[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Eye; DOI=10.1016/0378-1119(94)90445-6; PubMed=7821799 [NCBI, ExPASy, EBI, Israel, Japan] Inagaki H., Bessho Y., Koga A., Hori H.; "Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes."; Gene 150:319-324(1994).
[2]	NUCLEOTIDE SEQUENCE. Inagaki H., Koga A., Bessho Y., Hori H.; "The tyrosinase gene from medaka: transgenic expression rescued albino mutation."; Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.
CATALYTIC ACTIVITY: L-tyrosine + L-dopa + O₂ = L-dopa + dopaquinone + H₂O.
COFACTOR: Binds 2 copper ions per subunit.
SUBCELLULAR LOCATION: Melanosome membrane; Single-pass type I membrane protein.
SIMILARITY: Belongs to the tyrosinase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D29687; BAA06156.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D29686; BAA06155.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB010101; BAA31348.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001098272.1; -.

UniGene

Ola.86

3D structure databases

ModBase

P55025.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0033162;	Cellular component: melanosome membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005507;	Molecular function: copper ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004503;	Molecular function: monophenol monooxygenase activity (inferred from electronic annotation from EC).
GO:0006583;	Biological process: melanin biosynthetic process from tyrosine (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR008922; Di-copper_centre.
IPR002227; Tyrosinase.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.1280.10; Di-copper_centre; 1.

Pfam

PF00264; Tyrosinase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00092; TYROSINASE.

PROSITE

PS00497; TYROSINASE_1; 1.
PS00498; TYROSINASE_2; 1.

Genome annotation databases

Ensembl

ENSORLG00000010905; Oryzias latipes. [Contig view]

GeneID

100049427; -.

Phylogenomic databases

HOVERGEN

P55025; -.

Other

ProtoNet

P55025.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Copper; Glycoprotein; Melanin biosynthesis; Membrane; Metal-binding; Monooxygenase; Oxidoreductase; Signal; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 19`	`19`	`Potential.`
`CHAIN`	`20 540`	`521`	`Tyrosinase.`	`PRO_0000035883`
`TOPO_DOM`	`20 480`	`461`	`Lumenal, melanosome (Potential).`
`TRANSMEM`	`481 501`	`21`	`Potential.`
`TOPO_DOM`	`502 540`	`39`	`Cytoplasmic (Potential).`
`METAL`	`182 182`		`Copper A (By similarity).`
`METAL`	`205 205`		`Copper A (By similarity).`
`METAL`	`214 214`		`Copper A (By similarity).`
`METAL`	`366 366`		`Copper B (By similarity).`
`METAL`	`370 370`		`Copper B (By similarity).`
`METAL`	`393 393`		`Copper B (By similarity).`
`CARBOHYD`	`87 87`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`233 233`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`293 293`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`340 340`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`374 374`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 540 AA [This is the length of the unprocessed precursor]

Molecular weight: 61254 Da [This is the MW of the unprocessed precursor]

CRC64: 7ADEA02C3870CAE4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKSLFLSAVL LQFFETCWSQ FPRPCANSEG LRTKECCPVW SGDGSPCGAL SGRGFCADVS 

        70         80         90        100        110        120 
VSDEPNGPQY PHSGIDDRER WPLAFFNRTC RCAGNYGGFN CGECRFGYWG SNCAEYRESV 

       130        140        150        160        170        180 
RRNIMSMSTT EQQKFISYLN LAKNTINPDY VITTGTRAEM GENGESPMFS DINTYDLFVW 

       190        200        210        220        230        240 
IHYYVSRDTF LGGPGNVWRD IDFAHESAAF LPWHRVYLLH WEQEIRKITG DFNFTIPYWD 

       250        260        270        280        290        300 
WRDAQSCEVC TDNLMGGRNA LNPNLISPAS VFSSWKVICT QQEEYNNQEA LCNATAEGPL 

       310        320        330        340        350        360 
LRNPGNHDPN RVPRIPTTAD VEFTISLPEY ETGSMDRFAN NSFRNVLEGF ASPETGMAVQ 

       370        380        390        400        410        420 
GQSTMHNALH VFMNGSMSSV QGSANDPIFL LHHAFIDSIF ERWLRTHQPP RSIYPRTNAP 

       430        440        450        460        470        480 
IGHNDGYYMV PFLPLYRNGD YLLSNKALGY EYAYLLDPGQ RFVQEFLTPY LQQAQQIWQW 

       490        500        510        520        530        540 
LLGAGILGAL IATIVAAVIV FARRKRRRNQ KRKRAPSFGE RQPLLQSSSE EGSSSYQTTL

P55025 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools