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UniProtKB/Swiss-Prot entry P93832


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU32_ARATH
Primary accession number P93832
Secondary accession numbers Q540Z4 Q8W4P6
Integrated into Swiss-Prot on July 11, 2002
Sequence was last modified on May 1, 1997 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 63)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase 2, chloroplastic [Precursor]
Synonyms 3-IPM-DH 2
IMDH 2
EC 1.1.1.85
Beta-IPM dehydrogenase 2
Gene name
Name: IMDH2
Synonyms: IMDH
OrderedLocusNames: At1g80560
ORFNames: T21F11.11
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
Salchert K.D.;
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Y10216; CAA71268.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC018849; AAF27137.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY062441; AAL32519.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY114627; AAM47946.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR F96837; F96837.
RefSeq NP_178171.1; -.
UniGene At.5371
3D structure databases
HSSP Q56268; 1A05. [HSSP ENTRY / PDB]
ModBase P93832.
Organism-specific databases
TAIR At1g80560; -.
Gene expression databases
ArrayExpress P93832; -.
GermOnline AT1G80560; Arabidopsis thaliana.
Family and domain databases
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS P93832.
Genome annotation databases
GeneID 844395; -.
GenomeReviews CT485782_GR; AT1G80560.
KEGG ath:AT1G80560; -.
NMPDR fig|3702.1.peg.7645; -.
Other
ProtoNet P93832.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Chloroplast; Complete proteome; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    33  33     Chloroplast (Potential). 
CHAIN   34   405  372     3-isopropylmalate dehydrogenase 2, chloroplastic. PRO_0000014454
NP_BIND   116   129  14     NAD (By similarity). 
NP_BIND   322   334  13     NAD (By similarity). 
METAL   264   264        Magnesium or manganese (By similarity). 
METAL   288   288        Magnesium or manganese (By similarity). 
METAL   292   292        Magnesium or manganese (By similarity). 
BINDING   136   136        Substrate (By similarity). 
BINDING   146   146        Substrate (By similarity). 
BINDING   174   174        Substrate (By similarity). 
BINDING   264   264        Substrate (By similarity). 
SITE   181   181  1     Important for catalysis (By similarity). 
SITE   232   232  1     Important for catalysis (By similarity). 
CONFLICT   177   177        T -> K (in Ref. 3; AAL32519/AAM47946). 
Sequence information
Length: 405 AA [This is the length of the unprocessed precursor] Molecular weight: 43371 Da [This is the MW of the unprocessed precursor] CRC64: D5E22968F19FC7DC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAAALQTNIR TVKVPATFRA VSKQSLAPFR VRCAVASPGK KRYTITLLPG DGIGPEVVSI 

        70         80         90        100        110        120 
AKNVLQQAGS LEGVEFNFRE MPIGGAALDL VGVPLPEETI SAAKESDAVL LGAIGGYKWD 

       130        140        150        160        170        180 
NNEKHLRPEK GLLQIRAALK VFANLRPATV LPQLVDASTL KREVAEGVDL MVVRELTGGI 

       190        200        210        220        230        240 
YFGEPRGIKT NENGEEVGFN TEVYAAHEID RIARVAFETA RKRRGKLCSV DKANVLEASI 

       250        260        270        280        290        300 
LWRKRVTALA SEYPDVELSH MYVDNAAMQL VRDPKQFDTI VTNNIFGDIL SDEASMITGS 

       310        320        330        340        350        360 
IGMLPSASLS DSGPGLFEPI HGSAPDIAGQ DKANPLATIL SAAMLLKYGL GEEKAAKRIE 

       370        380        390        400 
DAVLVALNNG FRTGDIYSAG TKLVGCKEMG EEVLKSVDSQ VPASV 

P93832 in FASTA format

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