ID   G3P3_KLUMA              Reviewed;         331 AA.
AC   P84999;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-NOV-2008, entry version 17.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 3;
DE            Short=GAPDH 3;
DE            EC=1.2.1.12;
GN   Name=GAP3;
OS   Kluyveromyces marxianus (Yeast) (Candida kefyr).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=4911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, AND PATHWAY.
RC   STRAIN=ATCC 10022 / CBS 6432 / NCTC 2303 / NRRL Y-665;
RX   MEDLINE=95397590; PubMed=7668042;
RA   Fernandes P.A., Sena-Esteves M., Moradas-Ferreira P.;
RT   "Characterization of the glyceraldehyde-3-phosphate dehydrogenase gene
RT   family from Kluyveromyces marxianus -- polymerase chain reaction-
RT   single-strand conformation polymorphism as a tool for the study of
RT   multigenic families.";
RL   Yeast 11:725-733(1995).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   PIR; S57281; S57281.
DR   SMR; P84999; 1-329.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DHase.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DHase_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase; Phosphoprotein.
FT   CHAIN         1    331       Glyceraldehyde-3-phosphate dehydrogenase
FT                                3.
FT                                /FTId=PRO_0000253991.
FT   NP_BIND      11     12       NAD (By similarity).
FT   REGION      148    150       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      208    209       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    149    149       Nucleophile (By similarity).
FT   BINDING      33     33       NAD (By similarity).
FT   BINDING      77     77       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     179    179       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     231    231       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     313    313       NAD (By similarity).
FT   SITE        176    176       Activates thiol group during catalysis
FT                                (By similarity).
FT   MOD_RES     148    148       Phosphoserine (By similarity).
FT   MOD_RES     177    177       Phosphoserine (By similarity).
FT   MOD_RES     200    200       Phosphoserine (By similarity).
SQ   SEQUENCE   331 AA;  35524 MW;  5E09084264795F01 CRC64;
     MVRIAINGFG RIGRLVLRIA LSRKNIEVVA INDPFITVDY AAYMFKYDST HGRFDGEVSH
     DGKALIIDGK KVLVFQERDP ATLPWGAEKI DIAIDSTGIF KELDSAQKHI DAGAKKVVIT
     APSSTAPMFV VGVNEDKYAG QTIVSNASCT TNCLAPLAKI INNAFGIEEG LMTTVHSITA
     TQKTVDGPSH KDWRGGRTAS GNIIPSSTGA AKAVGKVLPE LQGKLTGMAF RVPTVDVSVV
     DLTVKLAKPA TYEEIKAVVK KASENELKGV MGYTEDAVVS SDFLGDTHSS IFDAAAGIQL
     SPQFVKLVSW YDNEFGYSTR VVDLVELVAK N
//