ID   GPDA_TRYBB              Reviewed;         354 AA.
AC   P90593;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-NOV-2008, entry version 51.
DE   RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal;
DE            EC=1.1.1.8;
GN   Name=GPD;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=427;
RX   MEDLINE=97077437; PubMed=8920004; DOI=10.1016/0166-6851(95)02556-1;
RA   Kohl L., Drmota T., Thi C.-D., Callens M., van Beeumen J.,
RA   Opperdoes F.R., Michels P.A.M.;
RT   "Cloning and characterization of the NAD-linked glycerol-3-phosphate
RT   dehydrogenases of Trypanosoma brucei brucei and Leishmania mexicana
RT   mexicana and expression of the trypanosome enzyme in Escherichia
RT   coli.";
RL   Mol. Biochem. Parasitol. 76:159-173(1996).
CC   -!- CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(+) = glycerone
CC       phosphate + NADH.
CC   -!- SUBCELLULAR LOCATION: Glycosome.
CC   -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; X89738; CAA61890.1; -; Genomic_DNA.
DR   PIR; T48649; T48649.
DR   HSSP; P90551; 1EVY.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0020015; C:glycosome; IEA:UniProtKB-KW.
DR   GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase (NAD+) a...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013328; DHase_multihelical.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   InterPro; IPR017751; NAD-dep_Gly3P_DH_euk.
DR   InterPro; IPR006168; NAD-dep_Gly3P_DHase.
DR   InterPro; IPR011128; NAD-dep_Gly3P_DHase_N.
DR   InterPro; IPR006109; NAD_Gly3P_DHase_C.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR11728; NAD_Gly3P_DH; 1.
DR   Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR   Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR   PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR   PRINTS; PR00077; GPDHDRGNASE.
DR   ProDom; PD001278; NAD_Gly3P_C; 1.
DR   PROSITE; PS00957; NAD_G3PDH; 1.
PE   3: Inferred from homology;
KW   Glycosome; NAD; Oxidoreductase.
FT   CHAIN         1    354       Glycerol-3-phosphate dehydrogenase
FT                                [NAD+], glycosomal.
FT                                /FTId=PRO_0000138083.
FT   NP_BIND      15     20       NAD (By similarity).
FT   REGION      267    268       Substrate binding (By similarity).
FT   MOTIF       352    354       Microbody targeting signal (Potential).
FT   ACT_SITE    203    203       Proton acceptor (By similarity).
FT   BINDING      90     90       NAD (By similarity).
FT   BINDING     118    118       NAD; via amide nitrogen (By similarity).
FT   BINDING     118    118       Substrate (By similarity).
FT   BINDING     150    150       NAD; via amide nitrogen (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     293    293       NAD (By similarity).
SQ   SEQUENCE   354 AA;  37783 MW;  12CE19AEDA9E4EC9 CRC64;
     MVSGVTYLKR GAVFGSGAFG TALACVLAKK CESVSVWHMN ANEARVVNQK HENVYFLPGA
     PLPANLTFTA DAEECAKGAE IVLFVIPTQF LRGFLQKNSH ILRNHVVSRN VPVVMCSKGI
     ERSSLLFPAQ ILEEFLPNYP IGVIAGPSFA IEVAKGMLTN VCTAAADINM ARKIQRIMTT
     SDGSFRCWAT TDVIGCEIAS AMKNVLAIAS GALKGLGTEN NARAALISRG LLEIRDLTLA
     LGGTGEAVFG LPGLGDLLLT CSSELSRNFT VGMKLGQGIS LEEIKRTSKA VAEGVATAEP
     LERLAKKHNA DLPICHEVYN VLYANGCAKR SFKKLNSCKL ADEGLPALPR TSKM
//