NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=European shorthair;
TISSUE=Melanoma;
PubMed=9150543 [NCBI, ExPASy, EBI, Israel, Japan]
van der Linde-Sipman J.S., de Wit M.M., van Garderen E., Molenbeek R.F., van der Velde-Zimmermann D., de Weger R.A.;
"Cutaneous malignant melanomas in 57 cats: identification of (amelanotic) signet-ring and balloon cell types and verification of their origin by immunohistochemistry, electron microscopy, and in situ hybridization.";
Vet. Pathol. 34:31-38(1997).

Comments

FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
CATALYTIC ACTIVITY: L-tyrosine + L-dopa + O₂ = L-dopa + dopaquinone + H₂O.
COFACTOR: Binds 2 copper ions per subunit.
SUBCELLULAR LOCATION: Melanosome membrane; Single-pass type I membrane protein.
SIMILARITY: Belongs to the tyrosinase family.
WEB RESOURCE: Name=Protein Spotlight; Note=Snowy stardom - Issue 49 of August 2004; URL="http://www.expasy.org/spotlight/back_issues/sptlt049.shtml";.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U40716; AAB08729.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P55033.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0033162;	Cellular component: melanosome membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005507;	Molecular function: copper ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004503;	Molecular function: monophenol monooxygenase activity (inferred from electronic annotation from EC).
GO:0006583;	Biological process: melanin biosynthetic process from tyrosine (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002227; Tyrosinase.
Graphical view of domain structure.

Pfam

PF00264; Tyrosinase; 1.
Pfam graphical view of domain structure.

PROSITE

PS00497; TYROSINASE_1; PARTIAL.
PS00498; TYROSINASE_2; PARTIAL.

Phylogenomic databases

HOVERGEN

P55033; -.

Other

ProtoNet

P55033.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Copper; Glycoprotein; Melanin biosynthesis; Membrane; Monooxygenase; Oxidoreductase; Transmembrane.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 >80`	`>80`	`Tyrosinase.`	`PRO_0000186729`
`CARBOHYD`	`59 59`		`N-linked (GlcNAc...) (Potential).`
`NON_TER`	`1 1`
`NON_TER`	`80 80`

Sequence information

Length: 80 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 8694 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 926D7C99D49B74D3 [This is a checksum on the sequence]

        10         20         30         40         50         60 
LEEYNSRQAL CDGTPEGPLL RNPGNHDKAR TPRLPSSADV EFCLSLTQYE SDSMDKAANF 

        70         80 
SFRNTLEGFA SPLTGIADAS

P55033 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools