NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
DOI=10.1006/expr.1997.4165; PubMed=9225772 [NCBI, ExPASy, EBI, Israel, Japan]
Hagen H.-E., Klager S.L., McKerrow J.H., Ham P.J.;
"Simulium damnosum s.l.: isolation and identification of prophenoloxidase following an infection with Onchocerca spp. using targeted differential display.";
Exp. Parasitol. 86:213-218(1997).

Comments

FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone.
CATALYTIC ACTIVITY: L-tyrosine + L-dopa + O₂ = L-dopa + dopaquinone + H₂O.
COFACTOR: Binds 2 copper ions per subunit (By similarity).
SUBUNIT: Heterodimer (By similarity).
SUBCELLULAR LOCATION: Secreted (By similarity).
INDUCTION: By infection with O.dukei and O.ochengi.
SIMILARITY: Belongs to the tyrosinase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

3D structure databases

ModBase

P85046.

Ontologies

GO:0005576;	Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0005507;	Molecular function: copper ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004503;	Molecular function: monophenol monooxygenase activity (inferred from electronic annotation from EC).
GO:0005344;	Molecular function: oxygen transporter activity (inferred from electronic annotation from InterPro).
GO:0006583;	Biological process: melanin biosynthetic process from tyrosine (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006810;	Biological process: transport (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR008922; Di-copper_centre.
IPR013788; Hemocyanin.
IPR005203; Hemocyanin_C.
IPR000896; Hemocyanin_Cu.
IPR002227; Tyrosinase.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.1280.10; Di-copper_centre; 1.
G3DSA:2.60.40.1520; hemocyanin_C; 1.

PANTHER

PTHR11511; Hemocyanin; 1.

Pfam

PF03723; Hemocyanin_C; 1.
PF00372; Hemocyanin_M; 1.
Pfam graphical view of domain structure.

PROSITE

PS00209; HEMOCYANIN_1; PARTIAL.
PS00210; HEMOCYANIN_2; PARTIAL.
PS00497; TYROSINASE_1; PARTIAL.
PS00498; TYROSINASE_2; 1.

ProtoNet

P85046.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Copper; Glycoprotein; Melanin biosynthesis; Metal-binding; Monooxygenase; Oxidoreductase; Secreted.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 >195`	`>195`	`Phenoloxidase subunit 1.`	`PRO_0000271231`
`METAL`	`10 10`		`Copper B (By similarity).`
`CARBOHYD`	`77 77`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`97 97`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`98 98`		`N-linked (GlcNAc...) (Potential).`
`NON_TER`	`1 1`
`NON_TER`	`195 195`

Sequence information

Length: 195 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 22867 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 137ECB8E37454C4F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRDPFFYRWH SYIDDIFQEH KERLRPYTEA QLNFNGITVT GVQVAPERGP TNTFQTSWQQ 

        70         80         90        100        110        120 
SDVDLSRGMD FVAPRGNVTA RFTHLNHTPF TYSIQVNNSS GAQRMGMVRI FLAPKTDERG 

       130        140        150        160        170        180 
NEMLFRDQRL MMIEMDKFVV SMRPGQNTIR RRSTESTVTI PFERTFRSLE ESRPDQTTDA 

       190 
QQQFNFCGCG WPHHM

P85046 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools