PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND GLYCOSYLATION.
TISSUE=Rhizome;
Joseph A., Thayumanavan B., Manickam A., Panicker P.R.;
"Purification and characterization of a polyphenol oxidase from the rhizome of Zingiber officinale.";
Submitted (SEP-2006) to UniProtKB.

Comments

FUNCTION: Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.
CATALYTIC ACTIVITY: 2 catechol + O₂ = 2 1,2-benzoquinone + 2 H₂O.
COFACTOR: Binds 2 copper ions per subunit (By similarity).

BIOPHYSICOCHEMICAL PROPERTIES:

pH dependence:	Optimum pH is 4.5. Active from pH 3.0 to 8.0. The activity decreases sharply above pH 7.5;
Temperature dependence:	Optimum temperature is 60 degrees Celsius. There is only a slight decrease in activity at 75 degrees Celsius and a sharp decrease in activity at 90 degrees Celsius;

SUBUNIT: Homodimer.
TISSUE SPECIFICITY: Expressed in the rhizome. Not detected in leaves.
DEVELOPMENTAL STAGE: Expressed throughout rhizome development.
PTM: Glycosylated.
SIMILARITY: Belongs to the tyrosinase family.

Copyright

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Cross-references

3D structure databases
ModBase	P85026.
Family and domain databases
InterPro	IPR002227; Tyrosinase. Graphical view of domain structure.
PROSITE	PS00497; TYROSINASE_1; PARTIAL. PS00498; TYROSINASE_2; PARTIAL.
BLOCKS	P85026.
Other
ProtoNet	P85026.
UniRef	View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Copper; Direct protein sequencing; Metal-binding; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 >10`	`>10`	`Polyphenol oxidase.`	`PRO_0000259621`
`NON_TER`	`10 10`

Sequence information

Length: 10 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 946 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 38286BAAA87862C8 [This is a checksum on the sequence]

        10 
EQGVGGDDGL

P85026 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools