQEAH DMALVAHLAA IESNVPFMHF FDGFRTSHEI 

       190        200        210        220        230        240 
QKIEVLDYAD MASLVNQKAL AEFRAKSMNP EHPHVRGTAQ NPDIYFQGRE AANPYYLKVP 

       250        260        270        280        290        300 
GIVAEYMQKV ASLTGRSYKL FDYVGAPDAE RVIVSMGSSC ETIEEVINHL AAKGEKIGLI 

       310        320        330        340        350        360 
KVRLYRPFVS EAFFAALPAS AKVITVLDRT KEPGAPGDPL YLDVCSAFVE RGEAMPKILA 

       370        380        390        400        410        420 
GRYGLGSKEF SPAMVKSVYD NMSGAKKNHF TVGIEDDVTG TSLPVDNAFA DTTPKGTIQC 

       430        440        450        460        470        480 
QFWGLGADGT VGANKQAIKI IGDNTDLFAQ GYFSYDSKKS GGITISHLRF GEKPIQSTYL 

       490        500        510        520        530        540 
VNRADYVACH NPAYVGIYDI LEGIKDGGTF VLNSPWSSLE DMDKHLPSGI KRTIANKKLK 

       550        560        570        580        590        600 
FYNIDAVKIA TDVGLGGRIN MIMQTAFFKL AGVLPFEKAV DLLKKSIHKA YGKKGEKIVK 

       610        620        630        640        650        660 
MNTDAVDQAV TSLQEFKYPD SWKDAPAETK AEPMTNEFFK NVVKPILTQQ GDKLPVSAFE 

       670        680        690        700        710        720 
ADGRFPLGTS QFEKRGVAIN VPQWVPENCI QCNQCAFVCP HSAILPVLAK EEELVGAPAN 

       730        740        750        760        770        780 
FTALEAKGKE LKGYKFRIQI NTLDCMGCGN CADICPPKEK ALVMQPLDTQ RDAQVPNLEY 

       790        800        810        820        830        840 
AARIPVKSEV LPRDSLKGSQ FQEPLMEFSG ACSGCGETPY VRVITQLFGE RMFIANATGC 

       850        860        870        880        890        900 
SSIWGASAPS MPYKTNRLGQ GPAWGNSLFE DAAEYGFGMN MSMFARRTHL ADLAAKALES 

       910        920        930        940        950        960 
DASGDVKEAL QGWLAGKNDP IKSKEYGDKL KKLLAGQKDG LLGQIAAMSD LYTKKSVWIF 

       970        980        990       1000       1010       1020 
GGDGWAYDIG YGGLDHVLAS GEDVNVFVMD TEVYSNTGGQ SSKATPTGAV AKFAAAGKRT 

      1030       1040       1050       1060       1070       1080 
GKKDLARMVM TYGYVYVATV SMGYSKQQFL KVLKEAESFP GPSLVIAYAT CINQGLRKGM 

      1090       1100       1110       1120       1130       1140 
GKSQDVMNTA VKSGYWPLFR YDPRLAAQGK NPFQLDSKAP DGSVEEFLMA QNRFAVLDRS 

      1150       1160       1170       1180       1190       1200 
FPEDAKRLRA QVAHELDVRF KELEHMAATN IFESFAPAGG KADGSVDFGE GAEFCTRDDT 

      1210       1220       1230 
PMMARPDSGE ACDQNRAGTS EQQGDLSKRT KK

P94692 in FASTA format

View entry in original UniProtKB/TrEMBL format
View entry in raw text format (no links)
Request for annotation of this UniProtKB/TrEMBL entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools