[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=8763933 [NCBI, ExPASy, EBI, Israel, Japan] Muro-Pastor M.I., Reyes J.C., Florencio F.J.; "The NADP+-isocitrate dehydrogenase gene (icd) is nitrogen regulated in cyanobacteria."; J. Bacteriol. 178:4070-4076(1996).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1093/dnares/3.3.109; PubMed=8905231 [NCBI, ExPASy, EBI, Israel, Japan] Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.; "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."; DNA Res. 3:109-136(1996).
[3]	PROTEIN SEQUENCE OF 1-20. PubMed=1730247 [NCBI, ExPASy, EBI, Israel, Japan] Muro-Pastor M.I., Florencio F.J.; "Purification and properties of NADP-isocitrate dehydrogenase from the unicellular cyanobacterium Synechocystis sp. PCC 6803."; Eur. J. Biochem. 203:99-105(1992).

Comments

CATALYTIC ACTIVITY: Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
CATALYTIC ACTIVITY: Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
ENZYME REGULATION: Inhibition of this enzyme by phosphorylation regulates the branch point between the Krebs cycle and the glyoxylate bypass, which is an alternate route that accumulates carbon for biosynthesis when acetate is the sole carbon source for growth.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X83563; CAA58549.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000022; BAA16835.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S74684; S74684.

RefSeq

NP_440155.1; -.

3D structure databases

HSSP

P08200; 1PB1. [HSSP ENTRY / PDB]

ModBase

P80046.

Enzyme and pathway databases

BioCyc

SSP1148:SLR1289-MON; -.

Family and domain databases

InterPro

IPR004439; IsoCit_DHase_NADP_prok.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF1; NADP_IDH_prok; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00183; prok_nadp_idh; 1.

PROSITE

PS00470; IDH_IMDH; 1.

BLOCKS

P80046.

Genome annotation databases

GeneID

953454; -.

GenomeReviews

BA000022_GR; slr1289.

KEGG

syn:slr1289; -.

Phylogenomic databases

HOGENOM

P80046; -.

Genome annotation databases

CMR

P80046; slr1289.

Other

ProtoNet

P80046.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 475`	`475`	`Isocitrate dehydrogenase [NADP].`	`PRO_0000083570`
`NP_BIND`	`394 400`	`7`	`NADP (By similarity).`
`METAL`	`362 362`		`Magnesium or manganese (By similarity).`
`BINDING`	`104 104`		`NADP (By similarity).`
`BINDING`	`113 113`		`Substrate (By similarity).`
`BINDING`	`115 115`		`Substrate (By similarity).`
`BINDING`	`119 119`		`Substrate (By similarity).`
`BINDING`	`129 129`		`Substrate (By similarity).`
`BINDING`	`153 153`		`Substrate (By similarity).`
`BINDING`	`407 407`		`NADP; via amide nitrogen and carbonyl oxygen (By similarity).`
`BINDING`	`446 446`		`NADP (By similarity).`
`BINDING`	`450 450`		`NADP (By similarity).`
`SITE`	`160 160`	`1`	`Critical for catalysis (By similarity).`
`SITE`	`237 237`	`1`	`Critical for catalysis (By similarity).`
`MOD_RES`	`113 113`		`Phosphoserine (By similarity).`
`CONFLICT`	`60 62`		`REE -> ERK (in Ref. 2; BAA16835).`
`CONFLICT`	`83 84`		`IF -> YL (in Ref. 2; BAA16835).`
`CONFLICT`	`223 223`		`K -> L (in Ref. 2; BAA16835).`
`CONFLICT`	`247 247`		`P -> A (in Ref. 2; BAA16835).`
`CONFLICT`	`273 273`		`C -> L (in Ref. 2; BAA16835).`

Sequence information

Length: 475 AA [This is the length of the unprocessed precursor]

Molecular weight: 52275 Da [This is the MW of the unprocessed precursor]

CRC64: 6F3254A8BBA75F92 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MYEKLQPPSV GSKITFVAGK PVVPNDPIIP YIRGDGTGVD IWPATELVIN AAIAKAYGGR 

        70         80         90        100        110        120 
EEINWFKVYA GDEACELYGT YQIFPEDTLT AIKEYGVAIK GPLTTPVGGG IRSLNVALRQ 

       130        140        150        160        170        180 
IFDLYTCVRP CRYYPGTPSP HKTPEKLDII VYRENTEDIY LGIEWAEGTE GAKKLIAYLN 

       190        200        210        220        230        240 
DELIPTTPAL GKKQIRLDSG IGIKPISKTG SQRLVRRAIL HAKRLPKAKQ MVTLVHKGNI 

       250        260        270        280        290        300 
MKFTEGPFRD WGYELATTEF RAECVTERES WICGNKESNP DLTIEANAHM IDPGYDTLTE 

       310        320        330        340        350        360 
EKQAVIKQEV EQVLNSIWES HGNGQWKEKV MVNDRIADSI FQQIQTRPDE YSILATMNLN 

       370        380        390        400        410        420 
GDYLSDAAAA VVGGLGMGPG ANIGDSAAIF EATHGTAPKH AGLDRINPGS VILSGVMMLE 

       430        440        450        460        470 
FMGWQEAADL IKKGIGAAIA NREVTYDLAR LMEPKVDKPL KCSEFAQAIV SHFDD

P80046 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools