NUCLEOTIDE SEQUENCE.
DOI=10.1006/bbrc.1997.6750; PubMed=9196051 [NCBI, ExPASy, EBI, Israel, Japan]
Akiyama H., Shigeno C., Hiraki Y., Shukunami C., Kohno H., Akagi M., Konishi J., Nakamura T.;
"Cloning of a mouse Smoothened cDNA and expression patterns of hedgehog signalling molecules during chondrogenesis and cartilage differentiation in clonal mouse EC cells, ATDC5.";
Biochem. Biophys. Res. Commun. 235:142-147(1997).

Comments

FUNCTION: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the G-protein coupled receptor Fz/Smo family [view classification].
SIMILARITY: Contains 1 FZ (frizzled) domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

IPI00341032; -.

JC5539; JC5539.

3D structure databases

ModBase

P56726.

Protein family/group databases

GPCRDB

P56726; SMO_MOUSE.

Organism-specific databases

MGI

MGI:108075; Smo.

Gene expression databases

P56726; -.

P56726; -.

MM_SMO; -.

ENSMUSG00000001761; Mus musculus.

Ontologies

GO:0005929;	Cellular component: cilium (inferred from direct assay from MGI).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004930;	Molecular function: G-protein coupled receptor activity (inferred from electronic annotation from UniProtKB-KW).
GO:0004926;	Molecular function: non-G-protein coupled 7TM receptor activity (inferred from electronic annotation from InterPro).
GO:0001708;	Biological process: cell fate specification (inferred from mutant phenotype from MGI).
GO:0021953;	Biological process: central nervous system neuron differentiation (inferred from mutant phenotype from MGI).
GO:0021696;	Biological process: cerebellar cortex morphogenesis (inferred from mutant phenotype from MGI).
GO:0007368;	Biological process: determination of left/right symmetry (inferred from mutant phenotype from MGI).
GO:0048589;	Biological process: developmental growth (inferred from mutant phenotype from MGI).
GO:0021904;	Biological process: dorsal/ventral neural tube patterning (inferred from mutant phenotype from MGI).
GO:0007186;	Biological process: G-protein coupled receptor protein signaling pathway (inferred from electronic annotation from UniProtKB-KW).
GO:0003007;	Biological process: heart morphogenesis (inferred from genetic interaction from MGI).
GO:0035264;	Biological process: multicellular organism growth (inferred from genetic interaction from MGI).
GO:0043066;	Biological process: negative regulation of apoptosis (inferred from mutant phenotype from MGI).
GO:0043392;	Biological process: negative regulation of DNA binding (inferred from genetic interaction from MGI).
GO:0001755;	Biological process: neural crest cell migration (inferred from genetic interaction from MGI).
GO:0042475;	Biological process: odontogenesis of dentine-containing tooth (inferred from mutant phenotype from MGI).
GO:0001649;	Biological process: osteoblast differentiation (inferred from mutant phenotype from MGI).
GO:0002052;	Biological process: positive regulation of neuroblast proliferation (inferred from genetic interaction from MGI).
GO:0042307;	Biological process: positive regulation of protein import into nucleus (inferred from genetic interaction from MGI).
GO:0010468;	Biological process: regulation of gene expression (inferred from genetic interaction from MGI).
GO:0021938;	Biological process: smoothened signaling pathway involved in regulation of granule cell precursor cell proliferation (inferred from mutant phenotype from MGI).
GO:0021910;	Biological process: smoothened signaling pathway involved in ventral spinal cord patterning (inferred from mutant phenotype from MGI).
GO:0001570;	Biological process: vasculogenesis (inferred from mutant phenotype from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR000539; Frizzled.
IPR000024; Frizzled_Cys-rich.
IPR015526; Frizzled_related.
IPR017981; GPCR_2-like.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.2000.10; Frizzled_Cys-rich; 1.

PANTHER

PTHR11309; Fz_related; 1.

Pfam

PF01534; Frizzled; 1.
Pfam graphical view of domain structure.

PRINTS

PR00489; FRIZZLED.

PROSITE

PS50038; FZ; 1.
PS50261; G_PROTEIN_RECEP_F2_4; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSMUSG00000001761; Mus musculus. [Contig view]

Phylogenomic databases

HOGENOM

P56726; -.

HOVERGEN

P56726; -.

Other

Smo; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Developmental protein; Disulfide bond; G-protein coupled receptor; Glycoprotein; Membrane; Receptor; Signal; Transducer; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 32`	`32`	`Potential.`
`CHAIN`	`33 793`	`761`	`Smoothened homolog.`	`PRO_0000013016`
`TOPO_DOM`	`33 237`	`205`	`Extracellular (Potential).`
`TRANSMEM`	`238 258`	`21`	`1 (Potential).`
`TOPO_DOM`	`259 265`	`7`	`Cytoplasmic (Potential).`
`TRANSMEM`	`266 286`	`21`	`2 (Potential).`
`TOPO_DOM`	`287 318`	`32`	`Extracellular (Potential).`
`TRANSMEM`	`319 339`	`21`	`3 (Potential).`
`TOPO_DOM`	`340 362`	`23`	`Cytoplasmic (Potential).`
`TRANSMEM`	`363 383`	`21`	`4 (Potential).`
`TOPO_DOM`	`384 406`	`23`	`Extracellular (Potential).`
`TRANSMEM`	`407 427`	`21`	`5 (Potential).`
`TOPO_DOM`	`428 455`	`28`	`Cytoplasmic (Potential).`
`TRANSMEM`	`456 476`	`21`	`6 (Potential).`
`TOPO_DOM`	`477 528`	`52`	`Extracellular (Potential).`
`TRANSMEM`	`529 549`	`21`	`7 (Potential).`
`TOPO_DOM`	`550 793`	`244`	`Cytoplasmic (Potential).`
`DOMAIN`	`69 185`	`117`	`FZ.`
`CARBOHYD`	`38 38`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`192 192`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`497 497`		`N-linked (GlcNAc...) (Potential).`
`DISULFID`	`74 138`		`By similarity.`
`DISULFID`	`82 131`		`By similarity.`
`DISULFID`	`122 158`		`By similarity.`
`DISULFID`	`151 173`		`By similarity.`

Sequence information

Length: 793 AA [This is the length of the unprocessed precursor]

Molecular weight: 87300 Da [This is the MW of the unprocessed precursor]

CRC64: 10B2767876171167 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAGRPVRGP ELAPRRLLQL LLLVLLGGPG RGAALSGNVT GPGPHSASGS SRRNVPVTSP 

        70         80         90        100        110        120 
PPPLLSHCGR AAHCEPLRYN VCLGSALPYG ATTTLLAGDS DSQEEAHGKL VLWSGLRNAP 

       130        140        150        160        170        180 
RCWAVIQPLL CAVYMPKCEN DRVELPSRTL CQATRGPCAI VERERGWPDF LRCTPDHFPE 

       190        200        210        220        230        240 
GCPNEVQNIK FNSSGQCEAP LVRTDNPKSW YEDVEGCGIQ CQNPLFTEAE HQDMHSYIAA 

       250        260        270        280        290        300 
FGAVTGLCTL FTLATFVADW RNSNRLPAVI LFYVNACFFV GSIGWLAQFM DGARREIVCR 

       310        320        330        340        350        360 
ADGTMRFGEP TSSETLSCVI IFVIVYYALM AGVVWFVVLT YAWHTSFKAL GTTYQPLSGK 

       370        380        390        400        410        420 
TSYFHLLTWS LPFVLTVAIL AVAQVDGDSV SGICFVGYKN YRYRAGFVLA PIGLVLIVGG 

       430        440        450        460        470        480 
YFLIRGVMTL FSIKSNHPGL LSEKAASKIN ETMLRLGIFG FLAFGFVLIT FSCHFYDFFN 

       490        500        510        520        530        540 
QAEWERSFRD YVLCQANVTI GLPTKKPIPD CEIKNRPSLL VEKINLFAMF GTGIAMSTWV 

       550        560        570        580        590        600 
WTKATLLIWR RTWCRLTGHS DDEPKRIKKS KMIAKAFSKR RELLQNPGQE LSFSMHTVSH 

       610        620        630        640        650        660 
DGPVAGLAFD LNEPSADVSS AWAQHVTKMV ARRGAILPQD VSVTPVATPV PPEEQANMWL 

       670        680        690        700        710        720 
VEAEISPELE KRLGRKKKRR KRKKEVCPLG PAPELHHSAP VPATSAVPRL PQLPRQKCLV 

       730        740        750        760        770        780 
AANAWGTGES CRQGAWTLVS NPFCPEPSPH QDPFLPGASA PRVWAQGRLQ GLGSIHSRTN 

       790 
LMEAEILDAD SDF

P56726 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools