[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1007/s002030050561; PubMed=9477253 [NCBI, ExPASy, EBI, Israel, Japan] Vaupel M., Thauer R.K.; "Two F420-reducing hydrogenases in Methanosarcina barkeri."; Arch. Microbiol. 169:201-205(1998).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1128/JB.00810-06; PubMed=16980466 [NCBI, ExPASy, EBI, Israel, Japan] Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.; "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."; J. Bacteriol. 188:7922-7931(2006).
[3]	PROTEIN SEQUENCE OF 2-21. PubMed=8521835 [NCBI, ExPASy, EBI, Israel, Japan] Michel R., Massanz C., Kostka S., Richter M., Fiebig K.; "Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro."; Eur. J. Biochem. 233:727-735(1995).

Comments

FUNCTION: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
CATALYTIC ACTIVITY: H₂ + coenzyme F420 = reduced coenzyme F420.
COFACTOR: Nickel.
COFACTOR: Iron-sulfur.
COFACTOR: FAD.
SUBUNIT: Pentamer of two alpha chains, two beta chains and a gamma chain.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Y13763; CAA74090.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CP000099; AAZ69434.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S63483; S63483.

RefSeq

YP_304014.1; -.

3D structure databases

ModBase

P80489.

Enzyme and pathway databases

BioCyc

MBAR269797:MBAR_A0452-MON; -.
MetaCyc:MON-12647; -.

Family and domain databases

InterPro

IPR017682; Coenz_F420_hydrogenase_asu.
IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

BLOCKS

P80489.

Genome annotation databases

GeneID

3624328; -.

GenomeReviews

CP000099_GR; Mbar_A0452.

KEGG

mba:Mbar_A0452; -.

NMPDR

fig|269797.3.peg.630; -.

Phylogenomic databases

HOGENOM

P80489; -.

Genome annotation databases

CMR

P80489; Mbar_A0452.

Other

ProtoNet

P80489.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Complete proteome; Direct protein sequencing; FAD; Membrane; Metal-binding; Nickel; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 456`	`455`	`Coenzyme F420 hydrogenase subunit alpha.`	`PRO_0000199721`
`METAL`	`63 63`		`Nickel (Potential).`
`METAL`	`66 66`		`Nickel (Potential).`
`METAL`	`432 432`		`Nickel (Potential).`
`METAL`	`435 435`		`Nickel (Potential).`

Sequence information

Length: 456 AA [This is the length of the unprocessed precursor]

Molecular weight: 50769 Da [This is the MW of the unprocessed precursor]

CRC64: EEFBD0E04511D667 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTKVVEISPT TRLEGHSKLT LKVDDQGIVE RGDWLSITPV RGIEKLAIGK TMEQVPKIAS 

        70         80         90        100        110        120 
RVCGICPIAH TLASTEAMEA SIGCEIPTDA KLLRTILHAA NRIHSIALHN ILILPDFYIP 

       130        140        150        160        170        180 
GTEKKFNLFA NEQPARSVMA RIVRIREIAQ TIGAIAGGEA IHPSNPRIGG MYYNVSPRAK 

       190        200        210        220        230        240 
QKMADLAKEG LVLVHEQMEF MFDVIRNMQN REFVEVAGKQ IPLPKKLGYH NQGVMATASM 

       250        260        270        280        290        300 
YGSSSLDDNP TWDFTRWKET RPWDWYMGEV TIDLEDPSYP IGGTTKIGTK ANPQMEACTG 

       310        320        330        340        350        360 
VPTYDGQPVE VGPRARLATF KNFDEKGTFA QHIARQMEYP DCCYTILRCL DNLNTSGKVL 

       370        380        390        400        410        420 
ADHIPQGDGS MGWAANEAPR GSNIHLARVK DGKVLWYDML VPTTWNFPTC SRALTGAPWQ 

       430        440        450 
IAEMVVRAYD PCVSCATHMI VVNEEEKIVT QKLMQW

P80489 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools