NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Tokyo 1998;
DOI=10.1038/35024074; PubMed=10993077 [NCBI, ExPASy, EBI, Israel, Japan]
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS.";
Nature 407:81-86(2000).

Comments

FUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).
CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO₂.
COFACTOR: Thiamine pyrophosphate (By similarity).
SUBUNIT: Homodimer (By similarity).

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000003; BAB12922.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_240036.1; -.

3D structure databases

HSSP

P06958; 1L8A. [HSSP ENTRY / PDB]

SMR

P57301; 58-887.

ModBase

P57301.

Enzyme and pathway databases

BioCyc

BSP107806:BU205-MON; -.

Family and domain databases

InterPro

IPR004660; 2-oxoA_DHase_E1.
IPR005474; Transketo_N.
IPR015941; Transketolase_C-like.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.920; Transketo_C_like; 1.

Pfam

PF00456; Transketolase_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000156; Pyruvate_dh_E1; 1.

TIGRFAMs

TIGR00759; aceE; 1.

BLOCKS

P57301.

Genome annotation databases

GeneID

1109648; -.

GenomeReviews

BA000003_GR; BU205.

KEGG

buc:BU205; -.

Phylogenomic databases

HOGENOM

P57301; -.

Genome annotation databases

CMR

P57301; BU205.

Other

ProtoNet

P57301.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Glycolysis; Oxidoreductase; Pyruvate; Thiamine pyrophosphate.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 887`	`887`		`Pyruvate dehydrogenase E1 component.`	`PRO_0000162240`

Sequence information

Length: 887 AA [This is the length of the unprocessed precursor]

Molecular weight: 101396 Da [This is the MW of the unprocessed precursor]

CRC64: B6AB82C012826105 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSENLYNDVD PIETRDWVQA IESVIRREGH KRAHFLIEQV LKTAKINRKE FFRSSFTSDY 

        70         80         90        100        110        120 
INTISREDEY EYPGNLILEK RIRSAIRWNA IMMVLRASKK NLELGGHLSS FQSSATIYEV 

       130        140        150        160        170        180 
CFNHFFQAKN HKDGGDLVYF QGHISPGIYA RSFLEGRLSE EQIDNFRQEV DGIGLSSYPH 

       190        200        210        220        230        240 
PKLMPNFWQF PTVSMGLGPL CAIYQAKFLK YLHNRELKNT SKQIVYAFLG DGEMDEPESK 

       250        260        270        280        290        300 
GAISIAVREK LDNLIFIINC NLQRLDGPVV GNGKIVNELE SFFYGAGWKV IKVIWGSRWD 

       310        320        330        340        350        360 
CLLKKDTSGK LIQLMNETVD GDYQTFKSKD GAYVRKYFFG KYKETYDLVK DMTDEEIWKL 

       370        380        390        400        410        420 
NRGGHDPKKM FNALKKAKET KYKPTVILAH TVKGYGMGVI AEGKNIAHQI KKININGIIH 

       430        440        450        460        470        480 
IRDRFNIPVS NDEINKLPYV TFKKNSEEYC YIHSQRKKLG GYIPFRLSSF TGKLILPKLI 

       490        500        510        520        530        540 
DFQSLLEEQK KDISTTVAFI RVLNIILKNN SIKHLIVPII ADEARTFGME GLFRKIGIYS 

       550        560        570        580        590        600 
SSGQKYTPQD REQLAYYKEE KKGQILQEGI NELGAASSWL AAATSYSTND FPMILFYIYY 

       610        620        630        640        650        660 
SIFGFQRIGD LFWAAGDQQA RGFLIGGTSG RTTLNGEGLQ HEDGHSHIQS LTIPNCISYD 

       670        680        690        700        710        720 
PAFAYEVAVI IQDGLRRMYG PSQENIYYYI TTINENYYMP AMPIGVEEGI CKGIYKLKTL 

       730        740        750        760        770        780 
HGTTSKVQLI GSGAILRSVC EAAEILLKDY SITTDIYSVT SFTELARNGE DCERWNMLHP 

       790        800        810        820        830        840 
NEKNKIAYVK QIMNKNPTVA ATDYMKLFAE QIRHYIPSQE YHVLGTDGFG RSDSRDKLRD 

       850        860        870        880 
HFEVNAYYIV IAALNLLANI NDIKKKVVED AIMKFNIDAN KINPRLS

P57301 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools