[1]	NUCLEOTIDE SEQUENCE [MRNA]. Akashi T., Aoki T., Kameya N., Nakamura I., Ayabe S.; "Two new cytochrome P450 cDNAs from elicitor-induced Licorice (Glycyrrhiza echinata L.) cells."; (er) Plant Gene Register PGR97-167.
[2]	CHARACTERIZATION. DOI=10.1006/bbrc.1998.9414; PubMed=9790908 [NCBI, ExPASy, EBI, Israel, Japan] Akashi T., Aoki T., Ayabe S.; "CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase."; Biochem. Biophys. Res. Commun. 251:67-70(1998).

Comments

FUNCTION: Catalyzes the hydroxylation of isoflavones, daidzein and formononetin, to yield 2'-hydroxyisoflavones, 2'-hydroxydaidzein, and 2'-hydroxyformononetin, respectively.
CATALYTIC ACTIVITY: An isoflavone + NADPH + O₂ = a 2'-hydroxyisoflavone + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Membrane (Potential).
INDUCTION: By fungal elicitor.
MISCELLANEOUS: Involved in the biosynthesis of isoflavonoid-derived antimicrobial compounds of legumes.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB001379; BAA22422.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P93147.

Ontologies

GO:0033773;	Molecular function: isoflavone 2'-hydroxylase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 499`	`499`	`Cytochrome P450 81E1.`	`PRO_0000052160`
`METAL`	`436 436`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 499 AA [This is the length of the unprocessed precursor]

Molecular weight: 57192 Da [This is the MW of the unprocessed precursor]

CRC64: 4D03AFAC818C04DB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEILSLLSYS VFYLALFFIF NIVIRARKFK NLPPGPPSLP IIGNLHHLKR PLHRTFKGLS 

        70         80         90        100        110        120 
EKYGHVFSLW FGSRLVVVVS SASEFQQCFT KNDVVLANRP RFLSGKYIFY NYTTLGSTSY 

       130        140        150        160        170        180 
GEHWRNLRRI TALDVLSNHR INSFSGIRRD ETQRLITRLA DDSSTNFAEM ELSSRLYDMT 

       190        200        210        220        230        240 
FNNIMRMISG KRYYGEDCDT SDLQEASQFR DMVSELLQLS GANNKTDFMP LLRFLDFENL 

       250        260        270        280        290        300 
EKRLKDISGK TDAFLRGLIE EHRTKKERAN TMIDHLLNLQ DSQPEYYTDQ IIKGLALAML 

       310        320        330        340        350        360 
LAGTDSSAVT LEWSMSNLLN HPEVLKKVKD ELDTHVGQDR LVDESDLPKL TYLKNVINET 

       370        380        390        400        410        420 
LRLYTPAPLL LPHSTSDECN IGGYKVPQDT IVLINAWAIH RDPELWTEAT TFKPERFEKK 

       430        440        450        460        470        480 
GELEKLIAFG MGRRACPGEG LAIRAISMTL ALLIQCFDWK LINGDKIDLA ERDGFTLTKL 

       490 
VPLKAMCKSR PVINKVFKQ

P93147 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools