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UniProtKB/Swiss-Prot entry P82281


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name APX4_ARATH
Primary accession number P82281
Secondary accession numbers Q7XZP6 Q9M0S6
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on December 1, 2000 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 58)
Name and origin of the protein
Protein name Putative L-ascorbate peroxidase 4, chloroplastic [Precursor]
Synonyms EC 1.11.1.11
Thylakoid lumenal 29 kDa protein
TL29
P29
AtAPx07
Gene name
Name: APX4
OrderedLocusNames: At4g09010
ORFNames: F23J3.40
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/47134; PubMed=10617198 [NCBI, ExPASy, EBI, Israel, Japan]
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.;
"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
Nature 402:769-777(1999).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[3]
NUCLEOTIDE SEQUENCE [MRNA] OF 11-306.
STRAIN=cv. Columbia;
TISSUE=Leaf;
DOI=10.1104/pp.001362; PubMed=12068123 [NCBI, ExPASy, EBI, Israel, Japan]
Panchuk I.I., Volkov R.A., Schoffl F.;
"Heat stress- and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis.";
Plant Physiol. 129:838-853(2002).
[4]
PROTEIN SEQUENCE OF 83-112, AND SUBCELLULAR LOCATION.
STRAIN=cv. Columbia;
DOI=10.1016/S0014-5793(00)01890-1; PubMed=11034343 [NCBI, ExPASy, EBI, Israel, Japan]
Kieselbach T., Bystedt M., Hynds P., Robinson C., Schroeder W.P.;
"A peroxidase homologue and novel plastocyanin located by proteomics to the Arabidopsis chloroplast thylakoid lumen.";
FEBS Lett. 480:271-276(2000).
[5]
DEVELOPMENTAL STAGE.
DOI=10.1007/s00425-005-0028-8; PubMed=16034597 [NCBI, ExPASy, EBI, Israel, Japan]
Panchuk I.I., Zentgraf U., Volkov R.A.;
"Expression of the Apx gene family during leaf senescence of Arabidopsis thaliana.";
Planta 222:926-932(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AC005359; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
AL161513; CAB78025.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF370534; AAK48961.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY072503; AAL66918.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF441713; AAP72143.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A85091; A85091.
RefSeq NP_192640.1; -.
UniGene At.22637
3D structure databases
HSSP P48534; 1APX. [HSSP ENTRY / PDB]
ModBase P82281.
Protein family/group databases
PeroxiBase 3920; AtAPx07.
Organism-specific databases
GeneFarm 1959; 146.
TAIR At4g09010; -.
Gene expression databases
GermOnline AT4G09010; Arabidopsis thaliana.
Ontologies
GO
GO:0016688; Molecular function: L-ascorbate peroxidase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR002207; Asc_perxdse.
IPR002016; Haem_peroxidase_pln/fun/bac.
Graphical view of domain structure.
Pfam PF00141; peroxidase; 1.
Pfam graphical view of domain structure.
PRINTS PR00459; ASPEROXIDASE.
PR00458; PEROXIDASE.
PROSITE PS00435; PEROXIDASE_1; FALSE_NEG.
PS00436; PEROXIDASE_2; FALSE_NEG.
PS50873; PEROXIDASE_4; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P82281.
Genome annotation databases
GeneID 826480; -.
GenomeReviews CT486007_GR; AT4G09010.
KEGG ath:AT4G09010; -.
NMPDR fig|3702.1.peg.18558; -.
Other
ProtoNet P82281.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Chloroplast; Complete proteome; Direct protein sequencing; Hydrogen peroxide; Oxidoreductase; Peroxidase; Plastid; Thylakoid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
TRANSIT   1     ?        Chloroplast (Potential). 
TRANSIT   ?    82        Thylakoid. 
CHAIN   83   349  267     Putative L-ascorbate peroxidase 4, chloroplastic. PRO_0000023635
CONFLICT   96    96        S -> A (in Ref. 3; AAP72143). 
Sequence information
Length: 349 AA [This is the length of the unprocessed precursor] Molecular weight: 37934 Da [This is the MW of the unprocessed precursor] CRC64: ABD439188BDA8E61 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGGVSFLSTV PSFTNTTNHQ HLTTLSSSSH RSAVIRCSKI EPQVSGESLA FHRRDVLKLA 

        70         80         90        100        110        120 
GTAVGMELIG NGFINNVGDA KAADLNQRRQ RSEFQSKIKI LLSTTIKAKP ELVPSLLKLA 

       130        140        150        160        170        180 
LNDAMTYDKA TKSGGANGSI RFSSELSRAE NEGLSDGLSL IEEVKKEIDS ISKGGPISYA 

       190        200        210        220        230        240 
DIIQLAGQSA VKFTYLASAI RKCGGNEEKG NLLYTAYGSA GQWGLFDRNF GRSDATEADP 

       250        260        270        280        290        300 
EGRVPQWGKA TVQEMKDKFI AVGLGPRQLA VMSAFLGPDQ AATEQLLATD PQVAPWVQKY 

       310        320        330        340 
QRSRETVSQT DYEVDLITAF TKLSCLGQQI NFEAYTYPVE RINLSKLKL 

P82281 in FASTA format

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