[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1007/s002030050561; PubMed=9477253 [NCBI, ExPASy, EBI, Israel, Japan] Vaupel M., Thauer R.K.; "Two F420-reducing hydrogenases in Methanosarcina barkeri."; Arch. Microbiol. 169:201-205(1998).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1128/JB.00810-06; PubMed=16980466 [NCBI, ExPASy, EBI, Israel, Japan] Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.; "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."; J. Bacteriol. 188:7922-7931(2006).
[3]	PROTEIN SEQUENCE OF 2-20. PubMed=8521835 [NCBI, ExPASy, EBI, Israel, Japan] Michel R., Massanz C., Kostka S., Richter M., Fiebig K.; "Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro."; Eur. J. Biochem. 233:727-735(1995).

Comments

FUNCTION: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
CATALYTIC ACTIVITY: H₂ + coenzyme F420 = reduced coenzyme F420.
COFACTOR: Nickel.
COFACTOR: Iron-sulfur.
COFACTOR: FAD.
SUBUNIT: Pentamer of two alpha chains, two beta chains and a gamma chain.
SIMILARITY: Belongs to the frhG family.
SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Y13763; CAA74092.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CP000099; AAZ69432.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S63485; S63485.

RefSeq

YP_304012.1; -.

3D structure databases

ModBase

P80491.

Enzyme and pathway databases

BioCyc

MBAR269797:MBAR_A0450-MON; -.
MetaCyc:MON-12649; -.

Family and domain databases

InterPro

IPR001450; 4Fe4S_Fe_S_bd.
IPR017681; Coenz_F4210_hydrogenase_gsu.
IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.

PANTHER

PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.

Pfam

PF00037; Fer4; 2.
PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

PRINTS

PR00353; 4FE4SFRDOXIN.

PROSITE

PS00198; 4FE4S_FER_1; 2.
PS51379; 4FE4S_FER_2; 2.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P80491.

Genome annotation databases

GeneID

3624326; -.

GenomeReviews

CP000099_GR; Mbar_A0450.

KEGG

mba:Mbar_A0450; -.

NMPDR

fig|269797.3.peg.628; -.

Phylogenomic databases

HOGENOM

P80491; -.

Genome annotation databases

CMR

P80491; Mbar_A0450.

Other

ProtoNet

P80491.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Repeat; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 259`	`258`	`Coenzyme F420 hydrogenase subunit gamma.`	`PRO_0000159231`
`DOMAIN`	`180 208`	`29`	`4Fe-4S ferredoxin-type 1.`
`DOMAIN`	`209 238`	`30`	`4Fe-4S ferredoxin-type 2.`
`METAL`	`189 189`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`192 192`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`195 195`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`199 199`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`218 218`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`221 221`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`224 224`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`228 228`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`

Sequence information

Length: 259 AA [This is the length of the unprocessed precursor]

Molecular weight: 28241 Da [This is the MW of the unprocessed precursor]

CRC64: 46C4AE265B6146BF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTNKIKIGHV HMSGCTGCLV SLADNNLGLI KILDDYADLV YCLTLADVRH IPEMDVALVE 

        70         80         90        100        110        120 
GSVCLQDHES VEDIKETRKK SKIVVALGSC ACYGNITRFS RGGQHNQPQH ESYLPIGDLI 

       130        140        150        160        170        180 
DVDVYIPGCP PSPELIRNVA VMAYLLLEGN EEQKELAGKY LKPLMDLAKR GTSGCFCDLM 

       190        200        210        220        230        240 
YDVINQGLCM GCGTCAASCP VHAITLEFGK PQGERDLCIK CGSCYGACPR SFFNLDVIPE 

       250 
FENINEIIAN ALKEGESDD

P80491 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools