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UniProtKB/Swiss-Prot entry P80686


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CUH1C_TENMO
Primary accession number P80686
Secondary accession number O18509
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on July 15, 1998 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 41)
Name and origin of the protein
Protein name Larval/pupal cuticle protein H1C [Precursor]
Synonyms TM-PCP H1C
TM-H1C
TMLPCP-22
Gene name
Name: LPCP-22
From
Tenebrio molitor (Yellow mealworm) [TaxID: 7067] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Tenebrionidae; Tenebrio.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Epidermis;
PubMed=8631320 [NCBI, ExPASy, EBI, Israel, Japan]
Rondot I., Quennedey B., Courrent A., Lemoine A., Delachambre J.;
"Cloning and sequencing of a cDNA encoding a larval-pupal-specific cuticular protein in Tenebrio molitor (Insecta, Coleoptera). Developmental expression and effect of a juvenile hormone analogue.";
Eur. J. Biochem. 235:138-143(1996).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=9660184 [NCBI, ExPASy, EBI, Israel, Japan]
Rondot I., Quennedey B., Delachambre J.;
"Structure, organization and expression of two clustered cuticle protein genes during the metamorphosis of an insect, Tenebrio molitor.";
Eur. J. Biochem. 254:304-312(1998).
[3]
PROTEIN SEQUENCE OF 17-211, AND MASS SPECTROMETRY.
TISSUE=Cuticle;
DOI=10.1016/S0965-1748(96)00076-8; PubMed=9066122 [NCBI, ExPASy, EBI, Israel, Japan]
Andersen S.O., Rafn K., Roepstorff P.;
"Sequence studies of proteins from larval and pupal cuticle of the yellow meal worm, Tenebrio molitor.";
Insect Biochem. Mol. Biol. 27:121-131(1997).
Comments
  • FUNCTION: Component of the hard and soft cuticles of the larva and pupa of Tenebrio molitor.
  • TISSUE SPECIFICITY: Restricted to sternal, tergal, legs and wing anlagen epidermis, and soft cuticle secreting epidermis, such as intersegmental membranes and pleura. No expression in muscles or fat body.
  • DEVELOPMENTAL STAGE: Expressed in epidermal cells during the secretion of preecdysial larval and pupal cuticles. Restricted to a short part of the intersegmental regions after pupal ecdysis.
  • INDUCTION: By juvenile hormone (Probable).
  • DOMAIN: The tetrapeptide (A-A-P-[AV]) repeats found throughout the protein are also present in many proteins constituting the protective envelope of other species.
  • MASS SPECTROMETRY: Mass=19182.7; Method=Electrospray; Range=17-211; Source=PubMed:9066122;.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X87983; CAA61238.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y09124; CAA70342.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S62598; S62598.
T12013; T12013.
3D structure databases
ModBase P80686.
Ontologies
GO
GO:0042302; Molecular function: structural constituent of cuticle (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Other
ProtoNet P80686.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cuticle; Direct protein sequencing; Repeat; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    16  16      
CHAIN   17   211  195     Larval/pupal cuticle protein H1C. PRO_0000006417
REPEAT   29    32  4     1-1. 
REPEAT   41    44  4     1-2. 
REPEAT   128   137  10     2-1. 
REPEAT   131   134  4     1-3. 
REPEAT   138   147  10     2-2. 
REPEAT   141   144  4     1-4. 
REPEAT   148   156  9     3-1. 
REPEAT   157   165  9     3-2. 
REPEAT   166   174  9     3-3. 
REPEAT   175   183  9     3-4. 
REGION   128   147  20     2 X 10 AA tandem repeat of Y-A-H-A-A-P-V-V-S-[ST]. 
REGION   148   183  36     4 X 9 AA tandem repeat of Y-A-A-H-A-P-V-[VA]-[AT]. 
CONFLICT   156   156        A -> T (in Ref. 3; AA sequence). 
CONFLICT   181   181        V -> VATYAAHAPV (in Ref. 1). 
Sequence information
Length: 211 AA [This is the length of the unprocessed precursor] Molecular weight: 20913 Da [This is the MW of the unprocessed precursor] CRC64: 2E21FAE609F4FE37 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MYKFVVFAAA LAYANAGLIG APAVAAYSAA PAVSSAYIHQ AAPVAVAHAA PLAVAHAPVA 

        70         80         90        100        110        120 
VAHAAPYAIH APAVGASHQS VVRSLGGNQA VSHYSKAVDS AFSSVRKFDT RVTNDALAVA 

       130        140        150        160        170        180 
HAPVVSTYAH AAPVVSTYAH AAPVVSSYAA HAPVAAYAAH APVATYAAHA PVATYAAHAP 

       190        200        210 
VVATRTSAVA YSPAAVVSHA SFSGLGASYA W 

P80686 in FASTA format

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