ID   LAC1B_CERUI             Reviewed;           9 AA.
AC   P85308;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   04-NOV-2008, entry version 5.
DE   RecName: Full=Laccase-1b;
DE            EC=1.10.3.2;
DE   AltName: Full=Benzenediol:oxygen oxidoreductase 1b;
DE   AltName: Full=Urishiol oxidase 1b;
DE   AltName: Full=Diphenol oxidase 1b;
DE   AltName: Full=Lac Ib;
DE   Flags: Fragment;
OS   Cerrena unicolor (Canker rot fungus) (Daedalea unicolor).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
OC   Agaricomycetes; Polyporales; Cerrena.
OX   NCBI_TaxID=90312;
RN   [1]
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL
RP   PROPERTIES.
RA   Checinska A., Janusz G., Rogalski J.M.;
RL   Submitted (OCT-2007) to UniProtKB.
CC   -!- FUNCTION: Lignin degradation and detoxification of lignin-derived
CC       products (Probable).
CC   -!- CATALYTIC ACTIVITY: 4 benzenediol + O(2) = 4 benzosemiquinone + 2
CC       H(2)O.
CC   -!- COFACTOR: Binds 4 copper ions per monomer (By similarity).
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.153 mM for syringaldazine;
CC       pH dependence:
CC         Optimum pH is 5.3;
CC       Temperature dependence:
CC         Optimum temperature is 60 degrees Celsius;
CC   -!- SUBCELLULAR LOCATION: Secreted (By similarity).
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is:
CC       4.27, its MW is: 54.49 kDa.
CC   -!- SIMILARITY: Belongs to the multicopper oxidase family.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0005507; F:copper ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008471; F:laccase activity; IEA:EC.
DR   GO; GO:0046274; P:lignin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002355; Cu_oxidase_Cu_BS.
DR   PROSITE; PS00079; MULTICOPPER_OXIDASE1; PARTIAL.
DR   PROSITE; PS00080; MULTICOPPER_OXIDASE2; PARTIAL.
PE   1: Evidence at protein level;
KW   Copper; Direct protein sequencing; Lignin degradation; Metal-binding;
KW   Oxidoreductase; Secreted.
FT   CHAIN         1     >9       Laccase-1b.
FT                                /FTId=PRO_0000310833.
FT   NON_TER       9      9
SQ   SEQUENCE   9 AA;  892 MW;  1CE22AADC2D76870 CRC64;
     AIGPVADLH
//