[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; Kawamukai M.; Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=K12 / CS520; PubMed=9098055 [NCBI, ExPASy, EBI, Israel, Japan] Ferrandez A., Garcia J.L., Diaz E.; "Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12."; J. Bacteriol. 179:2573-2581(1997).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / MG1655 / ATCC 47076; Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.; "Sequence of minutes 4-25 of Escherichia coli."; Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / MG1655 / ATCC 47076; DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan] Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.; "The complete genome sequence of Escherichia coli K-12."; Science 277:1453-1474(1997).
[5]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan] Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."; Mol. Syst. Biol. 2:E1-E5(2006).
[6]	INTERACTION WITH MHPF, AND FUNCTION. DOI=10.1016/j.bbrc.2006.06.009; PubMed=16782065 [NCBI, ExPASy, EBI, Israel, Japan] Lee S.J., Ko J.H., Kang H.Y., Lee Y.; "Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli."; Biochem. Biophys. Res. Commun. 346:1009-1015(2006).

Comments

FUNCTION: Catalyzes the terminal reaction in meta-cleavage pathways, that is, the transformation of acetaldehyde into acetyl coenzyme A. Functions as a chaperone protein for folding of mhpE.
CATALYTIC ACTIVITY: Acetaldehyde + CoA + NAD⁺ = acetyl-CoA + NADH.
PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid degradation .
SUBUNIT: Interacts with mhpE.
SIMILARITY: Belongs to the acetaldehyde dehydrogenase family. MhpF subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D86239; BAA13056.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y09555; CAA70751.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U73857; AAB18075.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC73454.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE76133.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

G64762; G64762.

RefSeq

AP_001003.1; -.
NP_414885.1; -.

3D structure databases

SMR

P77580; 3-308.

ModBase

P77580.

Protein-protein interaction databases

IntAct

P77580; -.

Enzyme and pathway databases

BioCyc

EcoCyc:MHPF-MON; -.
MetaCyc:MHPF-MON; -.

Organism-specific databases

EchoBASE

EB3390; -.

EcoGene

EG13625; mhpF.

Ontologies

GO:0008774;	Molecular function: acetaldehyde dehydrogenase (acetylating) activity (inferred from electronic annotation from HAMAP).
GO:0019439;	Biological process: aromatic compound catabolic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01657; -; 1.
PBIL [Tree]

InterPro

IPR003361; Acetylald_dehydrogenase.
IPR015426; Acetylald_DHase_C.
IPR000534; Semialdehyde_DHase_NAD-bd.
Graphical view of domain structure.

PANTHER

PTHR21123; Acetylald_dh; 1.

Pfam

PF09290; AcetDehyd-dimer; 1.
PF01118; Semialdhyde_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF015689; Actaldh_dh_actl; 1.

TIGRFAMs

TIGR03215; ac_ald_DH_ac; 1.

BLOCKS

P77580.

Genome annotation databases

GeneID

945008; -.

GenomeReviews

U00096_GR; b0351.
AP009048_GR; JW0342.

KEGG

ecj:JW0342; -.
eco:b0351; -.

Phylogenomic databases

HOGENOM

P77580; -.

Genome annotation databases

CMR

P77580; b0351.

Other

ProtoNet

P77580.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Aromatic hydrocarbons catabolism; Complete proteome; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 316`	`316`		`Acetaldehyde dehydrogenase.`	`PRO_0000096471`

Sequence information

Length: 316 AA [This is the length of the unprocessed precursor]

Molecular weight: 33442 Da [This is the MW of the unprocessed precursor]

CRC64: A6918BDA5EF4876B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSKRKVAIIG SGNIGTDLMI KILRHGQHLE MAVMVGIDPQ SDGLARARRM GVATTHEGVI 

        70         80         90        100        110        120 
GLMNMPEFAD IDIVFDATSA GAHVKNDAAL REAKPDIRLI DLTPAAIGPY CVPVVNLEAN 

       130        140        150        160        170        180 
VDQLNVNMVT CGGQATIPMV AAVSRVARVH YAEIIASIAS KSAGPGTRAN IDEFTETTSR 

       190        200        210        220        230        240 
AIEVVGGAAK GKAIIVLNPA EPPLMMRDTV YVLSDEASQD DIEASINEMA EAVQAYVPGY 

       250        260        270        280        290        300 
RLKQRVQFEV IPQDKPVNLP GVGQFSGLKT AVWLEVEGAA HYLPAYAGNL DIMTSSALAT 

       310 
AEKMAQSLAR KAGEAA

P77580 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools