[1]	NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION. DOI=10.1074/jbc.M005103200; PubMed=10915798 [NCBI, ExPASy, EBI, Israel, Japan] Taniguchi N., Taniura H., Niinobe M., Takayama C., Tominaga-Yoshino K., Ogura A., Yoshikawa K.; "The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm."; J. Biol. Chem. 275:31674-31681(2000).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=Swiss Webster / NIH; TISSUE=Embryo; Hoefig K., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N.; Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=FVB/N; TISSUE=Mammary tumor; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Calcium-binding protein. May have a role in calcium homeostasis.
SUBUNIT: Binds to the postmitotic growth suppressor necdin; co-expression abolishes Nucb2 secretion.
SUBCELLULAR LOCATION: Perikaryon. Endoplasmic reticulum. Nucleus envelope. Note=In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm. In cell culture, cytoplasmic and secreted.
TISSUE SPECIFICITY: Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons.
SIMILARITY: Belongs to the nucleobindin family.
SIMILARITY: Contains 2 EF-hand domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ222586; CAA10858.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC010459; AAH10459.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001123951.1; -.

UniGene

Mm.9901

3D structure databases

P81117; 229-327.

PTM databases

P81117; -.

Organism-specific databases

MGI

MGI:1858179; Nucb2.

Gene expression databases

ArrayExpress

P81117; -.

CleanEx

MM_NUCB2; -.

GermOnline

ENSMUSG00000030659; Mus musculus.

Ontologies

GO:0005783;	Cellular component: endoplasmic reticulum (inferred from direct assay from MGI).
GO:0005640;	Cellular component: nuclear outer membrane (inferred from direct assay from MGI).
GO:0005509;	Molecular function: calcium ion binding (inferred from direct assay from MGI).
GO:0006874;	Biological process: cellular calcium ion homeostasis (traceable author statement from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR011992; EF-Hand_type.
IPR002048; EF_hand_Ca_bd.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.238.10; EF-Hand_type; 1.

Pfam

PF00036; efhand; 2.
Pfam graphical view of domain structure.

SMART

SM00054; EFh; 2.
SMART graphical view of domain structure.

PROSITE

PS00018; EF_HAND_1; 2.
PS50222; EF_HAND_2; 2.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P81117.

Genome annotation databases

Ensembl

ENSMUSG00000030659; Mus musculus. [Contig view]

GeneID

53322; -.

Phylogenomic databases

HOGENOM

P81117; -.

HOVERGEN

P81117; -.

Other

Nucb2; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; DNA-binding; Endoplasmic reticulum; Nucleus; Repeat; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 24`	`24`	`Potential.`
`CHAIN`	`25 420`	`396`	`Nucleobindin-2.`	`PRO_0000004166`
`DOMAIN`	`241 276`	`36`	`EF-hand 1.`
`DOMAIN`	`293 328`	`36`	`EF-hand 2.`
`DNA_BIND`	`171 223`	`53`	`By similarity.`
`CA_BIND`	`254 265`	`12`	`1.`
`CA_BIND`	`306 317`	`12`	`2.`
`REGION`	`213 420`	`208`	`Binds to necdin.`
`COMPBIAS`	`284 293`	`10`	`Asp/Glu-rich (acidic).`
`CONFLICT`	`18 18`		`M -> T (in Ref. 2; CAA10858).`

Sequence information

Length: 420 AA [This is the length of the unprocessed precursor]

Molecular weight: 50304 Da [This is the MW of the unprocessed precursor]

CRC64: 0DEBD51724CB7E72 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT GLYYDEYLKQ 

        70         80         90        100        110        120 
VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA 

       130        140        150        160        170        180 
KLDALQDTGM NHHLLLKQFE HLNHQNPNTF ESRDLDMLIK AATADLEQYD RTRHEEFKKY 

       190        200        210        220        230        240 
EMMKEHERRE YLKTLSEEKR KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD 

       250        260        270        280        290        300 
PNDFDPKTFF KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH 

       310        320        330        340        350        360 
VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY ESIIAIQENE 

       370        380        390        400        410        420 
LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK LQQGIAPSGP AGELKFEPHT

P81117 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools