NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=A733;
DOI=10.1007/s002940050137; PubMed=8781173 [NCBI, ExPASy, EBI, Israel, Japan]
Williams B.A., Sillaots S., Tsang A., Storms R.;
"Isolation by genetic complementation of two differentially expressed genes for beta-isopropylmalate dehydrogenase from Aspergillus niger.";
Curr. Genet. 30:305-311(1996).

Comments

FUNCTION: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
CATALYTIC ACTIVITY: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD⁺ = 3-carboxy-4-methyl-2-oxopentanoate + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S83228; AAB50827.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U51130; AAD10278.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S72209; S72209.

3D structure databases

HSSP

P12010; 2AYQ. [HSSP ENTRY / PDB]

ModBase

P87256.

Family and domain databases

InterPro

IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00169; leuB; 1.

PROSITE

PS00470; IDH_IMDH; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 363`	`363`	`3-isopropylmalate dehydrogenase A.`	`PRO_0000083599`
`NP_BIND`	`78 89`	`12`	`NAD (By similarity).`
`NP_BIND`	`287 299`	`13`	`NAD (By similarity).`
`METAL`	`222 222`		`Magnesium or manganese (By similarity).`
`METAL`	`247 247`		`Magnesium or manganese (By similarity).`
`METAL`	`251 251`		`Magnesium or manganese (By similarity).`
`BINDING`	`96 96`		`Substrate (By similarity).`
`BINDING`	`106 106`		`Substrate (By similarity).`
`BINDING`	`135 135`		`Substrate (By similarity).`
`BINDING`	`222 222`		`Substrate (By similarity).`
`SITE`	`142 142`	`1`	`Important for catalysis (By similarity).`
`SITE`	`189 189`	`1`	`Important for catalysis (By similarity).`

Sequence information

Length: 363 AA [This is the length of the unprocessed precursor]

Molecular weight: 38670 Da [This is the MW of the unprocessed precursor]

CRC64: 20AC729774C646B7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPAYNIVVFA GDHWGPEVTA EAIKVLRVIE KSRDDITLNL QDHLLGGASI DATANPLTDE 

        70         80         90        100        110        120 
ALAAAKNADA VLLGAIGGPK WGTGAVRPEQ GLLNVRKEMG TFGNLRPCNF AAPSLVEHSP 

       130        140        150        160        170        180 
LKASVCEGVD FNIIRELTGG IYFGDRKKMT AAATMDTEPY SRAEIERITP RAHLALQHNP 

       190        200        210        220        230        240 
PLPVWSLDKA NVLATSRLWR KTVTEIMAKE FPQLKIEHQL IDSAAMIMVK NPRQLNGIIV 

       250        260        270        280        290        300 
TSNLFGDIIS DEASVIPGSL GLLPSASLSG IPDGKGRVNG IYEPIHGSAP DIAGKGIVNP 

       310        320        330        340        350        360 
VAAILSVAMM MQYSFGRFDE ARAIEAAVRN VLESGVRTGD IGGKATTSEV GDAVAAELEK 


LLK

P87256 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools