[1]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=1770951 [NCBI, ExPASy, EBI, Israel, Japan] Olsen S.R., Uhler M.D.; "Inhibition of protein kinase-A by overexpression of the cloned human protein kinase inhibitor."; Mol. Endocrinol. 5:1246-1256(1991).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Knoell R.; Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.; "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."; Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Skeletal muscle; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.
MISCELLANEOUS: The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.
SIMILARITY: Belongs to the PKI family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S76965; AAB21141.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF234641; AAF40206.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CR457052; CAG33333.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC022265; AAH22265.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00220907; -.

PIR

A40468; A40468.

RefSeq

NP_006814.1; -.
NP_862822.1; -.

UniGene

Hs.433700

3D structure databases

PDB

1CMK; X-ray; 2.90 A; I=6-26.	[ExPASy / RCSB / EBI]
1JLU; X-ray; 2.25 A; S=6-25.	[ExPASy / RCSB / EBI]
1Q8T; X-ray; 2.00 A; B=6-25.	[ExPASy / RCSB / EBI]
1VEB; X-ray; 2.89 A; B=6-25.	[ExPASy / RCSB / EBI]
1XH4; X-ray; 2.45 A; B=6-25.	[ExPASy / RCSB / EBI]
1XH5; X-ray; 2.05 A; B=6-25.	[ExPASy / RCSB / EBI]
1XH6; X-ray; 1.90 A; B=6-25.	[ExPASy / RCSB / EBI]
1XH7; X-ray; 2.47 A; B=6-25.	[ExPASy / RCSB / EBI]
1XH8; X-ray; 1.60 A; B=6-25.	[ExPASy / RCSB / EBI]
1XH9; X-ray; 1.64 A; B=6-25.	[ExPASy / RCSB / EBI]
1XHA; X-ray; 2.46 A; B=6-25.	[ExPASy / RCSB / EBI]
1YDR; X-ray; 2.20 A; I=6-25.	[ExPASy / RCSB / EBI]
2C1A; X-ray; 1.95 A; I=6-25.	[ExPASy / RCSB / EBI]
2C1B; X-ray; 2.00 A; I=6-25.	[ExPASy / RCSB / EBI]
2F7E; X-ray; 2.00 A; I=6-25.	[ExPASy / RCSB / EBI]
2GNI; X-ray; 2.27 A; I=6-25.	[ExPASy / RCSB / EBI]
2JDS; X-ray; 2.00 A; I=6-25.	[ExPASy / RCSB / EBI]
2JDT; X-ray; 2.15 A; I=6-25.	[ExPASy / RCSB / EBI]
2JDV; X-ray; 2.08 A; I=6-25.	[ExPASy / RCSB / EBI]
2UVX; X-ray; 2.00 A; I=6-25.	[ExPASy / RCSB / EBI]
2UVY; X-ray; 1.95 A; I=6-25.	[ExPASy / RCSB / EBI]
2UVZ; X-ray; 1.94 A; I=6-25.	[ExPASy / RCSB / EBI]
2UW0; X-ray; 2.00 A; I=6-25.	[ExPASy / RCSB / EBI]
2UW3; X-ray; 2.19 A; I=6-25.	[ExPASy / RCSB / EBI]
2UW4; X-ray; 2.00 A; I=6-25.	[ExPASy / RCSB / EBI]
2UW5; X-ray; 2.14 A; I=6-25.	[ExPASy / RCSB / EBI]
2UW6; X-ray; 2.23 A; I=6-25.	[ExPASy / RCSB / EBI]
2UW7; X-ray; 2.10 A; I=6-25.	[ExPASy / RCSB / EBI]
2UW8; X-ray; 2.00 A; I=6-25.	[ExPASy / RCSB / EBI]
2VNW; X-ray; 2.09 A; I=6-25.	[ExPASy / RCSB / EBI]
2VNY; X-ray; 1.96 A; I=6-25.	[ExPASy / RCSB / EBI]
2VO0; X-ray; 1.94 A; I=6-25.	[ExPASy / RCSB / EBI]
2VO3; X-ray; 1.98 A; I=6-25.	[ExPASy / RCSB / EBI]
2VO6; X-ray; 1.97 A; I=6-25.	[ExPASy / RCSB / EBI]
2VO7; X-ray; 1.98 A; I=6-25.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1CMK; -.
1JLU; -.
1Q8T; -.
1VEB; -.
1XH4; -.
1XH5; -.
1XH6; -.
1XH7; -.
1XH8; -.
1XH9; -.
1XHA; -.
1YDR; -.
2C1A; -.
2C1B; -.
2F7E; -.
2GNI; -.
2JDS; -.
2JDT; -.
2JDV; -.
2UVX; -.
2UVY; -.
2UVZ; -.
2UW0; -.
2UW3; -.
2UW4; -.
2UW5; -.
2UW6; -.
2UW7; -.
2UW8; -.
2VNW; -.
2VNY; -.
2VO0; -.
2VO3; -.
2VO6; -.
2VO7; -.

ModBase

P61925.

PTM databases

PhosphoSite

P61925; -.

Organism-specific databases

GC08P079590; -.

HIX0007603; -.

HGNC:9017; PKIA.

606059; gene. [NCBI / EBI]

PharmGKB

PA33349; -.

Gene expression databases

P61925; -.

P61925; -.

HS_PKIA; -.

ENSG00000171033; Homo sapiens.

Ontologies

GO:0004862;	Molecular function: cAMP-dependent protein kinase inhibitor activity (traceable author statement from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR004171; cAMP_dep_PKI.
Graphical view of domain structure.

Pfam

PF02827; PKI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF001667; PKI; 1.

ProDom

PD010366; cAMP_dep_PKI; 1.
[Domain structure / List of seq. sharing at least 1 domain]

Genome annotation databases

Ensembl

ENSG00000171033; Homo sapiens. [Contig view]

GeneID

5569; -.

KEGG

hsa:5569; -.

Phylogenomic databases

HOGENOM

P61925; -.

HOVERGEN

P61925; -.

OMA

P61925; DVESTYA.

Other

21582; -.

PKIA; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Protein kinase inhibitor.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 76`	`75`	`cAMP-dependent protein kinase inhibitor alpha.`	`PRO_0000154532`
`SITE`	`16 16`	`1`	`Important for inhibition (By similarity).`
`SITE`	`19 19`	`1`	`Important for inhibition (By similarity).`
`SITE`	`20 20`	`1`	`Important for inhibition (By similarity).`
`MOD_RES`	`2 2`		`Blocked amino end (Thr) (By similarity).`
`HELIX`	`7 12`	`6`

Sequence information

Length: 76 AA [This is the length of the unprocessed precursor]

Molecular weight: 7989 Da [This is the MW of the unprocessed precursor]

CRC64: BDCE072810435951 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT EGEEDAQRSS 

        70 
TEQSGEAQGE AAKSES

P61925 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools