[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1007/BF00020228; PubMed=7727752 [NCBI, ExPASy, EBI, Israel, Japan] Navarro F., Chavez S., Candau P., Florencio F.J.; "Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes."; Plant Mol. Biol. 27:753-767(1995).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Terauchi K., Ikeuchi M., Ohmori M.; Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1093/dnares/3.3.109; PubMed=8905231 [NCBI, ExPASy, EBI, Israel, Japan] Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.; "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."; DNA Res. 3:109-136(1996).

Comments

CATALYTIC ACTIVITY: 2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H⁺.
COFACTOR: Binds 1 3Fe-4S cluster.
COFACTOR: FAD.
COFACTOR: FMN.
PATHWAY: Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1 .
PATHWAY: Energy metabolism; nitrogen metabolism .
INTERACTION:
P52231:trxA; NbExp=1; IntAct=EBI-862080, EBI-862916;
SIMILARITY: Belongs to the glutamate synthase family.
SIMILARITY: Contains 1 glutamine amidotransferase type-2 domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X92480; CAA63218.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D78371; BAA11379.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000022; BAA18693.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S76781; S76781.

RefSeq

NP_442881.1; -.

3D structure databases

PDB

1LLW; X-ray; 2.70 A; A=37-1556.	[ExPASy / RCSB / EBI]
1LLZ; X-ray; 3.00 A; A=37-1556.	[ExPASy / RCSB / EBI]
1LM1; X-ray; 2.80 A; A=37-1556.	[ExPASy / RCSB / EBI]
1OFD; X-ray; 2.00 A; A/B=37-1556.	[ExPASy / RCSB / EBI]
1OFE; X-ray; 2.45 A; A/B=37-1556.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1LLW; -.
1LLZ; -.
1LM1; -.
1OFD; -.
1OFE; -.

ModBase

P55038.

Protein-protein interaction databases

IntAct

P55038; 4.

Enzyme and pathway databases

BioCyc

SSP1148:SLL1499-MON; -.

Ontologies

GO:0051538;	Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0016041;	Molecular function: glutamate synthase (ferredoxin) activity (inferred from electronic annotation from EC).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006537;	Biological process: glutamate biosynthetic process (inferred from electronic annotation from InterPro).
GO:0006541;	Biological process: glutamine metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR013785; Aldolase_TIM.
IPR000583; GATase_2.
IPR002932; Glu_synth_centr_C.
IPR006982; Glu_synth_centr_N.
IPR002489; Glu_synthase_C.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 2.
G3DSA:2.160.20.60; Glu_synthase_C; 1.

Pfam

PF00310; GATase_2; 1.
PF04898; Glu_syn_central; 1.
PF01645; Glu_synthase; 1.
PF01493; GXGXG; 1.
Pfam graphical view of domain structure.

PROSITE

PS51278; GATASE_TYPE_2; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

GeneID

952019; -.

GenomeReviews

BA000022_GR; sll1499.

KEGG

syn:sll1499; -.

NMPDR

fig|1148.1.peg.2982; -.

Phylogenomic databases

HOGENOM

P55038; -.

Genome annotation databases

CMR

P55038; sll1499.

Other

ProtoNet

P55038.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; 3Fe-4S; Amino-acid biosynthesis; Complete proteome; FAD; Flavoprotein; FMN; Glutamate biosynthesis; Glutamine amidotransferase; Iron; Iron-sulfur; Metal-binding; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1556`	`1556`	`Ferredoxin-dependent glutamate synthase 2.`	`PRO_0000170794`
`DOMAIN`	`37 431`	`395`	`Glutamine amidotransferase type-2.`
`ACT_SITE`	`37 37`		`For GATase activity (By similarity).`
`METAL`	`1173 1173`		`Iron-sulfur (3Fe-4S) (By similarity).`
`METAL`	`1179 1179`		`Iron-sulfur (3Fe-4S) (By similarity).`
`METAL`	`1184 1184`		`Iron-sulfur (3Fe-4S) (By similarity).`
`CONFLICT`	`491 491`		`E -> Q (in Ref. 1; CAA63218).`
`CONFLICT`	`570 572`		`ESA -> NPR (in Ref. 1; CAA63218).`
`CONFLICT`	`642 650`		`GAILTENQS -> RRNIGLRIKV (in Ref. 1; CAA63218).`
`CONFLICT`	`659 659`		`G -> E (in Ref. 1; CAA63218).`
`CONFLICT`	`940 941`		`GG -> PP (in Ref. 1; CAA63218).`
`CONFLICT`	`1059 1059`		`H -> L (in Ref. 1; CAA63218).`
`CONFLICT`	`1295 1295`		`E -> RK (in Ref. 1; CAA63218).`
`CONFLICT`	`1310 1310`		`V -> D (in Ref. 1; CAA63218).`
`CONFLICT`	`1323 1323`		`A -> S (in Ref. 1; CAA63218).`
`CONFLICT`	`1531 1531`		`Missing (in Ref. 1; CAA63218).`
`STRAND`	`38 43`	`6`
`STRAND`	`45 47`	`3`
`HELIX`	`52 63`	`12`
`HELIX`	`64 67`	`4`
`STRAND`	`74 78`	`5`
`STRAND`	`80 85`	`6`
`HELIX`	`88 97`	`10`
`HELIX`	`105 107`	`3`
`STRAND`	`109 115`	`7`
`HELIX`	`119 135`	`17`
`STRAND`	`139 145`	`7`
`HELIX`	`150 152`	`3`
`HELIX`	`155 160`	`6`
`STRAND`	`163 170`	`8`
`HELIX`	`177 191`	`15`
`HELIX`	`192 194`	`3`
`STRAND`	`199 215`	`17`
`HELIX`	`217 219`	`3`
`HELIX`	`220 223`	`4`
`HELIX`	`225 228`	`4`
`STRAND`	`234 241`	`8`
`STRAND`	`245 247`	`3`
`HELIX`	`251 253`	`3`
`STRAND`	`258 264`	`7`
`HELIX`	`269 279`	`11`
`HELIX`	`280 282`	`3`
`HELIX`	`290 296`	`7`
`HELIX`	`306 319`	`14`
`HELIX`	`324 331`	`8`
`HELIX`	`340 342`	`3`
`HELIX`	`346 355`	`10`
`TURN`	`356 358`	`3`
`STRAND`	`364 371`	`8`
`STRAND`	`373 380`	`8`
`STRAND`	`389 393`	`5`
`STRAND`	`398 403`	`6`
`HELIX`	`411 413`	`3`
`STRAND`	`414 419`	`6`
`STRAND`	`425 429`	`5`
`TURN`	`430 433`	`4`
`STRAND`	`434 436`	`3`
`HELIX`	`438 446`	`9`
`HELIX`	`451 458`	`8`
`STRAND`	`459 462`	`4`
`HELIX`	`476 485`	`10`
`HELIX`	`490 495`	`6`
`HELIX`	`497 503`	`7`
`TURN`	`518 520`	`3`
`HELIX`	`527 530`	`4`
`STRAND`	`531 533`	`3`
`STRAND`	`538 540`	`3`
`TURN`	`545 548`	`4`
`HELIX`	`549 551`	`3`
`STRAND`	`556 559`	`4`
`STRAND`	`564 566`	`3`
`HELIX`	`569 572`	`4`
`STRAND`	`574 578`	`5`
`HELIX`	`584 592`	`9`
`STRAND`	`593 595`	`3`
`STRAND`	`597 601`	`5`
`STRAND`	`603 605`	`3`
`STRAND`	`607 609`	`3`
`HELIX`	`612 628`	`17`
`STRAND`	`632 639`	`8`
`HELIX`	`640 642`	`3`
`STRAND`	`649 651`	`3`
`HELIX`	`654 667`	`14`
`HELIX`	`671 673`	`3`
`STRAND`	`675 679`	`5`
`HELIX`	`686 694`	`9`
`STRAND`	`698 701`	`4`
`HELIX`	`703 713`	`11`
`HELIX`	`716 722`	`7`
`HELIX`	`732 753`	`22`
`HELIX`	`759 763`	`5`
`STRAND`	`768 773`	`6`
`HELIX`	`775 781`	`7`
`HELIX`	`794 808`	`15`
`STRAND`	`820 823`	`4`
`HELIX`	`835 847`	`13`
`HELIX`	`862 871`	`10`
`HELIX`	`878 881`	`4`
`STRAND`	`882 884`	`3`
`HELIX`	`893 895`	`3`
`HELIX`	`899 903`	`5`
`TURN`	`913 915`	`3`
`HELIX`	`918 931`	`14`
`HELIX`	`944 947`	`4`
`STRAND`	`977 981`	`5`
`HELIX`	`990 995`	`6`
`STRAND`	`997 1002`	`6`
`HELIX`	`1017 1019`	`3`
`HELIX`	`1022 1028`	`7`
`HELIX`	`1048 1061`	`14`
`STRAND`	`1065 1072`	`8`
`HELIX`	`1077 1086`	`10`
`STRAND`	`1090 1095`	`6`
`STRAND`	`1102 1105`	`4`
`HELIX`	`1106 1111`	`6`
`HELIX`	`1116 1129`	`14`
`HELIX`	`1133 1135`	`3`
`STRAND`	`1137 1143`	`7`
`HELIX`	`1147 1155`	`9`
`STRAND`	`1159 1162`	`4`
`HELIX`	`1165 1170`	`6`
`HELIX`	`1179 1181`	`3`
`STRAND`	`1187 1189`	`3`
`HELIX`	`1193 1196`	`4`
`HELIX`	`1203 1224`	`22`
`HELIX`	`1229 1232`	`4`
`HELIX`	`1236 1238`	`3`
`STRAND`	`1239 1241`	`3`
`STRAND`	`1248 1251`	`4`
`HELIX`	`1256 1259`	`4`
`HELIX`	`1269 1271`	`3`
`HELIX`	`1283 1289`	`7`
`HELIX`	`1291 1299`	`9`
`STRAND`	`1302 1309`	`8`
`HELIX`	`1318 1328`	`11`
`TURN`	`1329 1331`	`3`
`STRAND`	`1336 134`