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UniProtKB/Swiss-Prot entry P56897


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NUOB2_RHIME
Primary accession number P56897
Secondary accession numbers None
Integrated into Swiss-Prot on May 30, 2000
Sequence was last modified on October 24, 2001 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 50)
Name and origin of the protein
Protein name NADH-quinone oxidoreductase subunit B 2
Synonyms EC 1.6.99.5
NADH dehydrogenase I subunit B 2
NDH-1 subunit B 2
Gene name
Name: nuoB2
OrderedLocusNames: RA0833
ORFNames: SMa1532
From
Rhizobium meliloti (Sinorhizobium meliloti) [TaxID: 382] [HAMAP proteome]
Encoded on Plasmid pSymA (megaplasmid 1).
Taxonomy Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=41;
Putnoky P., Jady B., Chellapilla K.P., Barta F., Kiss E.;
"Rhizobium meliloti carries two sets of nuo genes.";
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=1021;
DOI=10.1073/pnas.161294798; PubMed=11481432 [NCBI, ExPASy, EBI, Israel, Japan]
Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R.;
"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid.";
Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
Comments
  • FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).
  • CATALYTIC ACTIVITY: NADH + quinone = NAD+ + quinol.
  • COFACTOR: Binds 1 4Fe-4S cluster (Potential).
  • SIMILARITY: Belongs to the complex I 20 kDa subunit family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AJ245399; CAB51630.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE006469; AAK65491.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A95366; A95366.
RefSeq NP_436079.1; -.
3D structure databases
ModBase P56897.
Enzyme and pathway databases
BioCyc SMEL266834:SMA1532-MON; -.
Family and domain databases
InterPro IPR006138; NADH_DHase_20kDa_su.
IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.
PANTHER PTHR11995:SF2; NADH_DH_20kDa; 1.
PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.
Pfam PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01957; nuoB_fam; 1.
BLOCKS P56897.
Genome annotation databases
GeneID 1235869; -.
GenomeReviews AE006469_GR; RA0833.
KEGG sme:SMa1532; -.
NMPDR fig|266834.1.peg.833; -.
Phylogenomic databases
HOGENOM P56897; -.
Genome annotation databases
CMR P56897; RA0833.
Other
ProtoNet P56897.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Plasmid; Quinone; Ubiquinone.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   167  167     NADH-quinone oxidoreductase subunit B 2. PRO_0000118776
METAL   38    38        Iron-sulfur (4Fe-4S) (Potential). 
METAL   39    39        Iron-sulfur (4Fe-4S) (Potential). 
METAL   103   103        Iron-sulfur (4Fe-4S) (Potential). 
METAL   132   132        Iron-sulfur (4Fe-4S) (Potential). 
CONFLICT   167   167        S -> L (in Ref. 1; CAB51630). 
Sequence information
Length: 167 AA [This is the length of the unprocessed precursor] Molecular weight: 18247 Da [This is the MW of the unprocessed precursor] CRC64: 04D82AAED624D8CF [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAGVNDAIRD SVLFTTADSI ISWSRRSALW PETFGIACCA IEMISAGCAR YDLDRFGVVF 

        70         80         90        100        110        120 
RPSPRQSDVM IIAGTVTRKF APVVRRLYDQ MPEPRWVIAM GTCAISGGVY NTYAVVQGSE 

       130        140        150        160 
TFVPVDVHVP GCPPRPEALM HGFLLLQEKI KKSRALTGTP LGRVIAS 

P56897 in FASTA format

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