ID   NUOB_BUCAI              Reviewed;         224 AA.
AC   P57253;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   25-NOV-2008, entry version 43.
DE   RecName: Full=NADH-quinone oxidoreductase subunit B;
DE            EC=1.6.99.5;
DE   AltName: Full=NADH dehydrogenase I subunit B;
DE   AltName: Full=NDH-1 subunit B;
GN   Name=nuoB; OrderedLocusNames=BU155;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum
OS   symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=118099;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tokyo 1998;
RX   MEDLINE=20445173; PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids
RT   Buchnera sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-
CC       sulfur (Fe-S) centers, to quinones in the respiratory chain.
CC       Couples the redox reaction to proton translocation (for every two
CC       electrons transferred, four hydrogen ions are translocated across
CC       the cytoplasmic membrane), and thus conserves the redox energy in
CC       a proton gradient (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + quinone = NAD(+) + quinol.
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster (Potential).
CC   -!- SUBUNIT: Composed of 13 different subunits (By similarity).
CC   -!- SIMILARITY: Belongs to the complex I 20 kDa subunit family.
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DR   EMBL; BA000003; BAB12873.1; -; Genomic_DNA.
DR   RefSeq; NP_239987.1; -.
DR   GeneID; 1109599; -.
DR   GenomeReviews; BA000003_GR; BU155.
DR   KEGG; buc:BU155; -.
DR   HOGENOM; P57253; -.
DR   BioCyc; BSP107806:BU155-MON; -.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to u...; IEA:InterPro.
DR   InterPro; IPR006138; NADH_DHase_20kDa_su.
DR   InterPro; IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
DR   InterPro; IPR006137; OxRdtase_q6.
DR   PANTHER; PTHR11995:SF2; NADH_DH_20kDa; 1.
DR   PANTHER; PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.
DR   Pfam; PF01058; Oxidored_q6; 1.
DR   TIGRFAMs; TIGR01957; nuoB_fam; 1.
DR   PROSITE; PS01150; COMPLEX1_20K; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding; NAD;
KW   Oxidoreductase; Quinone.
FT   CHAIN         1    224       NADH-quinone oxidoreductase subunit B.
FT                                /FTId=PRO_0000118769.
FT   METAL        67     67       Iron-sulfur (4Fe-4S) (Potential).
FT   METAL        68     68       Iron-sulfur (4Fe-4S) (Potential).
FT   METAL       133    133       Iron-sulfur (4Fe-4S) (Potential).
FT   METAL       162    162       Iron-sulfur (4Fe-4S) (Potential).
SQ   SEQUENCE   224 AA;  25597 MW;  BB450B102C87CD7B CRC64;
     MNYTLTKADS DNNNKKYPKQ TIESVSDPLE EYLKKNIFMG KITQLLHKLV NWGRKNSLWP
     YNFGLSCCYV EMVSAFTSVH DVARFGSEVL RASPRQADVM VIAGTPFIKM APVIQRLYDQ
     MLEPKWVISM GACANSGGMY DIYSVVQGVD KFLPVDIYIP GCPPRPEAYM QALILLQKLI
     NEERRPLSWV IGEQGVYHKK MPSERVQKRS KRINIINLST SEKI
//